AB190026

Alexa Fluor® 488 Anti-c-Myc antibody [Y69]

RabMAb
Recombinant
KO Validated
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(7 Publications)

Rabbit Recombinant Monoclonal C-Myc/MYC antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (AB190026)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (AB190026)

ab190026 staining c-Myc in wild-type HEK293 cells (top panel) and MYC knockout HEK293 cells (ab256500) (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190026 at 1/5000 dilution and ab190573 (Rabbit monoclonal to alpha Tubulin - Alexa Fluor^® 647) at 1/250 dilution overnight at 4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (AB190026)

ab190026 staining c-myc in Panc-1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab190026 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

519 FITC

FITC Anti-c-Myc antibody [Y69]
HRP

HRP Anti-c-Myc antibody [Y69]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-c-Myc antibody [Y69]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-c-Myc antibody [Y69]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-c-Myc antibody [Y69]
Unconjugated

Anti-c-Myc antibody [Y69] - ChIP Grade
Carrier free

Anti-c-Myc antibody [Y69] - ChIP Grade - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

Y69

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in Panc1 cells fixed with 4% formaldehyde (10 min)</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

We also offer a PBS only version of this clone as product ab168727.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.

Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed : 24940000, PubMed : 25956029). Activates the transcription of growth-related genes (PubMed : 24940000, PubMed : 25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed : 24940000, PubMed : 25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed : 20010808).

See full target information MYC

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37762644

2023

Nuclear NPM-ALK Protects Myc from Proteasomal Degradation and Contributes to Its High Expression in Cancer Stem-Like Cells in ALK-Positive Anaplastic Large Cell Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chuquan Shang,Justine Lai,Moinul Haque,Will Chen,Peng Wang,Raymond Lai

American journal of cancer research 13:485-497 PubMed36895982

2023

POM121 promotes the proliferation and metastasis of gastric cancer via PI3K/AKT/MYC pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Changyuan Kang,Lizhou Jia,Lei Hao,Ning Zhang,Yang Liu,Lingli Zhang

BMC pulmonary medicine 22:443 PubMed36434577

2022

CircRNA has_circ_0017109 promotes lung tumor progression via activation of Wnt/β-catenin signaling due to modulating miR-671-5p/FZD4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yang,Bin Zhang,Qi Qi,Changli Wang

Annals of translational medicine 9:1722 PubMed35071416

2022

Aloe-emodin inhibits the proliferation, migration, and invasion of melanoma cells via inactivation of the Wnt/beta-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Maotao Du,Pan Shen,Ranjing Tan,Dengyan Wu,Shenghao Tu

Neuroscience research : PubMed33940081

2021

LncRNA MIR31HG is activated by STAT1 and facilitates glioblastoma cell growth via Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Runan Zhang,Dongmei Wu,Ying Wang,Liping Wu,Guowei Gao,Dayong Shan

OncoTargets and therapy 13:7137-7149 PubMed32801746

2020

LINC00501 Inhibits the Growth and Metastasis of Lung Cancer by Mediating miR-129-5p/HMGB1.

Applications

Unspecified application

Species

Unspecified reactive species

Ruo-Tian Wang,Yi Zhang,Shu-Yang Yao,Xiao-Gang Tan

Experimental and therapeutic medicine 18:3431-3438 PubMed31602218

2019

Wnt/β-catenin signaling pathway regulates asthma airway remodeling by influencing the expression of c-Myc and cyclin D1 via the p38 MAPK-dependent pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Xiao Jia,Ting-Ting Zhu,Yue Huang,Xin-Xin Zeng,Hong Zhang,Wei-Xi Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-c-Myc antibody [Y69]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (AB190026)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-c-Myc antibody [Y69] (AB190026)

Related conjugates and formulations (7)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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