AB218293

Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal

Name: Alexa Fluor® 488 conjugated Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293) | Abcam
Brand: Abcam Limited
SKU: AB218293
Price: 590 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(8 Publications)

Anti-CD163 antibody [EPR14643-36] - C-terminal - Alexa Fluor® 488 conjugated (ab218293) is a rabbit recombinant monoclonal antibody detecting CD163 in IHC-Fr. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD163, M130, Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor

1 Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (AB218293)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (AB218293)

IHC image of CD163 staining in a section of frozen normal human liver.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab218293 at 1/100 dilution (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

Biotin

Biotin Anti-CD163 antibody [EPR14643-36] - C-terminal
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD163 antibody [EPR14643-36] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14643-36

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>This product gave a positive signal in frozen human liver tissue fixed with 10% formaldehyde (10 min).</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-Fr) in Human samples.

Related products
Antibody clone EPR14643-36 is also available pre-conjugated to a variety of labels for your convenience – Anti-CD163 Alexa Fluor® 488 [EPR14643-36] - C-terminal (ab218293).

Other related products
We have a range of other formats of antibody clone [EPR14643-36] also available for your convenience: ab189915, Carrier free - ab215976, Alexa Fluor® 488 - ab218293, Alexa Fluor® 647 - ab218294, Biotin - ab218295

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.

Biological function summary

CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.

Pathways

CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.

CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.

See full target information CD163

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 21:2248-2259 PubMed39478175

2024

Quality control for single-cell analysis of high-plex tissue profiles using CyLinter.

Applications

Unspecified application

Species

Unspecified reactive species

Gregory J Baker,Edward Novikov,Ziyuan Zhao,Tuulia Vallius,Janae A Davis,Jia-Ren Lin,Jeremy L Muhlich,Elizabeth A Mittendorf,Sandro Santagata,Jennifer L Guerriero,Peter K Sorger

Cancer cell 41:871-886.e10 PubMed37059105

2023

Lymphocyte networks are dynamic cellular communities in the immunoregulatory landscape of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Giorgio Gaglia,Megan L Burger,Cecily C Ritch,Danae Rammos,Yang Dai,Grace E Crossland,Sara Z Tavana,Simon Warchol,Alex M Jaeger,Santiago Naranjo,Shannon Coy,Ajit J Nirmal,Robert Krueger,Jia-Ren Lin,Hanspeter Pfister,Peter K Sorger,Tyler Jacks,Sandro Santagata

Cell genomics 2: PubMed36419822

2022

Spatial intra-tumor heterogeneity is associated with survival of lung adenocarcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Hua-Jun Wu,Daniel Temko,Zoltan Maliga,Andre L Moreira,Emi Sei,Darlan Conterno Minussi,Jamie Dean,Charlotte Lee,Qiong Xu,Guillaume Hochart,Connor A Jacobson,Clarence Yapp,Denis Schapiro,Peter K Sorger,Erin H Seeley,Nicholas Navin,Robert J Downey,Franziska Michor

Cancer cell 39:1623-1642.e20 PubMed34739845

2021

Myeloid antigen-presenting cell niches sustain antitumor T cells and license PD-1 blockade via CD28 costimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Jaikumar Duraiswamy,Riccardo Turrini,Aspram Minasyan,David Barras,Isaac Crespo,Alizée J Grimm,Julia Casado,Raphael Genolet,Fabrizio Benedetti,Alexandre Wicky,Kalliopi Ioannidou,Wilson Castro,Christopher Neal,Amandine Moriot,Stéphanie Renaud-Tissot,Victor Anstett,Noémie Fahr,Janos L Tanyi,Monika A Eiva,Connor A Jacobson,Kathleen T Montone,Marie Christine Wulff Westergaard,Inge Marie Svane,Lana E Kandalaft,Mauro Delorenzi,Peter K Sorger,Anniina Färkkilä,Olivier Michielin,Vincent Zoete,Santiago J Carmona,Periklis G Foukas,Daniel J Powell,Sylvie Rusakiewicz,Marie-Agnès Doucey,Denarda Dangaj Laniti,George Coukos

Nature cancer 2:66-82 PubMed33738458

2021

Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anita K Mehta,Emily M Cheney,Christina A Hartl,Constantia Pantelidou,Madisson Oliwa,Jessica A Castrillon,Jia-Ren Lin,Katie E Hurst,Mateus de Oliveira Taveira,Nathan T Johnson,William M Oldham,Marian Kalocsay,Matthew J Berberich,Sarah A Boswell,Aditi Kothari,Shawn Johnson,Deborah A Dillon,Mikel Lipschitz,Scott Rodig,Sandro Santagata,Judy E Garber,Nadine Tung,José Yélamos,Jessica E Thaxton,Elizabeth A Mittendorf,Peter K Sorger,Geoffrey I Shapiro,Jennifer L Guerriero

Scientific data 6:323 PubMed31848351

2019

Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.

Applications

mIHC

Species

Human

Rumana Rashid,Giorgio Gaglia,Yu-An Chen,Jia-Ren Lin,Ziming Du,Zoltan Maliga,Denis Schapiro,Clarence Yapp,Jeremy Muhlich,Artem Sokolov,Peter Sorger,Sandro Santagata

Nature protocols 14:2900-2930 PubMed31534232

2019

Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Ziming Du,Jia-Ren Lin,Rumana Rashid,Zoltan Maliga,Shu Wang,Jon C Aster,Benjamin Izar,Peter K Sorger,Sandro Santagata

mBio 9: PubMed30459195

2018

Streptococcus agalactiae Induces Placental Macrophages To Release Extracellular Traps Loaded with Tissue Remodeling Enzymes via an Oxidative Burst-Dependent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan S Doster,Jessica A Sutton,Lisa M Rogers,David M Aronoff,Jennifer A Gaddy

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (AB218293)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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