Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EPR1579Y]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EPR1579Y] (AB205445)

Immunohistochemical analysis of formalin fixed paraffin embedded human pancreas labelling Cytokeratin 19 with ab205445 at a dilution of 1/100 (5 µg/mL). Antigen retrieval was heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Image inset shows absence of staining in dilution buffer only control.

Positive staining on human pancreas.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EPR1579Y] (AB205445)

Immunohistochemical analysis of formalin fixed paraffin embedded human gastric cancer labelling Cytokeratin 19 with ab205445 at a dilution of 1/100 (5 µg/mL). Antigen retrieval was heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Image inset shows absence of staining in dilution buffer only control.

Positive staining on human gastric cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EPR1579Y] (AB205445)

Immunohistochemical analysis of formalin fixed paraffin embedded human lung cancer labelling Cytokeratin 19 with ab205445 at a dilution of 1/100 (5 µg/mL). Antigen retrieval was heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Image inset shows absence of staining in dilution buffer only control.

Positive staining on human lung cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EPR1579Y] (AB205445)

Immunohistochemical analysis of formalin fixed paraffin embedded human glioma labelling Cytokeratin 19 with ab205445 at a dilution of 1/100 (5 µg/mL). Antigen retrieval was heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins. Image inset shows absence of staining in dilution buffer only control.

Negative control : no staining on human glioma.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 19 antibody [EPR1579Y] (AB205445)

ab205445 staining Cytokeratin 19 in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205445 at 1/1000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5min).