Alexa Fluor® 488 Anti-CD63 antibody [KILL150A]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD63 antibody [KILL150A] (AB318246)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling CD63 with ab318246 at 1/50 (10.0 ug/ml) dilution.

Negative control : no staining on human breast.
The section was incubated with ab318246 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• Flow Cyt

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Flow Cytometry - Alexa Fluor® 488 Anti-CD63 antibody [KILL150A] (AB318246)

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD63 with ab318246 at 1/500 dilution (0.1ug) / Right compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.
Cells are co-stained with CD14 conjugated to Alexa Fluor® 647.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD63 antibody [KILL150A] (AB318246)

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling CD63 with ab318246 at 1/50 (10.0 ug/ml) dilution.

Cytoplasmic staining on human melanoma.
The section was incubated with ab318246 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-CD63 antibody [KILL150A] (AB318246)

Flow cytometric analysis of SK-MEL-28 (Human malignant melanoma) cells labelling CD63 with ab318246 at 1/500 dilution (0.1ug) (Red) compared with a Mouse IgG1 [MOPC-21] - Isotype Control (AlexaFluor® 488) (ab234075) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Gated on viable cells.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD63 antibody [KILL150A] (AB318246)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling CD63 with ab318246 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in HUVEC cell line(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CD63 antibody [KILL150A] (AB318246)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-MEL-28 (human malignant melanoma cell) cells labelling CD63 with ab318246 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in SK-MEL-28 cell line(shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).