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AB253249

Alexa Fluor® 488 Anti-CD3 antibody [OKT3]

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(1 Publication)

Mouse Monoclonal CD3d antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images
Flow Cytometry - Alexa Fluor® 488 Anti-CD3 antibody [OKT3] (AB253249)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-CD3 antibody [OKT3] (AB253249)

Flow cytometric analysis (surface staining) of human peripheral blood leukocytes using ab253249.

  • Unconjugated

    Anti-CD3 antibody [OKT3]

  • Carrier free

    Anti-CD3 epsilon antibody [OKT3] - Low endotoxin, Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD3 antibody [OKT3]

  • redFluor™ 710

    redFluor™ 710 Anti-CD3 antibody [OKT3]

  • 660 APC

    APC Anti-CD3 epsilon antibody [OKT3]

  • Biotin

    Biotin Anti-CD3 antibody [OKT3]

  • 519 FITC

    FITC Anti-CD3 epsilon antibody [OKT3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OKT3

Isotype

IgG2a

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Epitope

extracellular

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "4 µL for 10^6 Cells", "FlowCyt-species-notes": "<p>Or 4 μL per 100 μL whole blood.</p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
The purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Storage buffer
pH: 7.4 Preservative: 0.0975% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36362214

2022

Characterization of BCMA Expression in Circulating Rare Single Cells of Patients with Plasma Cell Neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Libere J Ndacayisaba,Kate E Rappard,Stephanie N Shishido,Sonia M Setayesh,Guilin Tang,Pei Lin,Nicholas Matsumoto,Ching-Ju Hsu,Rafael Nevarez,Carmen Ruiz Velasco,Amin Naghdloo,Eric Yang,Kevin Kelly,James Hicks,Jeremy Mason,Robert Z Orlowski,Elisabet E Manasanch,Peter Kuhn
View all publications
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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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