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AB220074

Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)]

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(6 Publications)

Anti-BrdU antibody [BU1/75 (ICR1)] - Alexa Fluor® 488 conjugated (ab220074) is a rat monoclonal antibody detecting BrdU in ICC/IF.

View Alternative Names

BUdr, Bromodeoxyuridine

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] (AB220074)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] (AB220074)

ab220074 staining BrdU in HeLa cells. Untreated and BrdU treated (10uM for 24 hours) cells were fixed with 100% methanol (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab220074 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

  • Carrier free

    Anti-BrdU antibody [BU1/75 (ICR1)] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-BrdU antibody [BU1/75 (ICR1)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-BrdU antibody [BU1/75 (ICR1)]

  • HRP

    HRP Anti-BrdU antibody [BU1/75 (ICR1)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-BrdU antibody [BU1/75 (ICR1)]

  • Unconjugated

    Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

BU1/75 (ICR1)

Isotype

IgG2a

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

ICC/IF

applications

Specificity

This antibody reacts with BrdU in single stranded DNA, BrdU attached to a protein carrier or free BrdU. It detects nucleated cells in S-Phase which have had BrdU incorporated into their DNA. Also reacts with chlorodeoxyuridine but with reduced staining. The antibody does not react with thymidine. It has been reported in the literature that this antibody clone cross-reacts with Edu (PMID: 23272138) and some customers reported that it cross reacts with IdU.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in HeLa cells treated with BrdU (10uM for 24 hours) fixed with 4% formaldehyde (10 min) and 100% methanol (5 min)</p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220074) is a rat monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF). Related products
Antibody clone BU1/75 (ICR1) is also available pre-conjugated to a variety of labels for your convenience – Anti-BrdU Alexa Fluor® 488 [BU1/75 (ICR1)] (ab220074).

Other related products
We have a range of other formats of antibody clone [BU1/75 (ICR1)] also available for your convenience: ab6326, Alexa Fluor® 488 - ab220074, Alexa Fluor® 647 - ab220075, Alexa Fluor® 594 - ab220076, HRP - ab220507, Alexa Fluor® 555 - ab221240, Carrier free - ab264079

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BrdU short for Bromodeoxyuridine is a synthetic analog of the nucleoside thymidine. It substitutes thymidine in newly synthesized DNA allowing for the tracking of cellular proliferation. Bromodeoxyuridine integrates into the DNA of proliferating cells which can then be detected using specific anti-BrdU antibodies. These antibodies are valuable tools in various assays such as BrdU staining and BrdU proliferation assay. BrdU has a molecular weight of approximately 315.13 g/mol. It is typically utilized in tissues or cell lines where active DNA synthesis and cellular proliferation are occurring.
Biological function summary

Bromodeoxyuridine acts as a marker for identifying cells in the S-phase of the cell cycle. During this phase DNA synthesis occurs and BrdU incorporates into the replicating DNA strand. When used in combination with anti-BrdU antibodies it enables researchers to identify and quantify dividing cells. This capability makes BrdU incorporation a widely accepted method to study cell proliferation in various biological samples. It functions independently without forming larger complexes.

Pathways

Bromodeoxyuridine integration highlights its role in DNA replication and repair pathways. It helps in the study of cell growth regulation pathways where proteins like PCNA (Proliferating Cell Nuclear Antigen) are involved. BrdU pairing with anti-BrdU antibodies allows assessment of DNA synthesis therefore aiding in the investigation of critical pathways like the cell cycle regulation pathway. In this context BrdU kits provide a consistent method for assessing dynamic changes in proliferative states.

Bromodeoxyuridine is useful in cancer research and neurological disorders like Alzheimer's disease. Through its ability to track cell proliferation researchers can explore cancerous tissue's growth patterns elucidating pathways and factors driving tumor expansion. BrdU assays contribute to understanding the aberrant cellular proliferation seen in tumors. For Alzheimer's disease BrdU aid in investigating neurogenesis rates particularly in regions affected by the disorder. Researchers often connect BrdU results with proteins like tau and amyloid-beta which play roles in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information BrdU
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 29:202-215 PubMed36401601

2022

Intermittent hypoxic conditioning restores neurological dysfunction of mice induced by long-term hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Gaifen Li,Yuying Guan,Yakun Gu,Mengyuan Guo,Wei Ma,Qianqian Shao,Jia Liu,Xunming Ji

Scientific reports 12:6169 PubMed35418189

2022

Aberrant R-loop-induced replication stress in MED12-mutant uterine fibroids.

Applications

Unspecified application

Species

Unspecified reactive species

Sribalasubashini Muralimanoharan,Ross Shamby,Nicholas Stansbury,Robert Schenken,Barbara de la Pena Avalos,Samin Javanmardi,Eloise Dray,Patrick Sung,Thomas G Boyer

International journal of molecular sciences 23: PubMed35163700

2022

The SDF1-CXCR4 Axis Is Involved in the Hyperbaric Oxygen Therapy-Mediated Neuronal Cells Migration in Transient Brain Ischemic Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ray-Yau Wang,Yea-Ru Yang,Heng-Chih Chang

iScience 25:103605 PubMed35005550

2022

Intracellular IL-32 regulates mitochondrial metabolism, proliferation, and differentiation of malignant plasma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kristin Roseth Aass,Robin Mjelle,Martin H Kastnes,Synne S Tryggestad,Luca M van den Brink,Ingrid Aass Roseth,Marita Westhrin,Muhammad Zahoor,Siv H Moen,Tonje M Vikene Nedal,Glenn Buene,Kristine Misund,Anne-Marit Sponaas,Qianli Ma,Anders Sundan,Richard Wj Groen,Tobias S Slørdahl,Anders Waage,Therese Standal

Aging 13:22528-22543 PubMed34555811

2021

Inhibition of FSTL3 abates the proliferation and metastasis of renal cell carcinoma via the GSK-3β/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fengfeng Sun,Peng Sun,Xiaofeng Yang,Liangliang Hu,Jianguo Gao,Tao Tian

Biomedicines 9: PubMed33801826

2021

Soluble JAM-C Ectodomain Serves as the Niche for Adipose-Derived Stromal/Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Morio Yamazaki,Kotaro Sugimoto,Yo Mabuchi,Rina Yamashita,Naoki Ichikawa-Tomikawa,Tetsuharu Kaneko,Chihiro Akazawa,Hiroshi Hasegawa,Tetsuya Imura,Hideki Chiba
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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