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AB225295

Alexa Fluor® 488 Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

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(1 Publication)

Rabbit Recombinant Monoclonal beta 2 Adrenergic Receptor antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ADRB2R, B2AR, ADRB2, Beta-2 adrenergic receptor, Beta-2 adrenoreceptor, Beta-2 adrenoceptor

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (AB225295)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] (AB225295)

IHC image of beta 2 Adrenergic Receptor staining in a section of formalin-fixed paraffin-embedded normal human stomach*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Biocare Medical NxGen pressure cooker using retrieval settings of 110oC for 20 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab225295 at 1/100 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

  • Unconjugated

    Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)]

  • Carrier free

    Anti-beta 2 Adrenergic Receptor antibody [EPR707(N)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR707(N)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The beta 2 adrenergic receptor (also known as beta-2 adrenergic receptor B2 adrenergic receptor beta-2 receptor) is a transmembrane protein that acts as a receptor for catecholamines like adrenaline and noradrenaline. It belongs to the G protein-coupled receptors (GPCRs) superfamily and is involved in signal transduction by activating adenylate cyclase through the Gs protein. The mass of the beta 2 adrenergic receptor is approximately 46 kDa. This receptor is expressed in various tissues including the lungs skeletal muscle liver and smooth muscle.
Biological function summary

The beta 2 adrenergic receptor is important for mediating the physiological responses to adrenaline and noradrenaline. It plays a significant role in bronchodilation and vasodilation by relaxing airway and vascular smooth muscles. While it does not form a part of any complex its signaling initiates a cascade of intracellular events leading to changes in cellular function. This receptor ensures tissue responsiveness to catecholamines influencing cardiac output metabolic rate and smooth muscle relaxation.

Pathways

This receptor is an important component of the adrenergic signaling pathway and is closely associated with the cAMP-dependent signaling pathway. The activation of the beta 2 adrenergic receptor increases intracellular cAMP levels resulting in the activation of protein kinase A (PKA) and subsequent phosphorylation of various target proteins. The receptor interacts with other proteins within these pathways such as adenylate cyclase and PKA ensuring the propagation of downstream signaling that modulates cellular responses.

The beta 2 adrenergic receptor has a notable connection to asthma and chronic obstructive pulmonary disease (COPD). Its role in bronchodilation makes it a therapeutic target for managing these respiratory conditions. Alterations in the function or expression of the beta 2 adrenergic receptor can exacerbate these diseases. Additionally these disorders demonstrate a link with the muscarinic acetylcholine receptors which also affect airway tone regulation. Understanding the interactions between these receptors is essential for developing therapeutic strategies for respiratory diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.
See full target information ADRB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 13: PubMed33809455

2021

NFκB-Activated COX2/PGE/EP4 Axis Controls the Magnitude and Selectivity of BCG-Induced Inflammation in Human Bladder Cancer Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Omar M Ibrahim,Per H Basse,Weijian Jiang,Khurshid Guru,Gurkamal Chatta,Pawel Kalinski
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com