Alexa Fluor® 488 Anti-CD146 antibody [EPR3208]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD146 antibody [EPR3208] (AB196448)

Immunohistochemical analysis of formalin fixed paraffin embedded human skeletal muscle labelling CD146 with ab196448 at a concentration of 1/50 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. The primary antibody Alexa Fluor® 488 Anti-CD146 antibody [EPR3208] was incubated for 60 mins at room temperature. Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Negative control : no staining on human skeletal muscle. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD146 antibody [EPR3208] (AB196448)

ab196448 staining CD146 in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196448 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD146 antibody [EPR3208] (AB196448)

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling CD146 with ab196448 at a concentration of 1/50 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. The primary antibody Alexa Fluor® 488 Anti-CD146 antibody [EPR3208] was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Positive staining on endothelium of human tonsil. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC

Lab

Immunohistochemistry - Alexa Fluor® 488 Anti-CD146 antibody [EPR3208] (AB196448)

Immunohistochemical analysis of formalin fixed paraffin embedded human breast cancer labelling CD146 with ab196448 at a concentration of 1/50 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. The primary antibody Alexa Fluor® 488 Anti-CD146 antibody [EPR3208] was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Positive staining on human breast cancer. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).