JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB187575

Alexa Fluor® 488 Anti-CD105 antibody [MEM226]

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Monoclonal CD105 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ENG.

View Alternative Names

CD105, END, ENG, Endoglin

1 Images
Flow Cytometry - Alexa Fluor® 488 Anti-CD105 antibody [MEM226] (AB187575)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 488 Anti-CD105 antibody [MEM226] (AB187575)

Surface staining of HUVEC (human umbilical vein endothelial cells) with ab187575 at 1/25 dilution

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM226

Isotype

IgG2a

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ENG.

P17813

style
left-column

Alternative Products

Primary Antibodies

AB11414

Anti-CD105 antibody [SN6]

Flow Cytometry - Anti-CD105 antibody [SN6] (AB11414)

  • 5
  • 6
Primary Antibodies

AB206419

Anti-CD105 antibody [EPR19911]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (AB206419)

  • 0
  • 0
Primary Antibodies

AB170943

Anti-CD105 antibody [EPR10145-10]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (AB170943)

  • 0
  • 0
Primary Antibodies

AB169545

Anti-CD105 antibody [EPR10145-12]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (AB169545)

  • 4
  • 3
Primary Antibodies

AB219362

Anti-CD105 antibody [EPR10145-12] - Low endotoxin, Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] - Low endotoxin, Azide free (AB219362)

  • 5
  • 1
Primary Antibodies

AB221675

Anti-CD105 antibody [EPR21846]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (AB221675)

  • 0
  • 0
Primary Antibodies

AB231832

Anti-CD105 antibody [EPR21846] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] - BSA and Azide free (AB231832)

  • 0
  • 0

Complementary Products

Assay Kits

AB217773

Human CD105 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD105 ELISA Kit (AB217773)

  • 0
  • 0
Primary Antibodies

AB202334

Alexa Fluor® 647 Anti-CD90 / Thy1 antibody [EPR3133]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-CD90 / Thy1 antibody [EPR3133] (AB202334)

  • 3
  • 1
Primary Antibodies

AB282789

PE Anti-CD73 antibody [EPR23048-139]

RabMAb|Recombinant

Flow Cytometry - PE Anti-CD73 antibody [EPR23048-139] (AB282789)

  • 0
  • 0
Primary Antibodies

AB223930

PE Anti-CD34 antibody [EP373Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-CD34 antibody [EP373Y] (AB223930)

  • 5
  • 1
Primary Antibodies

AB200317

Alexa Fluor® 647 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-CD45 antibody [EP322Y] (AB200317)

  • 0
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Use 4 μl for 100 μl of Human whole blood cells in suspension.</p><p><a href='/en-us/products/unavailable/mouse-igg2a-kappa-monoclonal-10d7a7b2-isotype-control-alexa-fluorreg-488-ab171464'>ab171464</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
ab187575 is conjugated with Alexa Fluor® 488 under optimum conditions and the conjugate purified by size-exclusion chromatography and adjusted for direct use.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.
Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis (PubMed : 21737454, PubMed : 23300529). Required for normal structure and integrity of adult vasculature (PubMed : 7894484). Regulates the migration of vascular endothelial cells (PubMed : 17540773). Required for normal extraembryonic angiogenesis and for embryonic heart development (By similarity). May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis (By similarity). May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors (PubMed : 1692830). Acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade that ultimately leads to the activation of SMAD transcription factors (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529, PubMed : 8370410). Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGFB1 signaling through SMAD3 (PubMed : 21737454, PubMed : 22347366, PubMed : 23300529).
See full target information ENG
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39519007

2024

Development and Characterization of a Human Mammary Epithelial Cell Culture Model for the Blood-Milk Barrier-A Contribution from the ConcePTION Project.

Applications

Unspecified application

Species

Unspecified reactive species

Debora La Mantia,Nina Nauwelaerts,Chiara Bernardini,Augusta Zannoni,Roberta Salaroli,Qi Lin,Isabelle Huys,Pieter Annaert,Monica Forni

NPJ Regenerative medicine 9:4 PubMed38242900

2024

hUC-MSCs-derived MFGE8 ameliorates locomotor dysfunction via inhibition of ITGB3/ NF-κB signaling in an NMO mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Huiming Xu,Wei Jiang,Xuejia Li,Jiaohua Jiang,Shabbir Khan Afridi,Longhui Deng,Rui Li,Ermei Luo,Zhaoqing Zhang,Yu-Wen Alvin Huang,Yaxiong Cui,Kwok-Fai So,Haijia Chen,Wei Qiu,Changyong Tang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com