JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB319184

Alexa Fluor® 488 Anti-CCR9 antibody [EPR26524-59]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal CCR9 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CDw199, GPR28, CCR9, C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, G-protein coupled receptor 28, GPR-9-6

1 Images
Flow Cytometry - Alexa Fluor® 488 Anti-CCR9 antibody [EPR26524-59] (AB319184)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-CCR9 antibody [EPR26524-59] (AB319184)

Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte from peripheral blood, Left) / MOLT-4 (human lymphoblastic leukemia T lymphoblast, Right) cells labelling CCR9 with ab319184 at 1/500 dilution (0.1ug) (Red) compared with a ab199091) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Negative control : Jurkat (PMID : 19525985).
Gated on viable cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26524-59

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR9 also known as CD199 is a chemokine receptor known for its role in guiding cell traffic. It is a G-protein-coupled receptor with a mass of approximately 43 kDa. CCR9 is mainly expressed on the surface of thymocytes T lymphocytes and some subsets of dendritic cells. Its presence is especially noted in the small intestine correlated with its role in the migration and function of immune cells in the gut. This receptor is important for the homing of these cells to the intestinal mucosa engaging specifically with its ligand CCL25.
Biological function summary

CCR9 plays a significant role in the immune system particularly in the gut. It is part of chemokine receptor-ligand complexes that are integral to leukocyte trafficking. By interacting with CCL25 CCR9 facilitates the directed movement of immune cells across intestinal tissue impacting the immune surveillance and response within this environment. The receptor's expression pattern also suggests involvement in thymocyte development supporting the immune system's ability to generate a diverse repertoire of T cells.

Pathways

CCR9 is an important component of the chemokine signaling pathway. It is intricately connected to G-protein signaling events triggering downstream effects that drive cell migration and adhesion. This pathway is important for the function and maintenance of mucosal immunity linking CCR9 with CCR7 and its role in guiding T cell migration to specific tissue sites. These pathways collectively support immune functionality and homeostasis underlining CCR9's significance in tissue-specific immunity.

CCR9 has associations with inflammatory disorders like Crohn's disease and ulcerative colitis. Inflammatory bowel disease (IBD) is marked by aberrant immune cell movement and function areas where CCR9 is particularly influential. The receptor's interaction with CCL25 is pivotal in these pathological conditions often being a therapeutic target to modulate immune cell trafficking in disease management. Additionally the altered expression of CCR9 may contribute to the progression of specific T-cell leukemias further emphasizing its clinical relevance.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.. (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.
See full target information CCR9
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB256268

Alexa Fluor® 488 Anti-CD1a antibody [NA1/34]

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD1a antibody [NA1/34] (AB256268)

  • 0
  • 0
Primary Antibodies

AB203829

Anti-ZEB1 antibody [EPR17375]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-ZEB1 antibody [EPR17375] (AB203829)

  • 3
  • 3
Primary Antibodies

AB134080

Anti-CD30 antibody [EPR4102]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [EPR4102] (AB134080)

  • 0
  • 0
Primary Antibodies

AB47003

Anti-Cardiac Troponin I antibody

BOND RX™ Validated

Western blot - Anti-Cardiac Troponin I antibody (AB47003)

  • 5
  • 17

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com