AB245892

Alexa Fluor® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal DDDDK tag (Binds to FLAG® tag sequence) antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Tag samples. Cited in 5 publications.

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB245892)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB245892)

ab245892 staining DDDDK tag in 293T cells transfected with a FLAG^®-tagged PFKFB3 expression vector. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab245892 at 1/50 dilution (shown in green) and a Mouse monoclonal anti-FLAG^® M2 antibody used as a counterstain. This was followed by an incubation at room temperature for 1h with ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^®594), at 1/250 dilution (shown in red). Nuclear DNA was labeled with DAPI (shown in blue).

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]
HRP

HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]
Unconjugated

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]
Carrier free

Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20018-251

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p>This product gave a positive signal in FLAG®-tagged PFKFB3 expression construct transfected 293T cells fixed with 4% formaldehyde (10 min).</p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

FLAG® is a registered trade mark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information DDDDK tag (Binds to FLAG® tag sequence)

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1468258 PubMed39539545

2024

CRISPR/Cas9-mediated knockout of STAT1 in porcine-derived cell lines to elucidate the role of STAT1 in autophagy following classical swine fever virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Liyuan Zhang,Dongli Liang,Kaijun Min,Jiaxin Liang,Yu Tian,Cheng Liu,Ting Rong Luo,Xiaoning Li

Journal of nanobiotechnology 22:563 PubMed39272146

2024

Circ_0001947 encapsulated by small extracellular vesicles promotes gastric cancer progression and anti-PD-1 resistance by modulating CD8 T cell exhaustion.

Applications

Unspecified application

Species

Unspecified reactive species

Bingyu Wang,Wenbo Liu,Mingming Zhang,Yong Li,Hongyue Tang,Yingying Wang,Chao Song,Buyun Song,Bibo Tan

Methods in molecular biology (Clifton, N.J.) 2593:113-126 PubMed36513927

2022

Labeling of Phospho-Specific Antibodies with oYo-Link® Epitope Tags for Multiplex Immunostaining.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwen Niu,Jodi Hagen,Feifan Yu,Alexander E Kalyuzhny,Andrew Tsourkas

Molecular therapy. Nucleic acids 30:300-310 PubMed36320322

2022

Cytidine-containing tails robustly enhance and prolong protein production of synthetic mRNA in cell and .

Applications

Unspecified application

Species

Unspecified reactive species

Cheuk Yin Li,Zhenghua Liang,Yaxin Hu,Hongxia Zhang,Kharis Daniel Setiasabda,Jiawei Li,Shaohua Ma,Xiaojun Xia,Yi Kuang

PLoS pathogens 18:e1010506 PubMed35533206

2022

A nonstructural protein encoded by a rice reovirus induces an incomplete autophagy to promote viral spread in insect vectors.

Applications

Unspecified application

Species

Unspecified reactive species

Dongsheng Jia,Qifu Liang,Huan Liu,Guangjun Li,Xiaofeng Zhang,Qian Chen,Aiming Wang,Taiyun Wei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] (AB245892)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (5)

Product promise