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AB282169

Alexa Fluor® 488 Anti-EGR2 antibody [EPR23228-40]

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Rabbit Recombinant Monoclonal EGR2 antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Mouse samples.

View Alternative Names

Egr-2, Krox-20, Zfp-25, Egr2, E3 SUMO-protein ligase EGR2, E3 SUMO-protein transferase ERG2, Early growth response protein 2, Zinc finger protein Krox-20, EGR-2

1 Images
Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-EGR2 antibody [EPR23228-40] (AB282169)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-EGR2 antibody [EPR23228-40] (AB282169)

Flow cytometry staining of 2% paraformaldehyde fixed, True-Nuclear buffer© permeabilized C57 BL/6 mouse splenocytes (top) or C57 BL/6 mouse splenocytes treated with ConA (2.5 μg/ml, 24h) (bottom), with ab282169 (right) or Rabbit IgG (monoclonal) Alexa Fluor 488 ® (ab199091) isotype (left). C57 BL/6 mouse splenocytes were incubated for 30 min in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab282169) or Rabbit IgG (monoclonal) Alexa Fluor 488 ® (ab199091) isotype (1x 106 in 100μl at 0.2 μg/ml (1/2500)) for 30 min on ice. The cells were simultaneously stained with CD4.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live lymphocytes.

  • Unconjugated

    Anti-EGR2 antibody [EPR23228-40]

  • 578 PE

    PE Anti-EGR2 antibody [EPR23228-40]

  • Carrier free

    Anti-EGR2 antibody [EPR23228-40] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23228-40

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2500", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EGR2 also known as early growth response 2 or KROX20 refers to a transcription factor with a mass of approximately 53 kDa. This protein plays a significant role in gene expression and regulates various genes involved in cellular growth and differentiation. EGR2 is expressed in various tissues with notable presence in the nervous system particularly within Schwann cells and the developing vertebrate nervous system. It can also be found in immune system cells including T and B cells.
Biological function summary

EGR2 influences the development and function of the peripheral nervous system contributing to processes like nerve myelination. It acts as a transcriptional regulator controlling the expression of genes that are essential for the maintenance of myelin sheaths around nerves. While EGR2 does not directly form part of a protein complex its role as a transcription factor allows it to interact with many other proteins to exert its regulatory functions.

Pathways

EGR2 participates in cellular pathways such as the MAPK/ERK signaling pathway and the immune response pathway. In the MAPK/ERK pathway EGR2 interacts with proteins like ERK1/2 to regulate cell growth and differentiation. In immune response pathways EGR2 modulates the differentiation of T cells impacting immune homeostasis and function by influencing gene expression pertinent to immune regulation.

Mutations or dysregulation of EGR2 can relate to Charcot-Marie-Tooth disease type 1 a peripheral nerve disorder. This neuropathy impacts myelin production where alterations in EGR2 play a role alongside proteins like PMP22 which are also affected in the disorder. Additionally EGR2 expression irregularities have been studied in connection with systemic lupus erythematosus in which immune cell regulation becomes impaired affecting proteins involved in immune signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Sequence-specific DNA-binding transcription factor (PubMed : 11823429, PubMed : 1674431, PubMed : 1969796, PubMed : 31852952). Plays a role in hindbrain segmentation by regulating the expression of a subset of homeobox containing genes and in Schwann cell myelination by regulating the expression of genes involved in the formation and maintenance of myelin (PubMed : 11823429, PubMed : 1674431, PubMed : 1969796, PubMed : 31852952, PubMed : 8093858). Binds to two EGR2-consensus sites EGR2A (5'-CTGTAGGAG-3') and EGR2B (5'-ATGTAGGTG-3') in the HOXB3 enhancer and promotes HOXB3 transcriptional activation (PubMed : 11823429). Binds to specific DNA sites located in the promoter region of HOXA4, HOXB2 and ERBB2 (PubMed : 17938205, PubMed : 1969796, PubMed : 8093858). Regulates hindbrain segmentation by controlling the expression of Hox genes, such as HOXA4, HOXB3 and HOXB2, and thereby specifying odd and even rhombomeres (PubMed : 11823429, PubMed : 1674431). Promotes the expression of HOXB3 in the rhombomere r5 and of HOXB3 in r3 and r5 in the hindbrain (PubMed : 11823429, PubMed : 8093858). Regulates myelination in the peripheral nervous system after birth, possibly by regulating the expression of myelin proteins, such as MPZ, and by promoting the differentiation of Schwann cells (PubMed : 10068633, PubMed : 7935840). Involved in the development of the jaw openener musculature, probably by playing a role in its innervation through trigeminal motor neurons (PubMed : 11509834). May play a role in adipogenesis, possibly by regulating the expression of CEBPB (PubMed : 16054051).. E3 SUMO-protein ligase helping SUMO1 conjugation to its coregulators NAB1 and NAB2, whose sumoylation down-regulates EGR2 transcriptional activity.
See full target information Egr2
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