Rabbit Recombinant Monoclonal Beta-3-tubulin antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [EPR19591] (AB237350), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-beta-iii-tubulin-antibody-epr19591-ab237350--immunocytochemistry-immunofluorescence-img79279.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [EPR19591] (AB237350)")
Publications
- Nucleic acids research 50:e1192022A toolkit for the identification of NEAT1_2/paraspeckle modulators.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaiyan An,Karen T Elvers,Jason A Gillespie,Kimberley Jones,John R Atack,Olivera Grubisha,Tatyana A ShelkovnikovaPubMed 36099417
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes This product gave a positive signal in SHSY5Y fixed with 100% methanol (5 min) and 4% formaldehyde (10 min). |
Associated Products
Select an associated product type
8 products for Alternative Version
6 products for Alternative Product
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
Alternative names
Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3
Recommended products
Rabbit Recombinant Monoclonal Beta-3-tubulin antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.
Alternative names
Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR19591
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [EPR19591] (ab237350), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-beta-iii-tubulin-antibody-epr19591-ab237350--immunocytochemistry-immunofluorescence-img79279.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [EPR19591] (ab237350)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-beta III Tubulin antibody [EPR19591] (ab237350)
ab237350 staining beta III Tubulin in SH-SY5Y cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237350 at 1/100 dilution (shown in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in SH-SY5Y cells fixed with 4% formaldehyde (10 min).
Downloads
Product protocols
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