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Rabbit Recombinant Monoclonal CGRP-I antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (AB305115), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (AB305115), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (AB305115), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Expected
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

    Target data

    Function

    CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.

    Alternative names

    Calcitonin gene-related peptide 1, Alpha-type CGRP, Calcitonin gene-related peptide I, CGRP-I, CALCA, CALC1

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    Rabbit Recombinant Monoclonal CGRP-I antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples.

    Alternative names

    Calcitonin gene-related peptide 1, Alpha-type CGRP, Calcitonin gene-related peptide I, CGRP-I, CALCA, CALC1

    Key facts

    Isotype

    IgG

    Conjugation

    Alexa Fluor® 488

    Excitation/Emission

    Ex: 495nm, Em: 519nm

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Clone number

    EPR23804-95

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate short-term storage duration

    1-2 weeks

    Appropriate short-term storage conditions

    +4°C

    Appropriate long-term storage conditions

    -20°C

    Aliquoting information

    Upon delivery aliquot

    Storage information

    Avoid freeze / thaw cycle, Store in the dark

    Notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    Supplementary info

    Activity summary

    The target designated as CGRP-I and CGRP-II refers to calcitonin gene-related peptide isoforms which play key roles in various physiological functions. CGRP around 37 amino acids long has alternative names such as alpha-CGRP and beta-CGRP occurring mainly in the central nervous system and peripheral nervous tissues. These peptides are potent vasodilators integral to the modulation of blood vessel tone and immune responses. Both isoforms though small in mass allow for significant physiological impacts due to their receptor interactions.

    Biological function summary

    CGRP-I and CGRP-II contribute to vasodilation and pain transmission in the nervous system. Each isoform can activate specific CGRP receptors mainly located on smooth muscle cells and neurons without forming stable complexes. Their activity involves increasing intracellular cAMP levels facilitating vascular relaxation and modulating nociceptive pathways. Consequently CGRP peptides are essential for conveying pain signals and supporting neurogenic inflammation mechanisms.

    Pathways

    CGRP-I and CGRP-II serve critical roles in the cardiovascular and nervous system pathways. The cAMP-dependent pathway one of the essential routes of action elevates vasodilation responses where CGRP interacts with the RAMP1 protein a receptor accessory protein. Another pathway involves the modulation of pain where CGRP interacts with other neurotransmitters and proteins such as substance P and neurokinin.

    Associated diseases and disorders

    CGRP-I and CGRP-II show significant associations with migraines and hypertension. These peptides through their vasodilatory capabilities and nociceptive signaling connect to migraines by interacting with proteins like RAMP1 in trigeminal neurons exacerbating migraine pathology. Furthermore CGRP's role in promoting vasodilation links to hypertension management where altered CGRP activity affects blood pressure regulation. These connections make CGRP-related pathways viable targets for therapeutic intervention.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    3 product images

    • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab305115), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab305115)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TT (human thyroid carcinoma epithelial cell) cells labelling CGRP-I+CGRP-II with ab305115 at 1/50 (10.0 ug/ml) dilution. Confocal image showing cytoplasmic staining in TT cells. Negative control: 293T (PMID: 32296769). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: PBS instead of the primary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab305115), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab305115)

      Immunohistochemical analysis of paraffin-embedded Rat spinal cord tissue labeling CGRP-I+CGRP-II with ab305115 at 1/100 (5.0 ug/ml) followed by a Ready to use dilution. Positive staining on rat spinal cord.The section was incubated with ab305115 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Secondary antibody only control: Secondary antibody is at Ready to use dilution.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins. Negative control: PBS instead of the primary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab305115), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CGRP-I+CGRP-II antibody [EPR23804-95] (ab305115)

      Immunohistochemical analysis of paraffin-embedded Mouse spinal cord tissue labeling CGRP-I+CGRP-II with ab305115 at 1/100 (5.0 ug/ml) followed by a at Ready to use dilution. Positive staining on mouse spinal cord.The section was incubated with ab305115 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      Secondary antibody only control: Secondary antibody is at Ready to use dilution.

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 40 mins. Negative control: PBS instead of the primary antibody.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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