AB154476

Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11]

Name: Anti-Cytochrome C Alexa Fluor® 488 antibody [37BA11] (ab154476) Mouse monoclonal IgG2a | Abcam
Brand: Abcam Limited
SKU: AB154476

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(3 Publications)

Mouse Monoclonal Cytochrome C antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications.

View Alternative Names

CYC, CYCS, Cytochrome c

3 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (AB154476)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (AB154476)

Immunocytochemistry with HDFn (100x) cells were stained with Anti-Cytochrome C Alexa-488 antibody (1.0 µg/mL, ab154476) in green, Anti-HSP60 (1/1000, ab46798) as red, and DAPI in blue, as a nuclear stain. Secondary antibody used was goat anti-rabbit dyelight-594 (1/1000, ab96897).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (AB154476)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (AB154476)

Flow Cytometry for Anti-Cytochrome C Alexa-488 antibody (ab154476) stained HeLa cells. Flow cytometry results of unstained cells (black) and 0.2 µg/mL Cytochrome C Alexa-488 (red) stained cells.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (AB154476)

Immunocytochemistry with HeLa cells (100x) were stained with Anti-Cytochrome C Alexa-488 antibody (1.0 µg/mL, ab154476) in green and DAPI in blue, as a nuclear stain.

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytochrome C antibody [37BA11]
HRP

HRP Anti-Cytochrome C antibody [37BA11]
Unconjugated

Anti-Cytochrome C antibody [37BA11]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

37BA11

Isotype

IgG2a

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "0.2 µg/mL", "FlowCytIntra-species-notes": "<a href='/en-us/products/unavailable/mouse-igg2a-kappa-monoclonal-10d7a7b2-isotype-control-alexa-fluorreg-488-ab171464'>ab171464</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Caenorhabditis elegans": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification notes

ab154476 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome C also referred to as "cytochrome c" or "Cyt C" is a small protein with a molecular weight of approximately 12 kDa. It is a component of the electron transport chain playing an essential mechanical role in cellular respiration. Cytochrome C is expressed in the mitochondria of eukaryotic cells where it functions as a heme protein. The mass of this protein allows efficient electron transfer between Complex III (cytochrome bc1 complex) and Complex IV (cytochrome c oxidase) contributing to ATP synthesis.

Biological function summary

Cytochrome c contributes to the process of oxidative phosphorylation within mitochondria. It is not only an important electron carrier but it also plays a significant role in apoptosis. In the event of cellular stress cytochrome c releases into the cytosol and forms part of the apoptosome complex. This apoptosome activation leads to the caspase cascade driving programmed cell death. Therefore cytochrome C serves as both a mediator of energy production and a regulator of apoptosis.

Pathways

Cytochrome c is integral to the mitochondrial electron transport chain and apoptotic pathways. It interacts with proteins in these pathways such as cytochrome c oxidase in oxidative phosphorylation and Apaf-1 during apoptosis. The electron transport chain's function is dependent on the efficient transfer of electrons facilitated by cytochrome c ensuring ATP production in cellular respiration.

Cytochrome c links to conditions like cancer and neurodegenerative diseases. In cancer its role in the apoptotic pathway often emerges as cancer cells can develop mechanisms to evade apoptosis impacting cytochrome c release. For neurodegenerative diseases disruptions in mitochondrial function and cytochrome c's release can facilitate cell death implicating its role in conditions like Parkinson's disease. The interaction of cytochrome c with proteins such as p53 in response to cellular stress further highlights its involvement in disease pathogenesis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

See full target information CYCS

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 14:5205-5210 PubMed27779720

2016

PACE4 regulates apoptosis in human pancreatic cancer Panc‑1 cells via the mitochondrial signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Feng Tian,Guo-Min Huang,Hong-Liang Zang,Hong Cao

Cell death and differentiation 23:2031-2041 PubMed27588704

2016

The oncolytic compound LTX-401 targets the Golgi apparatus.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhou,Allan Sauvat,Lígia C Gomes-da-Silva,Sylvère Durand,Sabrina Forveille,Kristina Iribarren,Takahiro Yamazaki,Sylvie Souquere,Lucillia Bezu,Kevin Müller,Marion Leduc,Peng Liu,Liwei Zhao,Aurélien Marabelle,Laurence Zitvogel,Øystein Rekdal,Oliver Kepp,Guido Kroemer

Oncotarget 6:26599-614 PubMed26378049

2015

The oncolytic peptide LTX-315 kills cancer cells through Bax/Bak-regulated mitochondrial membrane permeabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Heng Zhou,Sabrina Forveille,Allan Sauvat,Valentina Sica,Valentina Izzo,Sylvère Durand,Kevin Müller,Peng Liu,Laurence Zitvogel,Øystein Rekdal,Oliver Kepp,Guido Kroemer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (AB154476)

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (AB154476)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytochrome C antibody [37BA11] (AB154476)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise