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Rabbit Monoclonal CDX2 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (AB195007), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (AB195007), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (AB195007), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (AB195007), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (AB195007), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Rabbit
Predicted
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rabbit
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Rabbit
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Rabbit
Dilution info
-
Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

2 products for Alternative Version

Target data

Function

Transcription factor which regulates the transcription of multiple genes expressed in the intestinal epithelium (By similarity). Binds to the promoter of the intestinal sucrase-isomaltase SI and activates SI transcription (By similarity). Binds to the DNA sequence 5'-ATAAAAACTTAT-3' in the promoter region of VDR and activates VDR transcription (By similarity). Binds to and activates transcription of LPH (By similarity). Activates transcription of CLDN2 and intestinal mucin MUC2 (By similarity). Binds to the 5'-AATTTTTTACAACACCT-3' DNA sequence in the promoter region of CA1 and activates CA1 transcription (By similarity). Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Binds preferentially to methylated DNA (PubMed:28473536).

Alternative names

CDX3, CDX2, Homeobox protein CDX-2, CDX-3, Caudal-type homeobox protein 2

Recommended products

Rabbit Monoclonal CDX2 antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2764Y
Purification technique
Affinity purification Protein A
Dissociation constant
1 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CDX2 or Caudal Type Homeobox 2 is a transcription factor involved in intestinal development and differentiation. Alternative names for CDX2 include Cdx-2 and CDX-2. This protein has a molecular mass of approximately 38 kDa. CDX2 is expressed mainly in the epithelial cells of the intestine. Researchers can identify CDX2 through techniques like CDX2 stain CDX2 immunostain and CDX2 IHC which are useful for determining its expression in tissue samples.

Biological function summary

CDX2 plays an important role in controlling the expression of genes necessary for maintaining intestinal epithelial cell identity. It functions as a part of regulatory networks that guide cell fate and differentiation in the gut. CDX2 does not generally form complexes but acts as a transcriptional regulator that binds directly to DNA influencing genes involved in cell adhesion proliferation and the maintenance of tissue structure.

Pathways

CDX2 is integral to the Wnt signaling and Hedgehog pathways which are vital for intestinal development and homeostasis. Within these pathways CDX2 works closely with other proteins like beta-catenin influencing cell proliferation and differentiation. The Wnt pathway in particular modulates the expression of proliferative genes while CDX2 ensures that proliferation maintains tissue integrity by guiding cellular differentiation.

Associated diseases and disorders

CDX2 has a significant link to colorectal cancer and Barrett's esophagus. Loss of CDX2 expression often marks the progression from normal to malignant tissue in colorectal cancer where it connects with proteins like p-catenin affecting cellular growth and differentiation. Similarly abnormal expression of CDX2 correlates with the development of Barrett's esophagus a condition that may progress to esophageal adenocarcinoma. These disease associations make CDX2 a potential diagnostic marker and therapeutic target in gastrointestinal pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

    Immunohistochemistry analysis of paraffin-embedded mouse cardiac muscle tissue sections labeling CDX2 with ab195007 at 1/100 dilution, incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, Epitope Retrieval Solution2) for 40 mins. 

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Negative control: no staining on mouse cardiac muscle.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

    Immunohistochemistry analysis of paraffin-embedded rat colon tissue sections labeling CDX2 with ab195007 at 1/100 dilution, incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, Epitope Retrieval Solution2) for 40 mins. 

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Nuclear staining on rat colon.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

    Immunohistochemistry analysis of paraffin-embedded mouse colon tissue sections labeling CDX2 with ab195007 at 1/100 dilution, incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, Epitope Retrieval Solution2) for 40 mins. 

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Nuclear staining on mouse colon.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

    Immunofluorescence showing negative staining of CDX2 in a section of formalin-fixed paraffin-embedded human skeletal muscle.

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab195007 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (ab195007)

    Immunofluorescence staining of CDX2 in a section of formalin-fixed paraffin-embedded human normal colon.

    Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab195007 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com