Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (AB195007)

Immunofluorescence staining of CDX2 in a section of formalin-fixed paraffin-embedded human normal colon.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab195007 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (AB195007)

Immunofluorescence showing negative staining of CDX2 in a section of formalin-fixed paraffin-embedded human skeletal muscle.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0) using retrieval settings of 110°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab195007 at 1/100 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (AB195007)

Immunohistochemistry analysis of paraffin-embedded mouse cardiac muscle tissue sections labeling CDX2 with ab195007 at 1/100 dilution, incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, Epitope Retrieval Solution2) for 40 mins.  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Negative control : no staining on mouse cardiac muscle.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (AB195007)

Immunohistochemistry analysis of paraffin-embedded rat colon tissue sections labeling CDX2 with ab195007 at 1/100 dilution, incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, Epitope Retrieval Solution2) for 40 mins.  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Nuclear staining on rat colon.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CDX2 antibody [EPR2764Y] (AB195007)

Immunohistochemistry analysis of paraffin-embedded mouse colon tissue sections labeling CDX2 with ab195007 at 1/100 dilution, incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH 9.0, Epitope Retrieval Solution2) for 40 mins.  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Nuclear staining on mouse colon.