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Rabbit Recombinant Monoclonal DESP antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Desmoplakin antibody [EPR4383(2)] (AB208385), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Major high molecular weight protein of desmosomes. Regulates profibrotic gene expression in cardiomyocytes via activation of the MAPK14/p38 MAPK signaling cascade and increase in TGFB1 protein abundance (By similarity).

Alternative names

Desmoplakin, DP, 250/210 kDa paraneoplastic pemphigus antigen, DSP

Recommended products

Rabbit Recombinant Monoclonal DESP antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4383(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Desmoplakin is a protein that plays an integral role in linking intermediate filaments to desmosomal plaques. This protein is also known by its alternate name "DSP" and has a molecular mass of approximately 332 kDa. Desmoplakin is expressed in tissues where mechanical strength is necessary such as the heart and skin. Its expression ensures structural integrity and resilience in areas that experience substantial mechanical stress.

Biological function summary

Desmoplakin acts to maintain cell-cell adhesion through its involvement with desmosomes and is an essential component of the desmosomal complex. This complex is important for the architectural stability of epithelial and cardiac tissues. Desmoplakin interacts directly with other desmosomal proteins like plakoglobin and plakophilin forming a bridge between the cell membrane and the cytoskeleton.

Pathways

Desmoplakin is an important player in the maintenance and function of the cytoskeletal network particularly within the intermediate filament organization. In addition desmoplakin participates in cellular adhesion signaling pathways. Its interaction with proteins such as plakoglobin mediates the organization of desmosomes influencing cellular signaling and communication processes critical for tissue morphogenesis and repair.

Associated diseases and disorders

Mutations or malfunctions of desmoplakin are linked to conditions like arrhythmogenic right ventricular cardiomyopathy (ARVC) and skin disorders such as striate palmoplantar keratoderma. In these diseases desmoplakin's ability to maintain structural integrity is compromised leading to the disruption of tissue architecture. In ARVC for example the faulty interaction between desmoplakin and plakoglobin may cause abnormal heart muscle function contributing to the pathology of the disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Desmoplakin antibody [EPR4383(2)] (ab208385), expandable thumbnail

    Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Desmoplakin antibody [EPR4383(2)] (ab208385)

    Overlay histogram showing A431 cells stained with ab208385 (red line). The cells were fixed with 80% methanol (10 min) and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208385, 1/50 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 (Alexa Fluor® 488 Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com