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AB197701

Alexa Fluor® 488 Anti-CD11b antibody [M1/70]

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(6 Publications)

Rat Monoclonal CD11b antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse samples. Cited in 6 publications.

View Alternative Names

CD11b, Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Itgam

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (AB197701)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (AB197701)

ab197701 staining CD11b in RAW246.7 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197701 at 1µg/ml (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2 µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

  • Carrier free

    Anti-CD11b antibody [M1/70] - BSA and Azide free

  • Unconjugated

    Anti-CD11b antibody [M1/70]

  • 660 APC

    APC Anti-CD11b antibody [M1/70]

  • 660 APC

    APC Anti-CD11b antibody [M1/70]

  • 785 APC/Cy7®

    APC/Cy7® Anti-CD11b antibody [M1/70]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD11b antibody [M1/70]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD11b antibody [M1/70]

  • Biotin

    Biotin Anti-CD11b antibody [M1/70]

  • 519 FITC

    FITC Anti-CD11b antibody [M1/70]

  • 519 FITC

    FITC Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

  • 578 PE

    PE Anti-CD11b antibody [M1/70]

  • 667 PE/Cy5®

    PE/Cy5® Anti-CD11b antibody [M1/70]

  • 778 PE/Cy7®

    PE/Cy7® Anti-CD11b antibody [M1/70]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD11b antibody [M1/70]

  • 704 redFluor™ 710

    redFluor™ 710 Anti-CD11b antibody [M1/70]

  • 450 violetFluor™ 450

    violetFluor™ 450 Anti-CD11b antibody [M1/70]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

M1/70

Isotype

IgG2b

Light chain type

unknown

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Rabbit": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11b also known as Integrin alpha M is a protein with a molecular weight of approximately 170 kDa. This protein functions mechanically as an important adhesion molecule. It forms a complex with CD18 to become part of leukocyte-specific beta2 integrins specifically the Mac-1 (CR3) receptor. CD11b is largely expressed on the surface of various myeloid cells including monocytes macrophages granulocytes and a subset of natural killer cells. Researchers often utilize anti-CD11b antibodies and other markers such as APC or CD11b IHC to study its expression in different contexts.
Biological function summary

CD11b plays a critical role in various immune responses by mediating phagocytosis chemotaxis and cell migration. It is essential for proper leukocyte adherence to endothelial cells and for the engulfment of pathogens or apoptotic cells. CD11b as part of the Mac-1 complex contributes to the immune surveillance and inflammatory processes. This complex interacts with diverse ligands like ICAM-1 fibrinogen and complement component iC3b facilitating an immunological response.

Pathways

CD11b is instrumental within the integrin signaling pathway and the Leukocyte Transendothelial Migration pathway. Its interaction with ICAM-1 is central to the leukocyte adhesion cascade influencing the movement of immune cells across endothelial barriers. CD11b also associates with proteins such as Src family kinases and PI3K which activate downstream signaling responsible for the reorganization of the cytoskeleton and cell movement during immune responses.

CD11b is linked to inflammatory conditions like rheumatoid arthritis and systemic lupus erythematosus. Aberrant expression or function of CD11b can result in improper immune regulation contributing to the pathogenesis of these diseases. Its interaction with complement receptor iC3b plays a part in inflammation and tissue damage observed in these conditions. Consequently CD11b represents a potential target for therapeutic intervention in diseases where dysregulated immune activity is a feature.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity). It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (PubMed : 18685038).
See full target information Itgam
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:354 PubMed40316546

2025

KLF7-regulated ITGA2 as a therapeutic target for inhibiting oral cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Qi,Jiang Zhou,Pan Wang,Yunyan Li,Haoran Li,Yuwen Miao,XiaoQing Ma,Xiayan Luo,Zhiling Zhang,Yanling He,Wenyi Shen,Wenquan Zhao,Rutao Cui,Cang Li,Huiyong Zhu,Jiong Lyu

Frontiers in cell and developmental biology 13:1543710 PubMed40143972

2025

The effect of Polybrominated diphenyl ethers at the fetal blood-brain-barrier: evaluation using a microphysiological system.

Applications

Unspecified application

Species

Unspecified reactive species

Sourabh Sharma,Manuel Vidal,Souvik Paul,Arum Han,Ramkumar Menon,Lauren S Richardson

Science advances 10:eadn2806 PubMed39303037

2024

Defining metabolic flexibility in hair follicle stem cell induced squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Carlos Galvan,Aimee A Flores,Victoria Cerrilos,Itzetl Avila,Conor Murphy,Wilson Zheng,Heather R Christofk,William E Lowry

Cell proliferation 57:e13555 PubMed37748771

2023

Dissecting the immune discrepancies in mouse liver allograft tolerance and heart/kidney allograft rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Pan,Fang Ye,Hui Li,Chengxuan Yu,Jiajia Mao,Yanyu Xiao,Haide Chen,Junqing Wu,Jiaqi Li,Lijiang Fei,Yijun Wu,Xiaoming Meng,Guoji Guo,Yingying Wang

American journal of reproductive immunology (New York, N.Y. : 1989) 88:e13638 PubMed36308737

2022

Adapting an organ-on-chip device to study the effect of fetal sex and maternal race/ethnicity on preterm birth related intraamniotic inflammation leading to fetal neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren S Richardson,Nkechinyere Emezienna,Irina Burd,Brandie D Taylor,Morgan R Peltier,Arum Han,Ramkumar Menon

International journal of molecular sciences 23: PubMed35055018

2022

Ovarian Cancer Cells in Ascites Form Aggregates That Display a Hybrid Epithelial-Mesenchymal Phenotype and Allows Survival and Proliferation of Metastasizing Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sonia Capellero,Jessica Erriquez,Chiara Battistini,Roberta Porporato,Giulia Scotto,Fulvio Borella,Maria F Di Renzo,Giorgio Valabrega,Martina Olivero
View all publications
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Product promise

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