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AB198590

Alexa Fluor® 488 Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker

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(1 Publication)

Mouse Monoclonal COX IV antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, COX4L2, COX4I2, Cytochrome c oxidase subunit IV isoform 2, COX IV-2

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (AB198590)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker (AB198590)

ab198590 staining COX4+COX4L2 in WI38 cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198590 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 2μg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Unconjugated

    Anti-COX4 + COX4L2 antibody [10G8D12C12] - Mitochondrial Marker

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10G8D12C12

Isotype

IgG2a

Light chain type

kappa

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB239711

Cytochrome C Oxidase Assay Kit

Functional Studies - Cytochrome C Oxidase Assay Kit (AB239711)

  • 0
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Primary Antibodies

AB14705

Anti-MTCO1 antibody [1D6E1A8]

BOND RX™ Validated|Recombinant

Western blot - Anti-MTCO1 antibody [1D6E1A8] (AB14705)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB14734

Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control

Recombinant|Lab Essentials|KO Validated

Western blot - Anti-VDAC1/Porin + VDAC3 antibody [20B12AF2] - Mitochondrial Loading Control (AB14734)

  • 5
  • 52
Primary Antibodies

AB40742

HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control

BOND RX™ Validated

Western blot - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (AB40742)

  • 5
  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
ab198590 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. ab198590 was judged as near homogeneity by SDS PAGE.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The COX4 protein also known as Cytochrome c oxidase subunit 4 along with its isoform COX4L2 forms part of the electron transport chain in mitochondria. COX4 has a molecular mass of approximately 20.8 kDa and is found in various tissues especially in high-energy-demand organs like the heart and liver. Both COX4 and COX4L2 belong to the inner mitochondrial membrane and play a role in the enzyme complex Cytochrome c oxidase essential for efficient cellular respiration.
Biological function summary

COX4 and COX4L2 contribute to the terminal stage of the mitochondrial electron transport chain. They form core components of the Cytochrome c oxidase complex also called complex IV which facilitates the transfer of electrons from reduced cytochrome c to oxygen. This process not only aids in the production of water but also helps create the proton gradient used by ATP synthase to generate ATP the primary energy currency in cells.

Pathways

Both COX4 and COX4L2 integrate into key metabolic pathways such as oxidative phosphorylation and the respiratory chain. Their role in the electron transport chain connects them to other mitochondrial proteins like cytochrome c and ATP synthase. This collaboration is important for the efficient conversion of nutrients into usable cellular energy supporting essential metabolic functions.

Disruptions in the function of COX4 and COX4L2 relate to conditions such as mitochondrial myopathy and Leigh syndrome. Mutations or deficiencies in these proteins can impair electron transport and reduce ATP generation leading to muscle weakness or neurodegenerative symptoms. Cytochrome c and other components of the electron transport chain often exhibit related dysfunctions in these mitochondrial diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix.
See full target information COX4I1

Additional targets

COX4I2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 7: PubMed29985131

2018

PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading.

Applications

Unspecified application

Species

Unspecified reactive species

Gergely Rona,Domenico Roberti,Yandong Yin,Julia K Pagan,Harrison Homer,Elizabeth Sassani,Andras Zeke,Luca Busino,Eli Rothenberg,Michele Pagano
View all publications
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Product promise

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