JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB256263

Alexa Fluor® 488 Anti-CD45RA antibody [MRC OX-33]

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal CD45 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Rat samples.

View Alternative Names

CD45, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA, Ptprc

1 Images
Flow Cytometry - Alexa Fluor® 488 Anti-CD45RA antibody [MRC OX-33] (AB256263)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 488 Anti-CD45RA antibody [MRC OX-33] (AB256263)

Lewis rat splenocytes stained with ab256263 (right) or mouse IgG1κ Alexa Fluor® 488 isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256263) or mouse IgG1κ Alexa Fluor® 488 isotype (1x 106 in 100 μl at 0.2 μg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MRC OX-33

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is directed against a high molecular weight band of the leucocyte common antigen (LCA).

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2500", "FlowCyt-species-notes": "<p></p>" } } }
style
left-column

You may be interested in:

AB277445

Mouse CD45 ELISA kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U myeloma cell line. This clone has been described reacting with paraffin embedded material following PLP fixation (see Whiteland et al., 1995). It only labels B cells among thoracic duct lymphocytes, with little labelling in bone marrow and none on thymocytes (Barclay et al., 1987).

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45RA also known as CD45RA antigen and T200 is an isoform of the CD45 protein family. It is a transmembrane protein tyrosine phosphatase with a molecular mass of approximately 220 kDa. CD45RA expression is abundant on naive T-cells and subsets of other immune cells like B cells. Researchers frequently use CD45RA antibodies including CD45RA-PE and CD45RA-FITC for flow cytometry to identify and study immune cell subsets.
Biological function summary

CD45RA plays a significant role in regulating T-cell receptor (TCR) signaling. By dephosphorylating specific phosphotyrosine residues on substrates CD45RA modulates signal transduction pathways important for T-cell activation and differentiation. This protein does not act alone but is part of a regulatory network that influences immune responses and cellular development through its interaction with other cell surface receptors and intracellular kinases.

Pathways

CD45RA modulation is integral to T-cell development and activation processes. It is an important participant in the T-cell receptor signaling pathway and the Jak-STAT signaling pathway. In these pathways CD45 interacts with proteins such as Lck and Fyn which are essential for propagating receptor-mediated signaling events in T-cells. Effective signal regulation through CD45RA is necessary to ensure appropriate immune responses.

CD45RA has links to autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The aberrant expression or function of CD45RA on immune cells can lead to improper immune activation or suppression contributing to the pathology of these diseases. Crossover with other proteins such as CD45RO which is an isoform involved in memory T-cell function indicates a broader network of immune regulation where CD45RA maintains the balance between immune activation and tolerance.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
See full target information Ptprc
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB212774

Anti-CD45RA antibody [PTPRC/1148] - BSA and Azide free

primary-antibodies

cd45ra-antibody-ptprc-1148-bsa-and-azide-free-ab212774

0

(0 reviews)

Alternative Product
Primary Antibodies

AB8217

Anti-CD45RA antibody [MEM-56]

primary-antibodies

cd45ra-antibody-mem-56-ab8217

0

(0 reviews)

Alternative Product
Primary Antibodies

AB18240

FITC Anti-CD45RA antibody [MEM-56]

primary-antibodies

fitc-cd45ra-antibody-mem-56-ab18240

0

(0 reviews)

Alternative Product
Primary Antibodies

AB239324

PE/DyLight™ 594 Anti-CD45RA antibody [MEM-56]

primary-antibodies

pe-dylight-594-cd45ra-antibody-mem-56-ab239324

0

(0 reviews)

Alternative Product
Primary Antibodies

AB155362

PerCP/Cy5.5® Anti-CD45RA antibody [HI100]

primary-antibodies

percp-cy55-cd45ra-antibody-hi100-ab155362

4

(1 reviews)

Alternative Product
Primary Antibodies

AB289799

Anti-CD45RA antibody [SPM504] - BSA and Azide free

primary-antibodies

cd45ra-antibody-spm504-bsa-and-azide-free-ab289799

0

(0 reviews)

Alternative Product
Primary Antibodies

AB231426

Anti-CD45RA antibody [SPM504]

primary-antibodies

cd45ra-antibody-spm504-ab231426

4

(1 reviews)

Alternative Product
Primary Antibodies

AB218501

Anti-CD45RA antibody [PTPRC/1148]

primary-antibodies

cd45ra-antibody-ptprc-1148-ab218501

0

(0 reviews)

Alternative Product
Primary Antibodies

AB239312

PE/Cy7® Anti-CD45RA antibody [MEM-56]

primary-antibodies

pe-cy7-cd45ra-antibody-mem-56-ab239312

0

(0 reviews)

Alternative Product
Primary Antibodies

AB59168

Anti-CD45RA antibody [F8-11-13]

primary-antibodies

cd45ra-antibody-f8-11-13-ab59168

0

(0 reviews)

Alternative Product
Primary Antibodies

AB218648

redFluor™ 710 Anti-CD45RA antibody [HI100]

primary-antibodies

redfluor-710-cd45ra-antibody-hi100-ab218648

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com