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AB288659

Alexa Fluor® 488 Anti-CD72 antibody [3F3]

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Mouse Monoclonal CD72 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD72 protein.

View Alternative Names

CD72, B-cell differentiation antigen CD72, Lyb-2

2 Images
Flow Cytometry - Alexa Fluor® 488 Anti-CD72 antibody [3F3] (AB288659)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-CD72 antibody [3F3] (AB288659)

Separation of human CD72 positive lymphocytes (red-filled) from CD72 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using ab288659 (4 μl reagent / 100 μl of peripheral whole blood).

Flow Cytometry - Alexa Fluor® 488 Anti-CD72 antibody [3F3] (AB288659)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-CD72 antibody [3F3] (AB288659)

Flow cytometry surface staining pattern of human peripheral whole blood stained using ab288659 (4 μl reagent / 100 μl of peripheral whole blood).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3F3

Isotype

IgG2b

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD72 protein. The exact immunogen used to generate this antibody is proprietary information.

P21854

Specificity

The mouse monoclonal antibody 3F3 recognizes an extracellular epitope of CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD72 also known as Lyb-2 is a membrane protein with a molecular weight of approximately 45-50 kDa. It is part of the C-type lectin family and expresses mainly on B cells. CD72 serves as a receptor and recognizes ligands to mediate cell signaling. The protein is also present in some myeloid cells contributing to its diverse roles in the immune system. The function of CD72 involves the modulation of B cell receptor (BCR) signaling which is important for B cell development and activation.
Biological function summary

CD72 functions as a regulatory protein that influences B cell activity by modulating BCR signaling thresholds. CD72 is part of receptor-ligand complexes on the B cell surface. It operates alongside other proteins like CD5 and SHP-1 to fine-tune immune responses. By interacting with these proteins CD72 helps in maintaining immune balance and prevents overactivation that can lead to autoimmune reactions. This protein modulates both positive and negative signaling affecting B cell survival and differentiation.

Pathways

CD72 influences immune response pathways specifically the BCR signaling pathway and the downstream PI3K-Akt pathway. As a negative regulator CD72 works in tandem with proteins such as Lyn and SHP-1 to attenuate BCR signal transduction. These signaling cascades play a role in immune responses lymphocyte development and adaptation. The involvement of CD72 in these pathways illustrates its function in balancing the intensity of signals received by B cells ultimately affecting immune response outcomes.

CD72 dysregulation has been linked to systemic lupus erythematosus (SLE) and certain lymphomas. Abnormal expression or function of CD72 can lead to improper B cell activation contributing to autoimmune conditions like SLE. In these diseases CD72 interaction with proteins such as SHP-1 and CD22 becomes disrupted affecting immune tolerance and promoting pathological immune responses. Additionally altered CD72 expression or mutations have been observed in B cell malignancies indicating its role in the pathology of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Co-receptor of B cell receptor (BCR) that plays both positive and negative roles on B-cell functions. Recognizes the Sm/ribonucleoprotein (RNP) self-antigen ligand, and coligation of CD72 and BCR inhibits BCR signaling. Mechanistically, ligand binding leads to the recruitment of PTPN6/SHP-1 to the BCR complex which is inhibitory to BCR signaling. Also acts as a ligand for CD5 and thereby plays a critical role in maintaining regulatory T and B-cell homeostasis.
See full target information CD72
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Product promise

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