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AB326532

Alexa Fluor® 488 Anti-DC-SIGN antibody [UW60.1]

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Mouse Monoclonal DC-SIGN antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD209, CLEC4L, CD209 antigen, C-type lectin domain family 4 member L, Dendritic cell-specific ICAM-3-grabbing non-integrin 1, DC-SIGN, DC-SIGN1

3 Images
Flow Cytometry - Alexa Fluor® 488 Anti-DC-SIGN antibody [UW60.1] (AB326532)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-DC-SIGN antibody [UW60.1] (AB326532)

Flow cytometry surface staining pattern of human GM-CSF and IL-4 stimulated peripheral blood mononuclear cells using ab326532 (concentration in sample 5 μg/ml).

Flow Cytometry - Alexa Fluor® 488 Anti-DC-SIGN antibody [UW60.1] (AB326532)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-DC-SIGN antibody [UW60.1] (AB326532)

Separation of human CD209 positive CD22c positive cells (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human GM-CSF and IL-4 stimulated peripheral blood mononuclear cells stained using ab326532 (concentration in sample 5 μg/ml).

Flow Cytometry - Alexa Fluor® 488 Anti-DC-SIGN antibody [UW60.1] (AB326532)
  • Flow Cyt

Supplier Data

Flow Cytometry - Alexa Fluor® 488 Anti-DC-SIGN antibody [UW60.1] (AB326532)

Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD11c (BU15) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and ab326532 (concentration in sample 5 μg/ml).

  • 660 APC

    APC Anti-DC-SIGN antibody [UW60.1]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-DC-SIGN antibody [UW60.1]

  • 578 PE

    PE Anti-DC-SIGN antibody [UW60.1]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD209 antibody [UW60.1]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD209 antibody [UW60.1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UW60.1

Isotype

IgG2a

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Specificity

The mouse monoclonal antibody UW60.1 recognizes an extracellular epitope of human CD209 (DC-SIGN), a 44 kDa transmembrane receptor, expressed on the surface of dendritic cells and macrophages.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Size-exclusion chromatography
Purification notes
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Storage buffer
pH: 7.4 Preservative: 0.098% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze|Store in the dark
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response.. On dendritic cells (DCs) it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates (PubMed : 10721994, PubMed : 10721995, PubMed : 11017109, PubMed : 12574325). May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2 (PubMed : 11017109). Forms a first contact between DC and resting T cell, througth ICAM3 binding, facilitating the downstream DC-T cell clustering process and DC-induced proliferation of resting T Cells (PubMed : 10721994, PubMed : 10721995).. (Microbial infection) Acts as an attachment receptor for HIV-1 and HIV-2.. (Microbial infection) Acts as an attachment receptor for Ebolavirus.. (Microbial infection) Acts as an attachment receptor for Cytomegalovirus.. (Microbial infection) Acts as an attachment receptor for HCV.. (Microbial infection) Acts as an attachment receptor for Dengue virus.. (Microbial infection) Acts as an attachment receptor for Measles virus.. (Microbial infection) Acts as an attachment receptor for Herpes simplex virus 1.. (Microbial infection) Acts as an attachment receptor for Influenzavirus A.. (Microbial infection) Acts as an attachment receptor for SARS-CoV.. (Microbial infection) Acts as an attachment receptor for Japanese encephalitis virus.. (Microbial infection) Acts as an attachment receptor for Lassa virus (PubMed : 23966408). Acts as an attachment receptor for Marburg virusn.. (Microbial infection) Acts as an attachment receptor for Respiratory syncytial virus.. (Microbial infection) Acts as an attachment receptor for Rift valley fever virus and uukuniemi virus.. (Microbial infection) Acts as an attachment receptor for West-nile virus.. (Microbial infection) Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of bacterial pathogen antigens, including Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA (PubMed : 16379498). Recognition of M.tuberculosis by dendritic cells occurs partially via this molecule (PubMed : 16092920, PubMed : 21203928).
See full target information CD209
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