AB33503

Alexa Fluor® 488 Anti-CD11c antibody [N418]

Name: Anti-CD11c Alexa Fluor® 488 antibody [N418] (ab33503) IgG | Abcam
Brand: Abcam Limited
SKU: AB33503
Price: 225 USD
Availability: InStock

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(3 Publications)

Armenian hamster Monoclonal CD11c antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 3 publications.

View Alternative Names

CD11c, Integrin alpha-X, CD11 antigen-like family member C, Itgax

1 Images

Flow Cytometry - Alexa Fluor® 488 Anti-CD11c antibody [N418] (AB33503)

Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 488 Anti-CD11c antibody [N418] (AB33503)

C57BL/6 mouse splenocytes stained with ab33503 (right) or Armenian Hamster IgG Alexa Fluor^® 488 (monoclonal) (left). C57BL/6 Mouse splenocytes were incubated for 30 min on ice in 1x PBS containing 10 μg/ml anti CD16/32 antibody and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions followed by the antibody (ab33503) or Armenian Hamster IgG 488 Isotype (1x10⁶ in 100 μl at 1 : 500 (1 μg/ml)) for 30 min on ice. The cells were simultaneously stained with the following conjugated antibodies : MHC Class II PerCP, CD19 APC and CD3 PE.

Acquisition of >50,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Gating strategy : T and B cells were excluded from viable lymphocytes and expression of MHC Class II and CD11c was assessed.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD11c antibody [N418]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

N418

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

Clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD11c also known as ITGAX is a subunit of integrin proteins that plays a pivotal role in immune cell function. This protein has a molecular mass of about 150 kDa and is primarily expressed on the surface of dendritic cells monocytes and macrophages. It serves as a marker for these cells commonly utilized in applications like CD11c flow cytometry and CD11c IHC. Researchers often study this target using CD11c antibodies to explore immune system dynamics and cell surface characteristics.

Biological function summary

CD11c functions as an integrin alpha X subunit forming a heterodimer with the beta 2 subunit known as ITGB2. This complex is important for cell adhesion and migration processes. It mediates the binding of leukocytes to endothelial cells facilitating their migration from the bloodstream to sites of inflammation or infection. CD11c's presence on immune cells makes it essential for presenting antigens and initiating immune responses therefore having critical implications for immunity.

Pathways

CD11c plays a significant role in the immune system particularly in the leukocyte adhesion and migration pathways. It partners with proteins like ITGB2 to mediate these functions. The signaling pathways involving CD11c and related molecules regulate leukocyte trafficking and activation ensuring proper localization and function at sites of damage or infection. These pathways are essential for maintaining immune homeostasis and prompt responses to pathogens.

CD11c has significant connections to autoimmune diseases and chronic inflammatory conditions. Aberrant CD11c expression or activity can contribute to the pathogenesis of conditions like rheumatoid arthritis where leukocyte migration and persistent inflammation play a role. Studies also link CD11c with proteins involved in these disorders highlighting its importance in disease progression and potential as a therapeutic target. The understanding of CD11c's role in these conditions can guide the development of treatments aimed at modulating immune responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis (By similarity).

See full target information Itgax

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:6022-6043 PubMed40365283

2025

pH-responsive nano-vaccine combined with anti-PD-1 antibodies for enhanced immunotherapy of breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Wang,Hong Yu,Jianqiao Yin,Xiaopeng Yu

The Journal of biological chemistry 297:101232 PubMed34582891

2021

GPNMB plays a protective role against obesity-related metabolic disorders by reducing macrophage inflammatory capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Adam Prabata,Koji Ikeda,Elda Putri Rahardini,Ken-Ichi Hirata,Noriaki Emoto

Nature communications 7:10463 PubMed26781088

2016

TRAIP/RNF206 is required for recruitment of RAP80 to sites of DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Nam Soo Lee,Hee Jin Chung,Hyoung-June Kim,Seo Yun Lee,Jae-Hoon Ji,Yoojeong Seo,Seung Hun Han,Minji Choi,Miyong Yun,Seok-Geun Lee,Kyungjae Myung,Yonghwan Kim,Ho Chul Kang,Hongtae Kim

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-CD11c antibody [N418]

Flow Cytometry - Alexa Fluor® 488 Anti-CD11c antibody [N418] (AB33503)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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Secondary Antibodies

Primary Antibodies

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Product promise