Rabbit Recombinant Monoclonal CD163 antibody - conjugated to Alexa Fluor® 488. C-terminal. Suitable for IHC-Fr and reacts with Human samples. Cited in 6 publications.
Images
![Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (AB218293), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-cd163-antibody-epr14643-36-c-terminal-ab218293--immunohistochemistry-frozen-sections-img41087.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (AB218293)")
Publications
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- Cell genomics 2:2022Spatial intra-tumor heterogeneity is associated with survival of lung adenocarcinoma patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua-Jun Wu,Daniel Temko,Zoltan Maliga,Andre L Moreira,Emi Sei,Darlan Conterno Minussi,Jamie Dean,Charlotte Lee,Qiong Xu,Guillaume Hochart,Connor A Jacobson,Clarence Yapp,Denis Schapiro,Peter K Sorger,Erin H Seeley,Nicholas Navin,Robert J Downey,Franziska MichorPubMed 36419822
- Cancer cell 39:1623-1642.e202021Myeloid antigen-presenting cell niches sustain antitumor T cells and license PD-1 blockade via CD28 costimulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaikumar Duraiswamy,Riccardo Turrini,Aspram Minasyan,David Barras,Isaac Crespo,Alizée J Grimm,Julia Casado,Raphael Genolet,Fabrizio Benedetti,Alexandre Wicky,Kalliopi Ioannidou,Wilson Castro,Christopher Neal,Amandine Moriot,Stéphanie Renaud-Tissot,Victor Anstett,Noémie Fahr,Janos L Tanyi,Monika A Eiva,Connor A Jacobson,Kathleen T Montone,Marie Christine Wulff Westergaard,Inge Marie Svane,Lana E Kandalaft,Mauro Delorenzi,Peter K Sorger,Anniina Färkkilä,Olivier Michielin,Vincent Zoete,Santiago J Carmona,Periklis G Foukas,Daniel J Powell,Sylvie Rusakiewicz,Marie-Agnès Doucey,Denarda Dangaj Laniti,George CoukosPubMed 34739845
- Nature cancer 2:66-822021Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnita K Mehta,Emily M Cheney,Christina A Hartl,Constantia Pantelidou,Madisson Oliwa,Jessica A Castrillon,Jia-Ren Lin,Katie E Hurst,Mateus de Oliveira Taveira,Nathan T Johnson,William M Oldham,Marian Kalocsay,Matthew J Berberich,Sarah A Boswell,Aditi Kothari,Shawn Johnson,Deborah A Dillon,Mikel Lipschitz,Scott Rodig,Sandro Santagata,Judy E Garber,Nadine Tung,José Yélamos,Jessica E Thaxton,Elizabeth A Mittendorf,Peter K Sorger,Geoffrey I Shapiro,Jennifer L GuerrieroPubMed 33738458
- Scientific data 6:3232019Highly multiplexed immunofluorescence images and single-cell data of immune markers in tonsil and lung cancer.
- Nature protocols 14:2900-29302019Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiming Du et. al.PubMed 31534232
- mBio 9:2018Streptococcus agalactiae Induces Placental Macrophages To Release Extracellular Traps Loaded with Tissue Remodeling Enzymes via an Oxidative Burst-Dependent Mechanism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan S Doster et. al.PubMed 30459195
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | ICC/IF | |
|---|---|---|
| Human | Tested | Not recommended |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes This product gave a positive signal in frozen human liver tissue fixed with 10% formaldehyde (10 min). |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
14 products for Alternative Product
3 products for Alternative Version
Target data
Function
Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Alternative names
Scavenger receptor cysteine-rich type 1 protein M130, Hemoglobin scavenger receptor, M130, CD163
Recommended products
Rabbit Recombinant Monoclonal CD163 antibody - conjugated to Alexa Fluor® 488. C-terminal. Suitable for IHC-Fr and reacts with Human samples. Cited in 6 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Conjugation
Alexa Fluor® 488
- Excitation/Emission
Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR14643-36
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD163 known also as hemoglobin scavenger receptor is a membrane glycoprotein with a molecular weight of approximately 130 kDa. It is predominantly expressed by monocytes and macrophages which are types of immune cells. Located on the cell surface CD163 functions as a receptor that aids in the clearance of hemoglobin-haptoglobin complexes playing a critical role in maintaining iron homeostasis and protecting tissues from oxidative damage. CD163 is not limited to human cells only; researchers often study its function using animal models such as chimeric rodents and chimeric rats.
- Biological function summary
CD163 participates in anti-inflammatory processes and is linked to immune modulation. It plays a pivotal role in the resolution of inflammation and promotes the removal of damaged cells and tissues. Through its involvement in the endocytic pathway CD163 interacts with various ligands facilitating interactions with other proteins. In the context of research CD163 functionality can be analyzed using techniques like CD163 IHC CD163 ELISA and CD163 stain in both human and mouse models.
- Pathways
CD163 is integral to the inflammation and immune response pathways. It participates in the heme-oxygenase pathway which is essential in the breakdown of heme into biliverdin free iron and carbon monoxide. This pathway involvement is tightly linked with heme oxygenase-1 (HO-1) a cytoprotective enzyme that helps to mitigate inflammatory responses. The interaction with haptoglobin and related proteins provides further insight into its regulatory roles within the immune system.
- Associated diseases and disorders
CD163 is associated with conditions like atherosclerosis and acute inflammation disorders. Its elevated levels indicate macrophage activation observed in chronic inflammations such as cardiovascular diseases. In atherosclerosis CD163 expression correlates with the uptake of oxidized LDL connecting it to progression of the disease. Additionally soluble CD163 levels serve as a biomarker in conditions like chronic liver disease where it is linked to macrophage activation and fibrosis. Within these disorders proteins like LDL and inflammatory cytokines are closely associated with CD163 function and regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-cd163-antibody-epr14643-36-c-terminal-ab218293--immunohistochemistry-frozen-sections-img41087.jpg "Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)")
Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-CD163 antibody [EPR14643-36] - C-terminal (ab218293)
IHC image of CD163 staining in a section of frozen normal human liver.
The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab218293 at 1/100 dilution (shown in green) and counterstained using Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
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