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AB256296

Alexa Fluor® 488 Anti-CD8 alpha antibody [OX-8]

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(3 Publications)

Mouse Monoclonal CD8 alpha antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Rat samples. Cited in 3 publications.

View Alternative Names

CD8a, MAL, CD8A, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

1 Images
Flow Cytometry - Alexa Fluor® 488 Anti-CD8 alpha antibody [OX-8] (AB256296)
  • Flow Cyt

Unknown

Flow Cytometry - Alexa Fluor® 488 Anti-CD8 alpha antibody [OX-8] (AB256296)

Lewis rat splenocytes stained with ab256296 (right) or mouse IgG1κ Alexa Fluor® 488 isotype (left). Lewis rat splenocytes were incubated for 30 min on ice in 1x PBS / 10 % rat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab256296) or mouse IgG1κ Alexa Fluor® 488 isotype (1x 106 in 100 μl at 0.2 μg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD3.

Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.

  • 660 APC

    APC Anti-CD8 alpha antibody [OX-8]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX-8

Isotype

IgG1

Light chain type

kappa

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Rat, Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/2500", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD8 alpha also known as CD8A or CD8 protein is a glycoprotein subunit expressed on the surface of cytotoxic T lymphocytes. It has a mass of approximately 32 kDa. Found on the surface cell membrane CD8 alpha functions primarily in the immune response specifically in the recognition of antigens bound to major histocompatibility complex (MHC) class I molecules. Often scientists use CD8 antibodies for detection and CD8 IHC or immunohistochemistry for localization studies.
Biological function summary

The CD8 alpha protein plays a critical role in T-cell mediated immune responses. It forms a heterodimer with the CD8 beta chain creating the CD8 alpha-beta complex that strengthens T-cell interaction with antigen-presenting cells. CD8 alpha also helps in signaling processes that activate T cells equipping them to destroy infected or malignant cells. Researchers often study CD8 alpha peptides to understand its interactions better.

Pathways

CD8 alpha is integral to the T-cell receptor signaling pathway and the cytotoxic T lymphocyte (CTL) pathway. The T-cell receptor complex which includes the CD8 molecule transmits signals that are important for T-cell activation and function. CD8 interacts with key proteins such as the T-cell receptor (TCR) and MHC class I molecules facilitating targeted responses against pathogens. These pathways highlight CD8 alpha’s role in adaptive immunity.

CD8 alpha is most prominently associated with viral infections and cancer. Conditions like HIV and some forms of leukemia show altered CD8 function highlighting the protein's role in immune surveillance. In HIV infection for instance CD8 T cells reduce in number impairing the immune response. CD8 alpha’s connection to the immune system places it alongside other immune proteins such as CD4 and MHC molecules in the context of immune dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by the gene CD8A is an integral membrane glycoprotein crucial for immune responses, operating mainly in T-cells as a coreceptor for MHC class I molecule : peptide complexes. These peptides originate from cytosolic proteins, unlike class II peptides, which are from extracellular proteins. CD8A interacts with the T-cell receptor (TCR) and MHC class I proteins on antigen-presenting cells, recruiting the Src kinase LCK to the TCR-CD3 complex. LCK phosphorylates substrates, triggering signaling pathways that lead to the production of lymphokines, enhanced motility, adhesion, and activation of cytotoxic T-lymphocytes (CTLs), thereby aiding in the recognition and elimination of infected or tumor cells. Additionally, in natural killer (NK) cells, CD8A homodimers on the cell surface provide a survival mechanism for conjugating with and lysing multiple target cells. CD8A homodimers also facilitate the survival and differentiation of activated lymphocytes into memory CD8 T-cells. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CD8A
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:97125 PubMed39958562

2025

Landscape of four different stages of human gastric cancer revealed by single-cell sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Xu-Shan Tang,Chun-Lei Xu,Na Li,Jian-Qing Zhang,Yong Tang

Cell death & disease 15:573 PubMed39117605

2024

Elucidating the role of S100A10 in CD8 T cell exhaustion and HCC immune escape via the cPLA2 and 5-LOX axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ganggang Wang,Xiaowei Shen,Wenzhi Jin,Chao Song,Meiyuan Dong,Zhijie Zhou,Xiaoliang Wang

Nature cancer 3:337-354 PubMed35256819

2022

An anti-PD-1-GITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Chan,Nicole Belmar,Sun Ho,Bryan Rogers,Marcia Stickler,Michelle Graham,Eileen Lee,Ninian Tran,Dong Zhang,Priyanka Gupta,Mien Sho,Tracy MacDonough,Andrew Woolley,Han Kim,Hong Zhang,Wei Liu,Pingping Zheng,Zoltan Dezso,Kyle Halliwill,Michele Ceccarelli,Susan Rhodes,Archana Thakur,Charles M Forsyth,Mengli Xiong,Siu Sze Tan,Ramesh Iyer,Marc Lake,Enrico Digiammarino,Li Zhou,Lance Bigelow,Kenton Longenecker,Russell A Judge,Cassie Liu,Max Trumble,Jonathan P Remis,Melvin Fox,Belinda Cairns,Yoshiko Akamatsu,Diane Hollenbaugh,Fiona Harding,Hamsell M Alvarez
View all publications
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