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AB215911

Alexa Fluor® 488 Anti-CD31 antibody [JC/70A]

5

(1 Review)

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(13 Publications)

Anti-CD31 antibody [JC/70A] - Alexa Fluor® 488 conjugated (ab215911) is a mouse monoclonal antibody detecting CD31. Suitable for Human.

View Alternative Names

CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1

1 Images
Immunocytochemistry - Alexa Fluor® 488 Anti-CD31 antibody [JC/70A] (AB215911)
  • ICC

Lab

Immunocytochemistry - Alexa Fluor® 488 Anti-CD31 antibody [JC/70A] (AB215911)

ab215911 staining CD31 in HUVEC (Human umbilical vein endothelial cell line) cells. The cells were fixed with 100% methanol (5 minutes), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated overnight at +4°C with ab215911 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in HUVEC cells fixed with 4% formaldehyde (10 minutes).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

JC/70A

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100", "ICC-species-notes": "<p>This product gave a positive signal in HUVEC cells fixed with 4% formaldehyde (10 minutes) and 100% methanol (5 minutes).</p><p>Use 0.3M glycine to quench autofluorescence caused by aldehydes.</p><p>Positive Control: HUVEC cells</p>" }, "Cynomolgus monkey": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-CD31 antibody [JC/70A] (ab215911) is a mouse monoclonal antibody Related products
Antibody clone JC/70A is also available pre-conjugated to a variety of labels for your convenience – Anti-CD31 Alexa Fluor® 488 [JC/70A] (ab215911).

Other related products
We have a range of other formats of antibody clone [JC/70A] also available for your convenience: ab9498, PE - ab215660, Alexa Fluor® 488 - ab215911, Alexa Fluor® 647 - ab215912, Carrier free - ab264090

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed : 17580308, PubMed : 19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed : 19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed : 27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed : 17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed : 12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed : 12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed : 18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed : 18672896). Induces susceptibility to atherosclerosis (By similarity).. Isoform Delta15. Does not protect against apoptosis.
See full target information PECAM1
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Alzheimer's & dementia : the journal of the Alzheimer's Association 20:5590-5606 PubMed38958537

2024

Neurovascular coupling, functional connectivity, and cerebrovascular endothelial extracellular vesicles as biomarkers of mild cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Cameron D Owens,Camila Bonin Pinto,Peter Mukli,Rafal Gulej,Faddi Saleh Velez,Sam Detwiler,Lauren Olay,Jordan R Hoffmeister,Zsofia Szarvas,Mihaly Muranyi,Anna Peterfi,Ana Clara da C Pinaffi-Langley,Cheryl Adams,Jason Sharps,Zalan Kaposzta,Calin I Prodan,Angelia C Kirkpatrick,Stefano Tarantini,Anna Csiszar,Zoltan Ungvari,Ann L Olson,Guangpu Li,Priya Balasubramanian,Veronica Galvan,Andrew Bauer,Zachary A Smith,Tarun W Dasari,Shawn Whitehead,Manoj R Medapti,Fanny M Elahi,Aikaterini Thanou,Andriy Yabluchanskiy

Advanced functional materials 33: PubMed38558868

2024

Injectable Shear-Thinning Hydrogels with Sclerosing and Matrix Metalloproteinase Modulatory Properties for the Treatment of Vascular Malformations.

Applications

Unspecified application

Species

Unspecified reactive species

Fatemeh Zehtabi,Ankit Gangrade,Kaylee Tseng,Reihaneh Haghniaz,Reza Abasgholizadeh,Hossein Montazerian,Danial Khorsandi,Jamal Bahari,Amir Ahari,Neda Mohaghegh,Negar Hosseinzadeh Kouchehbaghi,Kalpana Mandal,Marvin Mecwan,Ahmad Rashad,Natan Roberto de Barros,Youngjoo Byun,Menekse Ermis,Han-Jun Kim,Ali Khademhosseini

Cancers 15: PubMed37835577

2023

Influence of Macrophages on Vascular Invasion of Inflammatory Breast Cancer Emboli Measured Using an In Vitro Microfluidic Multi-Cellular Platform.

Applications

Unspecified application

Species

Unspecified reactive species

Manasa Gadde,Melika Mehrabi-Dehdezi,Bisrat G Debeb,Wendy A Woodward,Marissa Nichole Rylander

Biosensors 13: PubMed37622905

2023

Rapid Quantification of Microvessels of Three-Dimensional Blood-Brain Barrier Model Using Optical Coherence Tomography and Deep Learning Algorithm.

Applications

Unspecified application

Species

Unspecified reactive species

Huiting Zhang,Dong-Hee Kang,Marie Piantino,Daisuke Tominaga,Takashi Fujimura,Noriyuki Nakatani,J Nicholas Taylor,Tomomi Furihata,Michiya Matsusaki,Satoshi Fujita

Nature methods 19:1449-1460 PubMed36280722

2022

Geometric engineering of organoid culture for enhanced organogenesis in a dish.

Applications

Unspecified application

Species

Unspecified reactive species

Sunghee Estelle Park,Shawn Kang,Jungwook Paek,Andrei Georgescu,Jeehan Chang,Alex Yoon Yi,Benjamin J Wilkins,Tatiana A Karakasheva,Kathryn E Hamilton,Dan Dongeun Huh

STAR protocols 3:101303 PubMed35496807

2022

Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking.

Applications

Unspecified application

Species

Unspecified reactive species

Gihyun Lee,Soo Jee Kim,Je-Kyun Park

Micromachines 12: PubMed34945377

2021

Modular 3D In Vitro Artery-Mimicking Multichannel System for Recapitulating Vascular Stenosis and Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Minkyung Cho,Je-Kyun Park

Antioxidants (Basel, Switzerland) 10: PubMed34679682

2021

Patient Endothelial Colony-Forming Cells to Model Coronary Artery Disease Susceptibility and Unravel the Role of Dysregulated Mitochondrial Redox Signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Marie Besnier,Meghan Finemore,Christine Yu,Katharine A Kott,Stephen T Vernon,Nicole A Seebacher,Elijah Genetzakis,Anamarija Furman,Owen Tang,Ryan L Davis,Thomas Hansen,Peter J Psaltis,Kristen J Bubb,Steven G Wise,Stuart M Grieve,Belinda A Di Bartolo,Gemma A Figtree

Frontiers in cell and developmental biology 9:733015 PubMed34621749

2021

Direct Evidence of Endothelial Dysfunction and Glycocalyx Loss in Dermal Biopsies of Patients With Chronic Kidney Disease and Their Association With Markers of Volume Overload.

Applications

Unspecified application

Species

Unspecified reactive species

Josephine Koch,Ryanne S Hijmans,Manuela Ossa Builes,Wendy A Dam,Robert A Pol,Stephan J L Bakker,Hendri H Pas,Casper F M Franssen,Jacob van den Born

Biotechnology and bioengineering 117:3572-3590 PubMed32648934

2020

In vitro vascularized tumor platform for modeling tumor-vasculature interactions of inflammatory breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Manasa Gadde,Caleb Phillips,Neda Ghousifam,Anna G Sorace,Enoch Wong,Savitri Krishnamurthy,Anum Syed,Omar Rahal,Thomas E Yankeelov,Wendy A Woodward,Marissa N Rylander
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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