Alexa Fluor® 488 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB185013)

Overlay histogram showing A431 cells stained with ab185013 (red line). The cells were fixed with 80% methanol (5 min). The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab185013, 1/500 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor^® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This antibody gave a positive signal in A431 fixed with 4% formaldehyde (10 min) used under the same conditions.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB185013)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling E Cadherin with ab185013 at 1/50 dilution.

Positive staining on human colon cancer.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB185013)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling E Cadherin with ab185013 at 1/50 dilution.

Negative control : no staining on human cerebrum.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB185013)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling E Cadherin with ab185013 at 1/50 dilution.

Positive staining on human colon.

The primary antibody was incubated for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.