AB197543

Alexa Fluor® 488 Anti-CD166 antibody [EPR2759(2)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal CD166 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF, Flow Cyt, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

CD166, MEMD, ALCAM, CD166 antigen, Activated leukocyte cell adhesion molecule

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD166 antibody [EPR2759(2)] (AB197543)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD166 antibody [EPR2759(2)] (AB197543)

ab197543 staining CD166 in SKNSH cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197543 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 2μg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

578 PE

PE Anti-CD166 antibody [EPR2759(2)]
HRP

HRP Anti-CD166 antibody [EPR2759(2)]
660 APC

APC Anti-CD166 antibody [EPR2759(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD166 antibody [EPR2759(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD166 antibody [EPR2759(2)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CD166 antibody [EPR2759(2)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD166 antibody [EPR2759(2)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD166 antibody [EPR2759(2)]
Unconjugated

Anti-CD166 antibody [EPR2759(2)]
Carrier free

Anti-CD166 antibody [EPR2759(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2759(2)

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD166 also known as ALCAM (activated leukocyte cell adhesion molecule) is a cell surface glycoprotein with a mass of approximately 105 kDa. This protein is widely expressed on various cell types including activated T-cells keratinocytes neurons and endothelial cells. In the immune system CD166 has roles in T-cell activation and migration. It contains five extracellular immunoglobulin-like domains which facilitate cell-cell interactions. Researchers often regard ALCAM-Z as an alternative form sharing structural elements with CD166 protein.

Biological function summary

CD166 mediates cell-cell adhesion and plays roles in neuronal development. It participates in the formation of synaptic junctions and affects neuronal differentiation. CD166 is also a component of cell signaling complexes influencing cell proliferation and migration. In addition it controls interactions between T-cells and dendritic cells impacting immune response. Its interactions with other adhesion molecules contribute to cellular communication and tissue integrity.

Pathways

CD166 functions within signaling pathways related to cell adhesion and migration. It is involved in the Wnt signaling pathway and its interactions regulate cell polarity and morphogenesis. CD166 interacts closely with proteins such as CD6 and Catenins impacting cellular signaling and the organization of the cytoskeleton. These pathways affect processes like embryonic development and immune cell function.

CD166 has implications in cancer and autoimmune diseases. Its dysregulation links to cancer metastasis where altered expression facilitates tumor invasion and spread. CD166 also relates to autoimmune diseases through its role in T-cell activation potentially contributing to conditions like multiple sclerosis. The interaction between CD166 and CD6 influences immune cell activation and may offer therapeutic targets for modulating immune responses in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts (PubMed : 15048703, PubMed : 15496415, PubMed : 16352806, PubMed : 23169771, PubMed : 24945728, PubMed : 7760007). Promotes T-cell activation and proliferation via its interactions with CD6 (PubMed : 15048703, PubMed : 16352806, PubMed : 24945728). Contributes to the formation and maturation of the immunological synapse via its interactions with CD6 (PubMed : 15294938, PubMed : 16352806). Mediates homotypic interactions with cells that express ALCAM (PubMed : 15496415, PubMed : 16352806). Acts as a ligand for the LILRB4 receptor, enhancing LILRB4-mediated inhibition of T cell proliferation (PubMed : 29263213). Required for normal hematopoietic stem cell engraftment in the bone marrow (PubMed : 24740813). Mediates attachment of dendritic cells onto endothelial cells via homotypic interaction (PubMed : 23169771). Inhibits endothelial cell migration and promotes endothelial tube formation via homotypic interactions (PubMed : 15496415, PubMed : 23169771). Required for normal organization of the lymph vessel network. Required for normal hematopoietic stem cell engraftment in the bone marrow. Plays a role in hematopoiesis; required for normal numbers of hematopoietic stem cells in bone marrow. Promotes in vitro osteoblast proliferation and differentiation (By similarity). Promotes neurite extension, axon growth and axon guidance; axons grow preferentially on surfaces that contain ALCAM. Mediates outgrowth and pathfinding for retinal ganglion cell axons (By similarity).. Isoform 3. Inhibits activities of membrane-bound isoforms by competing for the same interaction partners. Inhibits cell attachment via homotypic interactions. Promotes endothelial cell migration. Inhibits endothelial cell tube formation.

See full target information ALCAM

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature protocols 17:378-401 PubMed35022622

2022

IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues.

Applications

mIHC

Species

Human

Andrea J Radtke,Colin J Chu,Ziv Yaniv,Li Yao,James Marr,Rebecca T Beuschel,Hiroshi Ichise,Anita Gola,Juraj Kabat,Bradley Lowekamp,Emily Speranza,Joshua Croteau,Nishant Thakur,Danny Jonigk,Jeremy L Davis,Jonathan M Hernandez,Ronald N Germain

Proceedings of the National Academy of Sciences of the United States of America 117:33455-33465 PubMed33376221

2020

IBEX: A versatile multiplex optical imaging approach for deep phenotyping and spatial analysis of cells in complex tissues.

Applications

mIHC

Species

Human

Andrea J Radtke,Evelyn Kandov,Bradley Lowekamp,Emily Speranza,Colin J Chu,Anita Gola,Nishant Thakur,Rochelle Shih,Li Yao,Ziv Rafael Yaniv,Rebecca T Beuschel,Juraj Kabat,Joshua Croteau,Jeremy Davis,Jonathan M Hernandez,Ronald N Germain

Clinical and experimental immunology 198:1-10 PubMed31206174

2019

Decreased A20 expression on circulating CD56 NK cells contributes to a worse disease status in patients with ankylosing spondylitis.

Applications

Unspecified application

Species

Unspecified reactive species

M Yang,Y Zhou,L Liu,S Wang,J Jiang,Q Shang,H Yu,X Xiang,X Pang,T Li,P Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-CD166 antibody [EPR2759(2)]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD166 antibody [EPR2759(2)] (AB197543)

Related conjugates and formulations (10)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise