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AB196372

Alexa Fluor® 488 Anti-CD4 antibody [EPR6855]

4

(2 Reviews)

|

(8 Publications)

Anti-CD4 antibody [EPR6855] - Alexa Fluor® 488 conjugated (ab196372) is a rabbit recombinant monoclonal antibody detecting CD4 in IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (AB196372)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (AB196372)

Immunofluorescence staining of CD4 in a section of formalin-fixed paraffin-embedded human normal tonsil*.

The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9.0) using retrieval settings of 110°C for 40 minutes. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab196372 at 1/100 dilution (shown in green) and counterstained using ab17147 Ms anti-CD8 at 1/100 dilution. The section was then incubated with ab150118 ((Goat Anti-Mouse IgG H&L (Alexa Fluor® 555) preabsorbed) 1/1000, shown in red) for 1 hour at room temperature. DAPI was used to stain the cell nuclei (blue). The secondary-only control insert image is taken from an identical assay without primary antibody. The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6855

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-CD4 antibody [EPR6855] (ab196372) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Related products
Antibody clone EPR6855 is also available pre-conjugated to a variety of labels for your convenience – Anti-CD4 Alexa Fluor® 488 [EPR6855] (ab196372).

Other related products
We have a range of other formats of antibody clone [EPR6855] also available for your convenience: ab133616, Carrier free - ab181724, HRP - ab195842, Alexa Fluor® 647 - ab196147, Alexa Fluor® 488 - ab196372, Alexa Fluor® 594 - ab277931, Alexa Fluor® 555 - ab280849, ab284581, Carrier free - ab307492, ab317787, Carrier free - ab317797

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule : peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1) (PubMed : 12089508, PubMed : 16331979, PubMed : 2214026, PubMed : 9641677). Down-regulated by HIV-1 Vpu (PubMed : 17346169). Acts as a receptor for Human Herpes virus 7/HHV-7 (PubMed : 7909607).
See full target information CD4
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

World journal of surgical oncology 23:131 PubMed40205382

2025

Correlation analysis of tertiary lymphoid structure parameters with the prognosis of patients with locally advanced rectal cancer after neoadjuvant chemotherapy: a retrospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjian Jiang,Chuang Zhang,Yifei Hou,Bin Zhao,Binbin Cui

Biochemistry and biophysics reports 38:101716 PubMed38737726

2024

Function and expression of N-acetyltransferases 1 and 2 are altered in lymphocytes in type 2 diabetes and obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Víctor Alejandro Paz-Rodríguez,Diana Judith Herrera-Vargas,Eneida Turiján-Espinoza,Miguel Ernesto Martínez-Leija,Emmanuel Rivera-López,Oswaldo Hernández-González,Daniel Zavala-Reyes,Mariana Haydee García-Hernández,Juan Manuel Vargas-Morales,Rosa Del Carmen Milán-Segovia,Diana Patricia Portales-Pérez

Cancer cell 42:444-463.e10 PubMed38428410

2024

Multi-omic profiling of follicular lymphoma reveals changes in tissue architecture and enhanced stromal remodeling in high-risk patients.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea J Radtke,Ekaterina Postovalova,Arina Varlamova,Alexander Bagaev,Maria Sorokina,Olga Kudryashova,Mark Meerson,Margarita Polyakova,Ilia Galkin,Viktor Svekolkin,Sergey Isaev,Daniil Wiebe,Anna Sharun,Alexander Sarachakov,Grigory Perelman,Yaroslav Lozinsky,Ziv Yaniv,Bradley C Lowekamp,Emily Speranza,Li Yao,Stefania Pittaluga,Arthur L Shaffer,Danny Jonigk,James D Phelan,Theresa Davies-Hill,Da Wei Huang,Pavel Ovcharov,Krystle Nomie,Ekaterina Nuzhdina,Nikita Kotlov,Ravshan Ataullakhanov,Nathan Fowler,Michael Kelly,Jagan Muppidi,Jeremy L Davis,Jonathan M Hernandez,Wyndham H Wilson,Elaine S Jaffe,Louis M Staudt,Mark Roschewski,Ronald N Germain

Frontiers in immunology 14:1291643 PubMed38090569

2023

Changes in the immune landscape of TNBC after neoadjuvant chemotherapy: correlation with relapse.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed Ridha Moamin,Richard Allen,Steven Leslie Woods,Janet Elizabeth Brown,Harry Nunns,Anna Juncker-Jensen,Claire Elizabeth Lewis

Frontiers in immunology 14:1194087 PubMed37426665

2023

TMEM123 a key player in immune surveillance of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Pesce,Chiara Cordiglieri,Mauro Bombaci,Serenella Eppenberger-Castori,Stefania Oliveto,Cristina Manara,Mariacristina Crosti,Caner Ercan,Mairene Coto,Andrea Gobbini,Susanna Campagnoli,Tiziano Donnarumma,Manuele Martinelli,Valeria Bevilacqua,Elisa De Camilli,Paola Gruarin,Maria L Sarnicola,Elisa Cassinotti,Ludovica Baldari,Giuseppe Viale,Stefano Biffo,Sergio Abrignani,Luigi M Terracciano,Renata Grifantini

Cancer immunology, immunotherapy : CII 72:1169-1181 PubMed36357599

2022

Combined pembrolizumab and bevacizumab therapy effectively inhibits non-small-cell lung cancer growth and prevents postoperative recurrence and metastasis in humanized mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyun Qiao,Jinbo Zhao,Xiangbing Xin,Yanlu Xiong,Wenwen Guo,Fancheng Meng,Hui Li,Yangbo Feng,Hui Xu,Changhong Shi,Yong Han

Nature immunology 23:1256-1272 PubMed35902638

2022

Partial RAG deficiency in humans induces dysregulated peripheral lymphocyte development and humoral tolerance defect with accumulation of T-bet B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Krisztian Csomos,Boglarka Ujhazi,Peter Blazso,Jose L Herrera,Christopher M Tipton,Tomoki Kawai,Sumai Gordon,Maryssa Ellison,Kevin Wu,Matthew Stowell,Lauren Haynes,Rachel Cruz,Bence Zakota,Johnny Nguyen,Michelle Altrich,Christoph B Geier,Svetlana Sharapova,Joseph F Dasso,Jennifer W Leiding,Grace Smith,Waleed Al-Herz,Mayra de Barros Dorna,Olajumoke Fadugba,Eva Fronkova,Veronika Kanderova,Michael Svaton,Sarah E Henrickson,Joseph D Hernandez,Taco Kuijpers,Snezhina Mihailova Kandilarova,Elizaveta Naumova,Tomas Milota,Anna Sediva,Despina Moshous,Benedicte Neven,Tara Saco,Ravishankar Sargur,Sinisa Savic,John Sleasman,Gauri Sunkersett,Brant R Ward,Masanobu Komatsu,Stefania Pittaluga,Attila Kumanovics,Manish J Butte,Michael P Cancro,Shiv Pillai,Eric Meffre,Luigi D Notarangelo,Jolan E Walter

Clinical cancer research : an official journal of 25:6511-6523 PubMed31375512

2019

Genetic Aberrations in the CDK4 Pathway Are Associated with Innate Resistance to PD-1 Blockade in Chinese Patients with Non-Cutaneous Melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Yu,Junya Yan,Qian Guo,Zhihong Chi,Bixia Tang,Bin Zheng,Jinyu Yu,Ting Yin,Zhiyuan Cheng,Xiaowen Wu,Huan Yu,Jie Dai,Xinan Sheng,Lu Si,Chuanliang Cui,Xue Bai,Lili Mao,Bin Lian,Xuan Wang,Xieqia Yan,Siming Li,Li Zhou,Keith T Flaherty,Jun Guo,Yan Kong
View all publications
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