AB197730

Alexa Fluor® 488 Anti-CD45 antibody [F10-89-4]

Name: Anti-CD45 Alexa Fluor® 488 antibody [F10-89-4] (ab197730) IgG2a | Abcam
Brand: Abcam Limited
SKU: AB197730

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(6 Publications)

Mouse Monoclonal CD45 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD45 antibody [F10-89-4] (AB197730)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD45 antibody [F10-89-4] (AB197730)

ab197730 staining CD45 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197730 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in Jurkat cells fixed with 80% methanol (5 min).

Unconjugated

Anti-CD45 antibody [F10-89-4] - Hematopoietic Stem Cell Marker
Carrier free

Anti-CD45 antibody [F10-89-4] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

F10-89-4

Isotype

IgG2a

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde (10 min) or 80% methanol (5 min).</p>" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1640500 PubMed40873566

2025

Patient-derived colorectal microtumors predict response to anti-PD-1 therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Duy T Nguyen,Matthew A Schaller,Krista P Terracina,Xia Xu,Diego I Pedro,Alfonso Pepe,Juan M Urueña,Zadia Dupee,Nickolas Diodati,Ryan A Smolchek,Jack E Famiglietti,Nhi Tran Yen Nguyen,Gerik W Tushoski-Alemán,Kuoyuan Cheng,Lan Chen,Doug Linn,Vania Vidimar,Aquila Fatima,Soon Woo Kwon,Dongyu Sun,Hongmin Chen,Haiyan Xu,Brian Long,Lily Y Moy,Bonnie J Howell,George H Addona,W Gregory Sawyer

Frontiers in immunology 13:903882 PubMed35711455

2022

Tumor-Derived Exosomes Regulate Apoptosis of CD45EpCAM Cells in Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shixiang Lu,Zhen Sun,Lili Liu,Peng Li,Bin Li,Wenjing Li,Zhaojun Wu,Mingming Zhao,Wenna Liu,Yongjie Wang,Bin Wang

Stem cells international 2020:2803747 PubMed32377202

2020

Enhanced Effect of IL-1-Activated Adipose-Derived MSCs (ADMSCs) on Repair of Intestinal Ischemia-Reperfusion Injury via COX-2-PGE Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Liu,Yi Ren He,Shao Jun Liu,Lei Hu,Li Chuang Liang,Dong Liang Liu,Lin Liu,Zhi Qiang Zhu

EBioMedicine 41:236-243 PubMed30872130

2019

Value of folate receptor-positive circulating tumour cells in the clinical management of indeterminate lung nodules: A non-invasive biomarker for predicting malignancy and tumour invasiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Qianjun Zhou,Qing Geng,Lin Wang,Jia Huang,Meilin Liao,Yan Li,Zhengping Ding,Shentu Yang,Hang Zhao,Qiang Shen,Changqing Pan,Jiatao Lou,Shun Lu,Chang Chen,Qingquan Luo

Scientific reports 8:1188 PubMed29352248

2018

Combined use of EpCAM and FRα enables the high-efficiency capture of circulating tumor cells in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Luojun Chen,Min Peng,Na Li,Qibin Song,Yi Yao,Bin Xu,Huali Liu,Peng Ruan

European journal of immunology 10:737-44 PubMed7428806

1980

Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat.

Applications

Unspecified application

Species

Unspecified reactive species

R Dalchau,J Kirkley,J W Fabre

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-CD45 antibody [F10-89-4]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD45 antibody [F10-89-4] (AB197730)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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