AB185048

Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

Name: Alexa Fluor® 488 conjugated Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab185048) | Abcam
Brand: Abcam Limited
SKU: AB185048
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(11 Publications)

Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker - Alexa Fluor® 488 conjugated (ab185048) is a rabbit recombinant monoclonal antibody detecting Cytokeratin 7 in Flow Cytometry (Intra), ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

SCL, KRT7, Cytokeratin-7, Keratin-7, Sarcolectin, Type-II keratin Kb7, CK-7, K7, CK7

3 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB185048)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB185048)

ab185048 staining Cytokeratin 7 in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab185048 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor^® 594, shown in red) at 2μg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.

This product gave a positive signal in 100% methanol (5 min) fixed A549 cells under the same testing conditions.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB185048)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB185048)

Overlay histogram showing T47D cells stained with ab185048 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab185048, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This antibody gave a positive signal in T47D cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB185048)

ab185048 staining Cytokeratin 7 in HeLa cells. The cells were fixed with 4% PFA (10min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab185048 at 1/50 dilution overnight at +4°C (shown in green). AlexaFluor^®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410).

Carrier free

Anti-Cytokeratin 7 antibody [EPR1619Y] - BSA and Azide free
Unconjugated

Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker
578 PE

PE Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR1619Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human, Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/alexa-fluor-488-rabbit-igg-monoclonal-epr25a-isotype-control-ab199091'>ab199091</a> - Rabbit monoclonal IgG (Alexa FluorÂ® 488), is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab185048) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPR1619Y is also available pre-conjugated to a variety of labels for your convenience – Anti-Cytokeratin 7 Alexa Fluor® 488 [EPR1619Y] - Cytoskeleton Marker (ab185048).

Other related products
We have a range of other formats of antibody clone [EPR1619Y] also available for your convenience: ab68459, Carrier free - ab181831, Alexa Fluor® 488 - ab185048, Alexa Fluor® 647 - ab192077, HRP - ab192079, Alexa Fluor® 594 - ab203427, Alexa Fluor® 555 - ab203434, PE - ab210628, Carrier free - ab302460

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).

See full target information KRT7

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

BMC biology 20:276 PubMed36482461

2022

An IGF1-expressing endometrial stromal cell population is associated with human decidualization.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Wei Shi,Zhen-Zhen Lai,Hui-Li Yang,Wen-Jie Zhou,Xiao-Ya Zhao,Feng Xie,Song-Ping Liu,Wei-Dong Chen,Tao Zhang,Jiang-Feng Ye,Xiang-Yu Zhou,Ming-Qing Li

Theranostics 12:6527-6547 PubMed36185612

2022

Single-cell transcriptome profiling of the human endometrium of patients with recurrent implantation failure.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen-Zhen Lai,Yun Wang,Wen-Jie Zhou,Zhou Liang,Jia-Wei Shi,Hui-Li Yang,Feng Xie,Wei-Dong Chen,Rui Zhu,Ce Zhang,Jie Mei,Jian-Yuan Zhao,Jiang-Feng Ye,Tao Zhang,Ming-Qing Li

Cell reports methods 1: PubMed34485971

2021

Toward reproducible, scalable, and robust data analysis across multiplex tissue imaging platforms.

Applications

Unspecified application

Species

Unspecified reactive species

Erik A Burlingame,Jennifer Eng,Guillaume Thibault,Koei Chin,Joe W Gray,Young Hwan Chang

Environmental health perspectives 129:27005 PubMed33605785

2021

Bisphenol S and Epidermal Growth Factor Receptor Signaling in Human Placental Cytotrophoblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Elvis Ticiani,Jeremy Gingrich,Yong Pu,Mathew Vettathu,Jacquelyn Davis,Denny Martin,Margaret G Petroff,Almudena Veiga-Lopez

Clinical therapeutics : PubMed33541739

2021

Differential Expression of Rab5 and Rab7 Small GTPase Proteins in Placental Tissues From Pregnancies Affected by Maternal Coronavirus Disease 2019.

Applications

Unspecified application

Species

Unspecified reactive species

Yoel Benarroch,Lillian Juttukonda,Vishakha Sabharwal,Jeffery Boateng,Amir R Khan,Christina Yarrington,Elisha M Wachman,Elizabeth Taglauer

Cell 178:160-175.e27 PubMed31155233

2019

Stromal Microenvironment Shapes the Intratumoral Architecture of Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Matteo Ligorio,Srinjoy Sil,Jose Malagon-Lopez,Linda T Nieman,Sandra Misale,Mauro Di Pilato,Richard Y Ebright,Murat N Karabacak,Anupriya S Kulkarni,Ann Liu,Nicole Vincent Jordan,Joseph W Franses,Julia Philipp,Johannes Kreuzer,Niyati Desai,Kshitij S Arora,Mihir Rajurkar,Elad Horwitz,Azfar Neyaz,Eric Tai,Neelima K C Magnus,Kevin D Vo,Chittampalli N Yashaswini,Francesco Marangoni,Myriam Boukhali,Jackson P Fatherree,Leah J Damon,Kristina Xega,Rushil Desai,Melissa Choz,Francesca Bersani,Adam Langenbucher,Vishal Thapar,Robert Morris,Ulrich F Wellner,Oliver Schilling,Michael S Lawrence,Andrew S Liss,Miguel N Rivera,Vikram Deshpande,Cyril H Benes,Shyamala Maheswaran,Daniel A Haber,Carlos Fernandez-Del-Castillo,Cristina R Ferrone,Wilhelm Haas,Martin J Aryee,David T Ting

Development (Cambridge, England) 140:3965-76 PubMed24004950

2013

BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a ΔNp63+ cytotrophoblast stem cell state.

Applications

Unspecified application

Species

Unspecified reactive species

Yingchun Li,Matteo Moretto-Zita,Francesca Soncin,Anna Wakeland,Lynlee Wolfe,Sandra Leon-Garcia,Raj Pandian,Donald Pizzo,Li Cui,Kristopher Nazor,Jeanne F Loring,Christopher P Crum,Louise C Laurent,Mana M Parast

Journal of virology 86:12795-805 PubMed22973041

2012

Impaired surfactant production by alveolar epithelial cells in a SCID-hu lung mouse model of congenital human cytomegalovirus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ekaterina Maidji,Galina Kosikova,Pheroze Joshi,Cheryl A Stoddart

Proceedings of the National Academy of Sciences of 108:7547-52 PubMed21502498

2011

Modeling high-grade serous ovarian carcinogenesis from the fallopian tube.

Applications

Unspecified application

Species

Unspecified reactive species

Alison M Karst,Keren Levanon,Ronny Drapkin

Cancer research 71:3246-56 PubMed21430067

2011

mTORC1 and mTORC2 regulate EMT, motility, and metastasis of colorectal cancer via RhoA and Rac1 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Pat Gulhati,Kanika A Bowen,Jianyu Liu,Payton D Stevens,Piotr G Rychahou,Min Chen,Eun Y Lee,Heidi L Weiss,Kathleen L O'Connor,Tianyan Gao,B Mark Evers

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB185048)

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB185048)

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (AB185048)

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Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

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Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (11)

Product promise