JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB190194

Alexa Fluor® 488 Anti-Cyclin D1 antibody [EPR2241]

Be the first to review this product! Submit a review

|

(7 Publications)

Rabbit Recombinant Monoclonal Cyclin D1 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 7 publications.

View Alternative Names

BCL1, PRAD1, CCND1, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1 oncogene, PRAD1 oncogene, BCL-1

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cyclin D1 antibody [EPR2241] (AB190194)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cyclin D1 antibody [EPR2241] (AB190194)

ab190194 staining Cyclin D1 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab190194 at a working dilution of 1 on 50 (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594, shown in red) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2241

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

The protein expressed by the CCND1 gene acts as a regulatory component of the cyclin D1-CDK4 (DC) complex, which phosphorylates and inhibits retinoblastoma (RB) protein family members, including RB1, regulating the cell cycle during the G(1)/S transition. Phosphorylation of RB1 allows the transcription factor E2F to dissociate from the RB/E2F complex, enabling transcription of E2F target genes responsible for G(1) phase progression. In early G(1) phase, it hypophosphorylates RB1. Cyclin D-CDK4 complexes integrate various mitogenic and antimitogenic signals. The protein also serves as a substrate for SMAD3, phosphorylating it in a cell-cycle-dependent manner to repress its transcriptional activity, and forms part of the cyclin D1/CDK4/CDKN1B complex necessary for nuclear translocation and activity. Additionally, it exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters, independent of the cell cycle. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CCND1
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Genome biology 24:199 PubMed37653425

2023

Single-cell transcriptomics reveals multiple chemoresistant properties in leukemic stem and progenitor cells in pediatric AML.

Applications

Unspecified application

Species

Unspecified reactive species

Yongping Zhang,Shuting Jiang,Fuhong He,Yuanyuan Tian,Haiyang Hu,Li Gao,Lin Zhang,Aili Chen,Yixin Hu,Liyan Fan,Chun Yang,Bi Zhou,Dan Liu,Zihan Zhou,Yanxun Su,Lei Qin,Yi Wang,Hailong He,Jun Lu,Peifang Xiao,Shaoyan Hu,Qian-Fei Wang

Translational cancer research 9:5190-5199 PubMed35117886

2020

hsa-miR-195-5p inhibits cell proliferation of human thyroid carcinoma cells via modulation of p21/cyclin D1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dexin Liu,Ping Li,Xiaodong Wang,Wei Wang

Toxicological sciences : an official journal of the Society of Toxicology 176:162-174 PubMed32159786

2020

Arsenic Stimulates Myoblast Mitochondrial Epidermal Growth Factor Receptor to Impair Myogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Amin Cheikhi,Teresa Anguiano,Jane Lasak,Baoli Qian,Amrita Sahu,Hikaru Mimiya,Charles C Cohen,Peter Wipf,Fabrisia Ambrosio,Aaron Barchowsky

Methods in molecular biology (Clifton, N.J.) 2055:521-562 PubMed31502168

2019

Cyclic Multiplexed-Immunofluorescence (cmIF), a Highly Multiplexed Method for Single-Cell Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Eng,Guillaume Thibault,Shiuh-Wen Luoh,Joe W Gray,Young Hwan Chang,Koei Chin

eLife 7: PubMed29993362

2018

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ren Lin,Benjamin Izar,Shu Wang,Clarence Yapp,Shaolin Mei,Parin M Shah,Sandro Santagata,Peter K Sorger

Current protocols in chemical biology 8:251-264 PubMed27925668

2016

Cyclic Immunofluorescence (CycIF), A Highly Multiplexed Method for Single-cell Imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ren Lin,Mohammad Fallahi-Sichani,Jia-Yun Chen,Peter K Sorger

Nature communications 6:8390 PubMed26399630

2015

Highly multiplexed imaging of single cells using a high-throughput cyclic immunofluorescence method.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Ren Lin,Mohammad Fallahi-Sichani,Peter K Sorger
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB226977

Anti-Cyclin D1 antibody

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody (AB226977)

  • 0
  • 0
Primary Antibodies

AB185241

Anti-Cyclin D1 antibody - C-terminal

Western blot - Anti-Cyclin D1 antibody - C-terminal (AB185241)

  • 0
  • 0
Primary Antibodies

AB134175

Anti-Cyclin D1 antibody [EPR2241] - C-terminal

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Cyclin D1 antibody [EPR2241] - C-terminal (AB134175)

  • 5
  • 15
Primary Antibodies

AB16663

Anti-Cyclin D1 antibody [SP4]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials

Western blot - Anti-Cyclin D1 antibody [SP4] (AB16663)

  • 4
  • 23
Primary Antibodies

AB156448

Anti-Cyclin D1 antibody [EPR2241] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [EPR2241] - BSA and Azide free (AB156448)

  • 4
  • 7
Primary Antibodies

AB239794

Anti-Cyclin D1 antibody [SP4] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [SP4] - BSA and Azide free (AB239794)

  • 5
  • 5
Primary Antibodies

AB62151

Anti-Cyclin D1 (phospho T286) antibody

Lab Essentials

Western blot - Anti-Cyclin D1 (phospho T286) antibody (AB62151)

  • 0
  • 0

Complementary Products

Assay Kits

AB214571

Human Cyclin D1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Cyclin D1 ELISA Kit (AB214571)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB192982

Alexa Fluor® 647 Anti-EGFR antibody [EP38Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-EGFR antibody [EP38Y] (AB192982)

  • 0
  • 0
Primary Antibodies

AB300453

Anti-PIM1 antibody [EPR24447-39]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-PIM1 antibody [EPR24447-39] (AB300453)

  • 0
  • 0
Primary Antibodies

AB198944

Alexa Fluor® 488 Anti-Cdk6 antibody [EPR4515]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cdk6 antibody [EPR4515] (AB198944)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com