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AB319018

Alexa Fluor® 488 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87]

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Rabbit Recombinant Monoclonal C209B antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Mouse, Rat samples.

View Alternative Names

CD209, CD209 antigen-like protein B, DC-SIGN-related protein 1, OtB7, DC-SIGNR1, Cd209b

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (AB319018)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (AB319018)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling CD209B/DC-SIGNR1 with ab319018 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on mouse skeletal muscle.
The section was incubated with ab319018 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (AB319018)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (AB319018)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling CD209B/DC-SIGNR1 with ab319018 at 1/100 (5.0 ug/ml) dilution.

Negative control : no staining on rat skeletal muscle.
The section was incubated with ab319018 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (AB319018)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (AB319018)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CD209B/DC-SIGNR1 with ab319018 at 1/100 (5.0 ug/ml) dilution.

Positive staining on rat spleen.
The section was incubated with ab319018 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (AB319018)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87] (AB319018)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD209B/DC-SIGNR1 with ab319018 at 1/100 (5.0 ug/ml) dilution.

Positive staining on mouse spleen.
The section was incubated with ab319018 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD209B (DC-SIGNR1) antibody [EPR27427-87]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD209B/DC-SIGNR1 antibody [EPR27427-87]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27427-87

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD209B also known as DC-SIGNR1 is a C-type lectin receptor with a molecular mass of about 45 kDa. This receptor is similar in structure to CD209A another member of the same gene family. CD209B is mainly expressed on macrophages and dendritic cells which are critical components of the immune system. This receptor plays a role in the immune response by recognizing and binding to specific carbohydrate structures on the surface of various pathogens.
Biological function summary

CD209B functions as part of a broader set of protein interactions involved in pathogen recognition and clearance. It acts as a pattern recognition receptor and is involved in initiating immune responses. Although not part of a large complex CD209B shares functional similarities with other receptors like CD209A mediating defensive strategies against infections through binding to glycoprotein antigens on microbes.

Pathways

The receptor participates in the immune signaling networks that oversee pathogen recognition. It is vital within the innate immune pathway where it helps to stimulate adaptive immunity. CD209B engages with proteins such as Toll-like receptors which amplify immune responses against pathogens.

CD209B has potential implications in infectious diseases and autoimmune disorders. Its ability to bind diverse pathogens makes it an important player in infections like HIV where it helps the virus gain entry into host cells. Additionally its interaction with proteins like mannose receptor suggests a role in diseases characterized by chronic inflammation. Understanding these interactions assists in developing interventions to modulate immune response in such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable pathogen-recognition receptor. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. May recognize in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates.
See full target information Cd209b
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com