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Rat Monoclonal FCGR3 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt, ICC/IF and reacts with Mouse samples.

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Images

Flow Cytometry - Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (AB256417), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (AB256417), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Rat
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Mouse
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

This product gave a positive signal in Raw264.7 fixed with 100% methanol (5 min) and 4% formaldehyde (10 min).

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor which binds to IgG1, IgG2a and IgG2b (PubMed:17558411). Mediates neutrophil activation by IgG complexes redundantly with Fcgr4 (PubMed:18097064).

Additional Targets

Fcgr2

Alternative names

CD16, Low affinity immunoglobulin gamma Fc region receptor III, IgG Fc receptor III, Fc-gamma RIII, FcRIII, Fcgr3

Recommended products

Rat Monoclonal FCGR3 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt, ICC/IF and reacts with Mouse samples.

Key facts

Isotype
IgG2a
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
93
Purification technique
Affinity purification Protein G
Epitope
Reacts with a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors.
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark

Notes

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD16 and CD32 also referred to as FCγRIII and FCγRII are proteins expressed on the surface of various immune cells like natural killer cells macrophages and some subsets of T cells. CD16 has a mass ranging between 50-80 kDa while CD32 exhibits variability in size between 32-40 kDa due to different isoforms. These proteins play pivotal roles in immune response by binding to the Fc region of immunoglobulins influencing cell activation and signaling.

Biological function summary

CD16 and CD32 serve important roles in mediating immune cell interactions. They work as part of immune synapses facilitating antibody-dependent cellular cytotoxicity (ADCC) and phagocytosis processes. Due to their involvement in these complex activities they are included in immunological synapse formation and modulation extending their influence over overall immune regulation and response. These proteins are known to contribute to both innate and adaptive immune mechanisms as receptors for the Fc portion of immunoglobulin G.

Pathways

CD16 and CD32 interact within signaling pathways like the Fc gamma receptor-mediated phagocytosis pathway and the immune response-regulating signaling pathway. These receptors work in coordination with other immunoglobulin family members to initiate targeted cellular reactions necessary for immune challenge responses. CD32 often partners with CD19 and CD21 within pathways to modulate B cell receptor-mediated signaling.

Associated diseases and disorders

CD16 and CD32 have connections to autoimmune diseases and infectious diseases. Aberrant expression or dysfunction in these proteins can contribute to the development of conditions such as systemic lupus erythematosus where improper immune complex clearance occurs and chronic inflammation results. CD16's involvement with NK cells can also play a role in viral infection control highlighting their importance in pathogen defense mechanisms.

Product promise

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Terms & Conditions.

2 product images

  • Flow Cytometry - Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (ab256417), expandable thumbnail

    Flow Cytometry - Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (ab256417)

    Flow cytometry staining of C57 BL/6 mouse splenocytes stained with ab256417 (right) or Rat IgG2aκ Alexa Fluor® 488 isotype (left). Cells were incubated for 30 min on ice in 1x PBS containing 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256417) or Rat IgG2aκ Alexa Fluor® 488 isotype (1x 106 in 100 µl at 0.2 µg/ml (1/500)) for 30 min on ice. The cells were simultaneously stained with CD19.

    Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live single cells.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (ab256417), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD16+CD32 antibody [93] (ab256417)

    ab256417 staining CD16+CD32 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab256417 at 1/500 dilution (shown in green) and Alexa Fluor® 647 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in Raw264.7 cells fixed with 4% formaldehyde (10 min).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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