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Mouse Monoclonal Cytokeratin 18 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing KRT18 protein.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [C04] (AB187573), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 99% PBS, 0.2% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05783

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested
Mammals
Predicted

Tested
Tested

Species
Human
Dilution info
3.00000-12.00000 µg/mL
Notes

ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mammals
Dilution info
-
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Alternative names

Recommended products

Mouse Monoclonal Cytokeratin 18 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing KRT18 protein.

Key facts

Isotype
IgG1
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing KRT18 protein. The exact immunogen used to generate this antibody is proprietary information. Database link P05783
Clone number
C04
Purification technique
Size-exclusion chromatography
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Store in the dark

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytokeratin 18 (CK18) also known as Keratin 18 or KRT18 is a type I intermediate filament protein with a molecular weight of approximately 45 kDa. It plays a mechanical role in stabilizing epithelial cell structure and function. CK18 is expressed in single-layered epithelial tissues such as the liver intestine and pancreas. It partners with the type II keratin usually KRT8 to form a stable cytoskeletal network in these cells providing structural integrity and resilience.

Biological function summary

CK18 maintains cellular integrity and mediates resistance to mechanical and non-mechanical stresses in epithelial cells. It forms heterodimers with KRT8 participating in a complex network of filaments important for maintaining cell shape and stability. This organization plays a role in managing cell survival during stress and apoptosis helping regulate cellular functions and responses in tissues where it is expressed.

Pathways

Cytokeratin 18 is involved in the apoptosis and cellular stress response pathways. It interacts with proteins such as caspases during apoptosis facilitating cytoskeletal reorganization and cell fragmentation. CK18 in coordination with KRT8 also participates in signaling pathways that regulate cell cycle and apoptotic processes underlining its importance in cellular turnover and tissue homeostasis.

Associated diseases and disorders

Elevated levels of CK18 fragments are often observed in cases of non-alcoholic steatohepatitis (NASH) and breast cancer. It serves as a biomarker for liver injury due to its abundance in hepatocytes and release upon cell death. In breast cancer alterations in CK18 expression can reflect disease progression where it interacts with other proteins such as vimentin indicating epithelial-to-mesenchymal transition a process that facilitates metastasis.

Product promise

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1 product image

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [C04] (ab187573), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 18 antibody [C04] (ab187573)

    Intracellular Flow Cytometry analysis (intracellular staining) of cytokeratin 18 in HeLa cells with ab187573 at 2 ?g/ml

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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