Alexa Fluor® 488 Anti-DCAMKL1 antibody [EPR6085]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-DCAMKL1 antibody [EPR6085] (AB202754)

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling DCAMKL1 with ab202754 at 1/100 dilution.

Negative control : no staining on rat skeletal muscle.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-DCAMKL1 antibody [EPR6085] (AB202754)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling DCAMKL1 with ab202754 at 1/100 dilution.

Sporadic staining on mouse colon.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-DCAMKL1 antibody [EPR6085] (AB202754)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling DCAMKL1 with ab202754 at 1/100 dilution.

Sporadic staining on mouse stomach.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-DCAMKL1 antibody [EPR6085] (AB202754)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling DCAMKL1 with ab202754 at 1/100 dilution.

Sporadic staining on rat colon.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-DCAMKL1 antibody [EPR6085] (AB202754)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling DCAMKL1 with ab202754 at 1/100 dilution.

Negative control : no staining on mouse skeletal muscle.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-DCAMKL1 antibody [EPR6085] (AB202754)

Immunohistochemical analysis of paraffin-embedded Rat stomach tissue labeling DCAMKL1 with ab202754 at 1/100 dilution.

Sporadic staining on rat stomach.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-DCAMKL1 antibody [EPR6085] (AB202754)

ab202754 staining DCAMKL1 in Neuro2a cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202754 at 1/5000 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).