JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB215239

Alexa Fluor® 488 Anti-CRISPR-Cas9 antibody [EPR18991]

5

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal CRISPR-Cas9 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Transfected cell lysate - Streptococcus pyogenes samples. Cited in 1 publication.

View Alternative Names

csn1, SPy_1046, cas9, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CRISPR-Cas9 antibody [EPR18991] (AB215239)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-CRISPR-Cas9 antibody [EPR18991] (AB215239)

ab215239 staining CRISPR-Cas9 in NIH3T3-Cas9 cells. The lower panels demonstrate that ab215239 does not cross react with un-transfected NIH3T3 cells.

The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab215239 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Unconjugated

    Anti-CRISPR-Cas9 antibody [EPR18991]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CRISPR-Cas9 antibody [EPR18991]

  • Carrier free

    Anti-CRISPR-Cas9 antibody [EPR18991] - BSA and Azide free

  • HRP

    HRP Anti-CRISPR-Cas9 antibody [EPR18991]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18991

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Streptococcus pyogenes": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate - Streptococcus pyogenes": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p>This product gave a positive signal in Cas9 transfected NIH3T3 cells fixed with100% methanol (5 min)</p>" } } }
style
left-column
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CRISPR-Cas9 also known simply as Cas9 is a protein that acts as a molecular scissor in gene editing. It has a molecular weight of approximately 160 kDa. Cas9 is a part of the CRISPR-Cas system originally discovered in bacteria where it serves as an adaptive immune system. In bacterial cells Cas9 targets and cleaves specific DNA sequences allowing for targeted gene modifications. While the expression of the CRISPR-Cas system occurs naturally in prokaryotes scientists now harness it in various organisms for genetic manipulation.
Biological function summary

The Cas9 protein functions as an integral part of the CRISPR-Cas9 complex which includes a guide RNA to direct the protein to specific DNA sequences. This complex enables precise cuts at targeted locations within the genome. The Cas9 protein size allows it to fit effectively within cells facilitating genome editing in areas such as research agriculture and therapeutics. The complexity of CRISPR-Cas9 also includes the interaction with other cellular components that assist in DNA repair post-cleavage.

Pathways

CRISPR-Cas9 plays a role in DNA repair pathways particularly non-homologous end joining and homologous recombination. After Cas9-induced DNA breaks these pathways become active to repair the cleaved DNA. The gene editing process facilitated by CRISPR-Cas9 often involves interaction with DNA repair proteins like ku70/80 and Rad51 in response to induced breaks. This allows for either the incorporation of new genetic material or the modification of existing genes.

CRISPR-Cas9 has potential in treating genetic disorders such as cystic fibrosis and Duchenne muscular dystrophy. By correcting mutated genes scientists aim to restore normal gene function offering potential therapeutic benefits. Additionally CRISPR-Cas9 relates indirectly to p53 a protein known for its tumor suppressor functions because gene-editing processes can sometimes activate p53-dependent pathways leading to cell cycle arrest or apoptosis. This highlights the importance of understanding the broader implications of CRISPR-Cas9 in therapeutic applications.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) (PubMed : 21455174). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA; Cas9 only stabilizes the pre-crRNA : tracrRNA interaction and has no catalytic function in RNA processing (PubMed : 24270795). Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed 3'-5' exonucleolytically. DNA-binding requires protein and both gRNAs, as does nuclease activity. Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. DNA strand separation and heteroduplex formation starts at PAM sites; PAM recognition is required for catalytic activity (PubMed : 24476820). Confers immunity against a plasmid with homology to the appropriate CRISPR spacer sequences (CRISPR interference) (PubMed : 21455174).
See full target information cas9

Additional targets

CRISPR-Cas9
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 16:e0259812 PubMed34752487

2021

Protocol for assessment of the efficiency of CRISPR/Cas RNP delivery to different types of target cells.

Applications

Unspecified application

Species

Unspecified reactive species

Marina A Tyumentseva,Aleksandr I Tyumentsev,Vasiliy G Akimkin
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB202657

Anti-CRISPR-Cas9 antibody [EPR19633]

primary-antibodies

crispr-cas9-antibody-epr19633-ab202657

0

(0 reviews)

Alternative Product
Primary Antibodies

AB203933

Anti-CRISPR-Cas9 antibody [EPR19799]

primary-antibodies

crispr-cas9-antibody-epr19799-ab203933

3

(2 reviews)

Alternative Product
Primary Antibodies

AB202638

Anti-CRISPR-Cas9 antibody [EPR19620]

primary-antibodies

crispr-cas9-antibody-epr19620-ab202638

0

(0 reviews)

Alternative Product
Primary Antibodies

AB210752

Anti-CRISPR-Cas9 antibody [EPR19619-92]

primary-antibodies

crispr-cas9-antibody-epr19619-92-ab210752

0

(0 reviews)

Alternative Product
Primary Antibodies

AB203943

Anti-CRISPR-Cas9 antibody [EPR19795]

primary-antibodies

crispr-cas9-antibody-epr19795-ab203943

0

(0 reviews)

Alternative Version
Primary Antibodies

AB215240

Alexa Fluor® 647 Anti-CRISPR-Cas9 antibody [EPR18991]

primary-antibodies

alexa-fluor-647-crispr-cas9-antibody-epr18991-ab215240

0

(0 reviews)

Alternative Version
Primary Antibodies

AB232379

Anti-CRISPR-Cas9 antibody [EPR18991] - BSA and Azide free

primary-antibodies

crispr-cas9-antibody-epr18991-bsa-and-azide-free-ab232379

0

(0 reviews)

Alternative Version
Primary Antibodies

AB215345

HRP Anti-CRISPR-Cas9 antibody [EPR18991]

primary-antibodies

hrp-crispr-cas9-antibody-epr18991-ab215345

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com