Anti-CD31 [JC/70A] Alexa Fluor® 488 conjugated antibody (ab215911) mouse monoclonal antibody that is used to detect CD31. Suitable for Human samples.
Images
![Immunocytochemistry - Alexa Fluor® 488 Anti-CD31 antibody [JC/70A] (AB215911), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-cd31-antibody-jc-70a-ab215911--immunocytochemistry-img67802.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Alexa Fluor® 488 Anti-CD31 antibody [JC/70A] (AB215911)")
Publications
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- Cancers 15:2023Influence of Macrophages on Vascular Invasion of Inflammatory Breast Cancer Emboli Measured Using an In Vitro Microfluidic Multi-Cellular Platform.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManasa Gadde,Melika Mehrabi-Dehdezi,Bisrat G Debeb,Wendy A Woodward,Marissa Nichole RylanderPubMed 37835577
- Biosensors 13:2023Rapid Quantification of Microvessels of Three-Dimensional Blood-Brain Barrier Model Using Optical Coherence Tomography and Deep Learning Algorithm.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiting Zhang,Dong-Hee Kang,Marie Piantino,Daisuke Tominaga,Takashi Fujimura,Noriyuki Nakatani,J Nicholas Taylor,Tomomi Furihata,Michiya Matsusaki,Satoshi FujitaPubMed 37622905
- STAR protocols 3:1013032022Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGihyun Lee,Soo Jee Kim,Je-Kyun ParkPubMed 35496807
- Antioxidants (Basel, Switzerland) 10:2021Patient Endothelial Colony-Forming Cells to Model Coronary Artery Disease Susceptibility and Unravel the Role of Dysregulated Mitochondrial Redox Signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie Besnier,Meghan Finemore,Christine Yu,Katharine A Kott,Stephen T Vernon,Nicole A Seebacher,Elijah Genetzakis,Anamarija Furman,Owen Tang,Ryan L Davis,Thomas Hansen,Peter J Psaltis,Kristen J Bubb,Steven G Wise,Stuart M Grieve,Belinda A Di Bartolo,Gemma A FigtreePubMed 34679682
- Frontiers in cell and developmental biology 9:7330152021Direct Evidence of Endothelial Dysfunction and Glycocalyx Loss in Dermal Biopsies of Patients With Chronic Kidney Disease and Their Association With Markers of Volume Overload.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJosephine Koch,Ryanne S Hijmans,Manuela Ossa Builes,Wendy A Dam,Robert A Pol,Stephan J L Bakker,Hendri H Pas,Casper F M Franssen,Jacob van den BornPubMed 34621749
- Biotechnology and bioengineering 117:3572-35902020In vitro vascularized tumor platform for modeling tumor-vasculature interactions of inflammatory breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManasa Gadde,Caleb Phillips,Neda Ghousifam,Anna G Sorace,Enoch Wong,Savitri Krishnamurthy,Anum Syed,Omar Rahal,Thomas E Yankeelov,Wendy A Woodward,Marissa N RylanderPubMed 32648934
- Lab on a chip 20:2911-29262020HepaChip-MP - a twenty-four chamber microplate for a continuously perfused liver coculture model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarius Busche,Olena Tomilova,Julia Schütte,Simon Werner,Meike Beer,Nicola Groll,Britta Hagmeyer,Michael Pawlak,Peter D Jones,Christian Schmees,Holger Becker,Juliane Schnabel,Karsten Gall,Roland Hemmler,Madlen Matz-Soja,Georg Damm,Simon Beuck,Tobias Klaassen,Jana Moer,Anett Ullrich,Dieter Runge,Katja Schenke-Layland,Rolf Gebhardt,Martin StelzlePubMed 32662810
- Aging 12:12002-120182020Plasma endothelial cells-derived extracellular vesicles promote wound healing in diabetes through YAP and the PI3K/Akt/mTOR pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeng Wei et. al.PubMed 32570219
- Scientific reports 9:89202019Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiuseppe Pettinato et. al.PubMed 31222080
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC | |
|---|---|
| Human | Tested |
| Cynomolgus monkey | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes This product gave a positive signal in HUVEC cells fixed with 4% formaldehyde (10 minutes) and 100% methanol (5 minutes). Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
8 products for Alternative Product
Target data
Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:17580308, PubMed:19342684). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity). Isoform Delta15. Does not protect against apoptosis.
Alternative names
CD31, Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, PECAM1
Recommended products
Anti-CD31 [JC/70A] Alexa Fluor® 488 conjugated antibody (ab215911) mouse monoclonal antibody that is used to detect CD31. Suitable for Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- JC/70A
- Purification technique
- Affinity purification Immunogen
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Alexa Fluor® 488 Anti-CD31 antibody [JC/70A] (ab215911) is a mouse monoclonal antibody
Recommended positive controls
ICC: HUVEC cells
Related products
Antibody clone JC/70A is also available pre-conjugated to a variety of labels for your convenience – Anti-CD31 Alexa Fluor® 488 [JC/70A] (ab215911).
Other related products
We have a range of other formats of antibody clone [JC/70A] also available for your convenience:
Anti-CD31 antibody [JC/70A] ab9498, PE - PE Anti-CD31 antibody [JC/70A] ab215660, Alexa Fluor® 488 - ab215911, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-CD31 antibody [JC/70A] ab215912, Carrier free - Anti-CD31 antibody [JC/70A] - BSA and Azide free ab264090
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry - Alexa Fluor® 488 Anti-CD31 antibody [JC/70A] (ab215911), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-cd31-antibody-jc-70a-ab215911--immunocytochemistry-img67802.jpg "Immunocytochemistry - Alexa Fluor® 488 Anti-CD31 antibody [JC/70A] (ab215911)")
Immunocytochemistry - Alexa Fluor® 488 Anti-CD31 antibody [JC/70A] (ab215911)
ab215911 staining CD31 in HUVEC (Human umbilical vein endothelial cell line) cells. The cells were fixed with 100% methanol (5 minutes), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated overnight at +4°C with ab215911 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HUVEC cells fixed with 4% formaldehyde (10 minutes).
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