AB225519

Alexa Fluor® 488 Anti-DFNA5/GSDME antibody [EPR19859]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal DFNA5/GSDME antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DFNA5, ICERE1, GSDME, Gasdermin-E, Inversely correlated with estrogen receptor expression 1, Non-syndromic hearing impairment protein 5, ICERE-1

1 Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-DFNA5/GSDME antibody [EPR19859] (AB225519)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-DFNA5/GSDME antibody [EPR19859] (AB225519)

Overlay histogram showing SHSY5Y cells stained with ab225519 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab225519, 1/50 dilution) for 30 min at 22°C.

Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor^® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

578 PE

PE Anti-DFNA5/GSDME antibody [EPR19859]
Unconjugated

Anti-DFNA5/GSDME antibody [EPR19859] - N-terminal
Carrier free

Anti-DFNA5/GSDME antibody [EPR19859] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19859

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p>The cellular localisation of this product has been verified in ICC/IF.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DFNA5 also known as GSDME (Gasdermin E) is a protein encoded by the GSDME gene. It has a molecular mass of approximately 59 kDa. The protein is expressed in various tissues including the cochlea in the inner ear and some epithelial tissues. Mechanically DFNA5/GSDME plays a role in inducing cell membrane pore formation leading to cell lysis. This activity relates to its involvement in processes like cell death specifically pyroptosis where the cell undergoes a form of programmed necrosis.

Biological function summary

DFNA5/GSDME serves as a pore-forming protein that facilitates pyroptotic cell death usually upon cleavage by caspase-3. This activity is important for innate immune response and the maintenance of cellular homeostasis. DFNA5/GSDME operates independently and does not form part of large protein complexes. The cleaved form inserts into the lipid bilayer of cell membranes contributing to the execution of cell death particularly under stress conditions or cellular insult.

Pathways

DFNA5/GSDME is involved in the pyroptosis and apoptosis pathways. It plays an essential role in the cellular response to inflammation and stress signals by interacting with caspase-3 a critical protease in the apoptosis pathway. The balance between apoptosis and pyroptosis decides cell fate with DFNA5/GSDME activation tipping towards pyroptotic cell death. The interconnection with other gasdermin family proteins such as GSDMD is notable as they share functional and structural similarities in promoting necrosis-like cell death processes.

Mutations in the DFNA5 gene relate to progressive hearing loss (nonsyndromic sensorineural deafness). Dysregulation of GSDME has also been implicated in cancer where altered expression promotes tumorigenesis or tumor suppression depending on the context. In hearing loss GSDME's interaction with pathways influencing apoptosis highlights its pathogenic role while in cancer connections to caspase-3 show a potential dichotomy in cell survival and cell death regulation.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Gasdermin-E. Precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis (PubMed : 27281216, PubMed : 28459430, PubMed : 33852854, PubMed : 35594856, PubMed : 36607699). This form constitutes the precursor of the pore-forming protein : upon cleavage, the released N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores, triggering pyroptosis (PubMed : 28459430).. Gasdermin-E, N-terminal. Pore-forming protein produced by cleavage by CASP3 or granzyme B (GZMB), which converts non-inflammatory apoptosis to pyroptosis or promotes granzyme-mediated pyroptosis, respectively (PubMed : 27281216, PubMed : 28459430, PubMed : 32188940, PubMed : 33852854, PubMed : 35594856). After cleavage, moves to the plasma membrane, homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, allowing the release of mature interleukins (IL1B and IL16) and triggering pyroptosis (PubMed : 28459430, PubMed : 32188940, PubMed : 33852854, PubMed : 35594856). Binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate (PubMed : 28459430). Cleavage by CASP3 switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis (PubMed : 27281216, PubMed : 28459430, PubMed : 32188940). Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents (PubMed : 28045099). Exhibits bactericidal activity (PubMed : 27281216). Cleavage by GZMB promotes tumor suppressor activity by triggering robust anti-tumor immunity (PubMed : 21522185, PubMed : 32188940). Suppresses tumors by mediating granzyme-mediated pyroptosis in target cells of natural killer (NK) cells : cleavage by granzyme B (GZMB), delivered to target cells from NK-cells, triggers pyroptosis of tumor cells and tumor suppression (PubMed : 31953257, PubMed : 32188940). May play a role in the p53/TP53-regulated cellular response to DNA damage (PubMed : 16897187).. Gasdermin-E, N-terminal. (Microbial infection) Pore-forming protein, which promotes maternal placental pyroptosis in response to Zika virus infection, contributing to adverse fetal outcomes.

See full target information GSDME

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC medical genomics 16:133 PubMed37322474

2023

Single-cell RNA-sequencing of stria vascularis cells in the adult Slc26a4 mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Young Koh,Corentin Affortit,Paul T Ranum,Cody West,William D Walls,Hidekane Yoshimura,Jian Q Shao,Brian Mostaert,Richard J H Smith

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-DFNA5/GSDME antibody [EPR19859]

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-DFNA5/GSDME antibody [EPR19859] (AB225519)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise