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AB326536

Alexa Fluor® 488 Anti-CD146 antibody [P1H12]

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Mouse Monoclonal CD146 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD146, MUC18, MCAM, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma cell adhesion molecule, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18, S-endo 1 endothelial-associated antigen

1 Images
Flow Cytometry - Alexa Fluor® 488 Anti-CD146 antibody [P1H12] (AB326536)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-CD146 antibody [P1H12] (AB326536)

Separation of HUVEC cells stained using ab326536 (concentration in sample 5 μg/m, red-filled) from HUVEC cells stained using mouse IgG1 isotype control (MOPC-21) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml, same as CD146 Alexa Fluor® 488 concentration, black-dashed) in flow cytometry analysis (surface staining).

  • Unconjugated

    Anti-CD146 antibody [P1H12]

  • 660 APC

    APC Anti-CD146 antibody [P1H12]

  • 519 FITC

    FITC Anti-CD146 antibody [P1H12]

  • 578 PE

    PE Anti-CD146 antibody [P1H12]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD146 antibody [P1H12]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD146 antibody [P1H12]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

P1H12

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
See full target information MCAM
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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