AB201831

Alexa Fluor® 488 Anti-beta I Tubulin antibody [EPR16778]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal TBB1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Tubulin beta-1 chain, TUBB1

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta I Tubulin antibody [EPR16778] (AB201831)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta I Tubulin antibody [EPR16778] (AB201831)

ab201831 staining beta I Tubulin in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201831 at a 1/50 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

Unconjugated

Anti-beta I Tubulin antibody [EPR16778]
Carrier free

Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16778

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Hamster": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Monkey": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta I Tubulin also known as TUBB1 is an essential component of the microtubule cytoskeleton specifically expressed in hematopoietic cells such as platelets. It is a protein with a mass of approximately 50 kDa. This protein shares structural similarities with other beta tubulins forming heterodimers with alpha-tubulin to construct the microtubules necessary for cell shape and movement. Beta I Tubulin primarily localizes to regions where it aids in the dynamics of microtubule assembly and stabilization.

Biological function summary

Beta tubulins are important for proper cell division signal transduction and organelle positioning. Beta I Tubulin integrates into the microtubule complex playing an important role in platelet formation and function. The dynamic behavior of microtubules facilitated by beta I Tubulin and its interactions influences cytoskeletal organization enabling cells to maintain their architecture and transport intracellular components effectively.

Pathways

Beta I Tubulin becomes critical in signaling pathways that involve cytoskeleton dynamics such as the Rho GTPase signaling pathway which is essential for actin and microtubule dynamics. It works together with proteins like alpha-tubulin and actin to ensure the proper development of megakaryocytes precursors to platelets. The function of these pathways determines efficient platelet production and stabilization closely linked to overall cellular homeostasis and response to external stimuli.

Beta I Tubulin has connections to conditions such as congenital macrothrombocytopenia and other platelet disorders. Mutations or dysregulations of beta I Tubulin can lead to abnormal platelet morphology and function impacting hemostasis. This protein also shows connections to alpha-tubulin in these disorders indicating the essential partnership between tubulin subunits in the formation of functional microtubules and emphasizing their significance in maintaining normal physiological states.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBB1

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:3504279 PubMed36062186

2022

Astrocytic IGF-1 and IGF-1R Orchestrate Mitophagy in Traumatic Brain Injury via Exosomal miR-let-7e.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Dabin,Chen Wei,Shu Liang,Cao Ke,Wang Zhihan,Zheng Ping

Aging 14:7348-7363 PubMed35852867

2022

RP11-552D4.1: a novel m6a-related LncRNA associated with immune status in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zheng,Xiaoxue Zhang,Dabin Ren,Yisong Zhang

Oxidative medicine and cellular longevity 2022:3522492 PubMed35571247

2022

circHtra1/miR-3960/GRB10 Axis Promotes Neuronal Loss and Immune Deficiency in Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zheng,Liang Shu,Dabin Ren,Zhucai Kuang,Yisong Zhang,Jian Wan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-beta I Tubulin antibody [EPR16778]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta I Tubulin antibody [EPR16778] (AB201831)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise