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AB309248

Alexa Fluor® 488 Anti-Collagen I antibody [EPR24331-53]

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(1 Publication)

Anti-Collagen I antibody [EPR24331-53] - Alexa Fluor® 488 conjugated (ab309248) is a rabbit recombinant monoclonal antibody detecting Collagen I in IHC-P, ICC/IF. Suitable for Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Cola1, Col1a1, Collagen alpha-1(I) chain, Alpha-1 type I collagen

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Collagen I antibody [EPR24331-53] (AB309248)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Collagen I antibody [EPR24331-53] (AB309248)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling Collagen I with ab309248 at 1/100 (5.0 µg/mL). Cytoplasmic staining on rat testis. The section was incubated with ab309248 for 60 mins at room temperature (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Collagen I antibody [EPR24331-53] (AB309248)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Collagen I antibody [EPR24331-53] (AB309248)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling Collagen I with ab309248 at 1/100 (5.0 µg/mL). Cytoplasmic staining on mouse testis. The section was incubated with ab309248 for 60 mins at room temperature (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Collagen I antibody [EPR24331-53] (AB309248)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Collagen I antibody [EPR24331-53] (AB309248)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling Collagen I with ab309248 at 1/250 (2.0 ug/ml) dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cells. Negative control : Hepa1-6 (PMID : 27853186). The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Collagen I antibody [EPR24331-53]

  • 660 APC

    APC Anti-Collagen I antibody [EPR24331-53]

  • 578 PE

    PE Anti-Collagen I antibody [EPR24331-53]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Collagen I antibody [EPR24331-53]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Collagen I antibody [EPR24331-53]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Collagen I antibody [EPR24331-53]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Collagen I antibody [EPR24331-53]

  • Unconjugated

    Anti-Collagen I antibody [EPR24331-53]

  • Carrier free

    Anti-Collagen I antibody [EPR24331-53] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24331-53

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-Collagen I antibody [EPR24331-53] (ab309248) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse, Rat samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Related products
Antibody clone EPR24331-53 is also available pre-conjugated to a variety of labels for your convenience – Anti-Collagen I Alexa Fluor® 488 [EPR24331-53] (ab309248).

Other related products
We have a range of other formats of antibody clone [EPR24331-53] also available for your convenience: ab270993, Carrier free - ab279711, Alexa Fluor® 488 - ab309248, Alexa Fluor® 647 - ab309367, APC - ab310848, PE - ab310918, Alexa Fluor® 568 - ab312943, Alexa Fluor® 555 - ab313153, Alexa Fluor® 594 - ab313351, Alexa Fluor® 750 - ab321535

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Collagen type I also called collagen I is a structural protein expressed mainly in connective tissues such as skin tendon bone and ligaments. It serves as an important component in providing mechanical strength and integrity to these tissues. Collagen I is a fibrillar collagen known for its triple-helix structure composed of two alpha-1 chains and one alpha-2 chain and has a molecular mass of approximately 300 kDa. Researchers often employ collagen western blot and collagen ELISA techniques for its detection. Collagen suppliers offer various collagen antibodies used in these assays to study its distribution and function.
Biological function summary

Collagen type I plays a central role in maintaining the extracellular matrix and supporting cellular environments. It interacts with other matrix proteins and cells forming complexes that help in tissue development and repair. Type I collagen is especially important in bone matrix working alongside minerals like hydroxyapatite to provide rigidity and support. Anti-collagen antibodies aid in studying its biological functions and interactions which are critical to understanding tissue dynamics.

Pathways

Collagen type I interacts with multiple signaling cascades involved in tissue remodeling and repair. It is a significant player in the TGF-Β pathway which regulates fibrosis and wound healing processes. In these pathways proteins such as fibronectin and integrins work in concert with collagen type I to orchestrate cellular responses to damage. Researchers often examine its role in these pathways to uncover therapeutic possibilities for disease interventions.

Collagen type I has strong connections to conditions like osteogenesis imperfecta and fibrosis. Mutations or irregularities in collagen I production can lead to osteogenesis imperfecta a genetic disorder characterized by brittle bones. In fibrosis excessive collagen deposition disrupts normal tissue architecture contributing to organ dysfunction. In both conditions type I collagen interacts with other proteins like matrix metalloproteinases which modulate its breakdown and remodeling highlighting its importance in disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information Col1a1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 30:708 PubMed40765006

2025

Adipose tissue-associated Krt23+fibroblasts contribute to immune microenvironment disorders in mouse adipose tissue during the development of obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Lu,Ling Ding,Sen Zhang,Xing Jiang,Wen Ma,Youhan Liu,Xuewen Tian,Qinglu Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com