AB171449

Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9]

Name: Alexa Fluor® 488 conjugated Anti-CPT1A antibody [8F6AE9] (ab171449) | Abcam
Brand: Abcam Limited
SKU: AB171449
Price: 370 USD
Availability: InStock
Rating: 5 (4 reviews)

KO Validated
What is this?

(4 Reviews)

(18 Publications)

Anti-CPT1A antibody [8F6AE9] - Alexa Fluor® 488 conjugated (ab171449) is a mouse monoclonal antibody detecting CPT1A in Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human.

- Clone 8F6AE9 is the most cited clone to CPT1A
- KO validated for confirmed specificity
- Over 10 publications

View Alternative Names

CPT1, CPT1A, CPT1-L, Carnitine palmitoyltransferase 1A, Succinyltransferase CPT1A, CPT I, CPTI-L

2 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (AB171449)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (AB171449)

ab171449 staining CPT1A in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab171449 at 1/1000 dilution (shown in green) and ab190573, Rabbit monoclonal to alpha Tubulin (Alexa Fluor^® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (AB171449)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (AB171449)

Flow cytometry overlay histogram showing wild-type HAP1 (green line) and CPT1A knockout HAP1 stained with ab171449 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab171449) (1x 106 in 100μl at 1.0 μg/ml (1/500)) for 30min at 22°C.

Isotype control antibody Alexa Fluor 488 Mouse IgG2b kappa [MPC-11] - Isotype Control was used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line, CPT1A knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

HRP

HRP Anti-CPT1A antibody [8F6AE9]
Unconjugated

Anti-CPT1A antibody [8F6AE9]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

8F6AE9

Isotype

IgG2b

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "5 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/unavailable/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-alexa-fluorreg-488-ab171465'>ab171465</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "5 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/unavailable/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-alexa-fluorreg-488-ab171465'>ab171465</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Trusted by the scientific community
Anti-CPT1A [8F6AE9] Alexa Fluor® 488 (ab171449) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.

Related products
Antibody clone 8F6AE9 is also available pre-conjugated to a variety of labels for your convenience – Anti-CPT1A Alexa Fluor® 488 [8F6AE9] (ab171449).

Specificity confirmed
The specificity of Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (ab171449) has been confirmed by Flow Cytometry testing in CPT1A Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [8F6AE9] also available for your convenience: ab128568, Alexa Fluor® 488 - ab171449, HRP - ab198494, Carrier free - ab300265

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Precipitation Ammonium Sulphate

Purification notes

Purity is near homogeneity as judged by SDS-PAGE. ab171449 was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by ammonium sulfate precipitation.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CPT1A also known as carnitine palmitoyltransferase 1A is a vital enzyme involved in the transport of long-chain fatty acids across the mitochondrial membrane. This protein facilitates the conversion of fatty acids into acyl-carnitine a process necessary for beta-oxidation within the mitochondria. The molecular weight of CPT1A is approximately 88 kDa. CPT1A predominantly expresses in liver cells where energy metabolism from fatty acids is important. Alternate names for this target include CPT 1 and CTP1A reflecting its central role in metabolic processes related to lipid utilization.

Biological function summary

CPT1A plays a vital role in energy production by facilitating mitochondrial fatty acid oxidation. It does not function as a solitary enzyme but often associates with other enzymes forming a multiprotein complex that includes acyl-CoA synthetase. This complex enables efficient fatty acid transport and oxidation converting stored fats into usable energy. As CPT1A primarily operates in the liver its activity significantly impacts overall lipid and energy homeostasis demonstrating its critical regulatory role.

Pathways

CPT1A is central to the fatty acid beta-oxidation pathway an important process for breaking down fatty acids to produce energy. This pathway also involves proteins such as CPT1B a related isoform present in muscle tissues. CPT1A's function is important in the liver's capacity to regulate energy balance and respond to metabolic demands. Additionally CPT1A interacts within the signaling pathway of AMPK (AMP-activated protein kinase) which further integrates it into broader metabolic regulation networks linking energy status to cellular function.

CPT1A is notably associated with metabolic syndromes and fatty liver disease. Mutations or deficiencies in CPT1A can lead to disorders like hepatic carnitine palmitoyltransferase 1A deficiency characterized by hypoketotic hypoglycemia and hepatomegaly. Furthermore its dysregulation can impact proteins such as ACC1 (acetyl-CoA carboxylase) in the context of metabolic syndrome. Research continues to explore the implications of CPT1A in non-alcoholic steatohepatitis highlighting its relevance in liver energy metabolism disorders and their pathophysiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion (PubMed : 11350182, PubMed : 14517221, PubMed : 16651524, PubMed : 9691089). Possesses also a lysine succinyltransferase activity that can regulate enzymatic activity of substrate proteins such as ENO1 and metabolism independent of its classical carnitine O-palmitoyltransferase activity (PubMed : 29425493). Plays an important role in hepatic triglyceride metabolism (By similarity). Plays also a role in inducible regulatory T-cell (iTreg) differentiation once activated by butyryl-CoA that antagonizes malonyl-CoA-mediated CPT1A repression (By similarity). Sustains the IFN-I response by recruiting ZDHCC4 to palmitoylate MAVS at the mitochondria leading to MAVS stabilization and activation (PubMed : 38016475). Promotes ROS-induced oxidative stress in liver injury via modulation of NFE2L2 and NLRP3-mediated signaling pathways (By similarity).

See full target information CPT1A

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Immunity 57:1923-1938.e7 PubMed38878769

2024

Fasting reshapes tissue-specific niches to improve NK cell-mediated anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca B Delconte,Mark Owyong,Endi K Santosa,Katja Srpan,Sam Sheppard,Tomi J McGuire,Aamna Abbasi,Carlos Diaz-Salazar,Jerold Chun,Inez Rogatsky,Katharine C Hsu,Stefan Jordan,Miriam Merad,Joseph C Sun

iScience 27:108666 PubMed38155778

2023

IL-15 boosts activated HBV core-specific CD8 progenitor cells via metabolic rebalancing in persistent HBV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Peña-Asensio,Henar Calvo-Sánchez,Joaquín Miquel,Eduardo Sanz-de-Villalobos,Alejandro González-Praetorius,Miguel Torralba,Juan-Ramón Larrubia

JCI insight 8: PubMed37166984

2023

T cell metabolic reprogramming in acute kidney injury and protection by glutamine blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Kyungho Lee,Elizabeth A Thompson,Sepideh Gharaie,Chirag H Patel,Johanna T Kurzhagen,Phillip M Pierorazio,Lois J Arend,Ajit G Thomas,Sanjeev Noel,Barbara S Slusher,Hamid Rabb

Journal of controlled release : official journal of the Controlled Release Society 358:13-26 PubMed37086952

2023

Tanshinone IIA enhances the therapeutic efficacy of mesenchymal stem cells derived exosomes in myocardial ischemia/reperfusion injury via up-regulating miR-223-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng Li,Ke Yang,Weilong Cao,Rui Guo,Zhihao Liu,Jing Zhang,Aodi Fan,Yuting Huang,Chuanrui Ma,Lan Li,Guanwei Fan

Nature communications 13:7272 PubMed36433992

2022

Alveolar macrophage metabolic programming via a C-type lectin receptor protects against lipo-toxicity and cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Scur,Ahmad Bakur Mahmoud,Sayanti Dey,Farah Abdalbarri,Iona Stylianides,Daniel Medina-Luna,Gayani S Gamage,Aaron Woblistin,Alexa N M Wilson,Haggag S Zein,Ashley Stueck,Andrew Wight,Oscar A Aguilar,Francesca Di Cara,Brendon D Parsons,Mir Munir A Rahim,James R Carlyle,Andrew P Makrigiannis

Cancers 14: PubMed35205709

2022

Early Neutrophilia Marked by Aerobic Glycolysis Sustains Host Metabolism and Delays Cancer Cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Michele Petruzzelli,Miriam Ferrer,Martijn J Schuijs,Sam O Kleeman,Nicholas Mourikis,Zoe Hall,David Perera,Shwethaa Raghunathan,Michele Vacca,Edoardo Gaude,Michael J Lukey,Duncan I Jodrell,Christian Frezza,Erwin F Wagner,Ashok R Venkitaraman,Timotheus Y F Halim,Tobias Janowitz

Cell reports 37:109883 PubMed34706232

2021

β2-adrenergic receptor signaling regulates metabolic pathways critical to myeloid-derived suppressor cell function within the TME.

Applications

Unspecified application

Species

Unspecified reactive species

Hemn Mohammadpour,Cameron R MacDonald,Philip L McCarthy,Scott I Abrams,Elizabeth A Repasky

iScience 24:103312 PubMed34765928

2021

Reduced mitochondrial respiration in T cells of patients with major depressive disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Gamradt,Helge Hasselmann,Aline Taenzer,Jelena Brasanac,Victoria Stiglbauer,Arne Sattler,Max Sajitz-Hermstein,Sylwia Kierszniowska,Caren Ramien,Jan Nowacki,Lea Mascarell-Maricic,Katja Wingenfeld,Dominique Piber,Andreas Ströhle,Katja Kotsch,Friedemann Paul,Christian Otte,Stefan M Gold

Frontiers in immunology 12:700374 PubMed34434191

2021

Distinct Bioenergetic Features of Human Invariant Natural Killer T Cells Enable Retained Functions in Nutrient-Deprived States.

Applications

Unspecified application

Species

Unspecified reactive species

Priya Khurana,Chakkapong Burudpakdee,Stephan A Grupp,Ulf H Beier,David M Barrett,Hamid Bassiri

Cell reports 34:108863 PubMed33691089

2021

Metabolic programs define dysfunctional immune responses in severe COVID-19 patients.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Thompson,Katherine Cascino,Alvaro A Ordonez,Weiqiang Zhou,Ajay Vaghasia,Anne Hamacher-Brady,Nathan R Brady,Im-Hong Sun,Rulin Wang,Avi Z Rosenberg,Michael Delannoy,Richard Rothman,Katherine Fenstermacher,Lauren Sauer,Kathyrn Shaw-Saliba,Evan M Bloch,Andrew D Redd,Aaron A R Tobian,Maureen Horton,Kellie Smith,Andrew Pekosz,Franco R D'Alessio,Srinivasan Yegnasubramanian,Hongkai Ji,Andrea L Cox,Jonathan D Powell

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (AB171449)

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CPT1A antibody [8F6AE9] (AB171449)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

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