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AB197727

Alexa Fluor® 488 Anti-Endothelial Cell antibody [RECA-1]

1

(1 Review)

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(3 Publications)

Mouse Monoclonal Endothelial Cell antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-Fr and reacts with Rat samples. Cited in 3 publications.
1 Images
Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Endothelial Cell antibody [RECA-1] (AB197727)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-Endothelial Cell antibody [RECA-1] (AB197727)

IHC image of Endothelial Cell staining in a section of frozen normal rat brain.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature. The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab197727 at 1/100 (shown in green) and counterstained using ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Unconjugated

    Anti-Endothelial Cell antibody [RECA-1]

  • Carrier free

    Anti-Endothelial Cell antibody [RECA-1] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

RECA-1

Isotype

IgG1

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Rat

Applications

IHC-Fr

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>This product gave a positive signal in rat frozen brain tissue fixed with 10% formaldehyde (10 min).</p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Endothelial cells sometimes referred to as endothelium form the thin layer of approximately 1-2 µm thickness that lines the interior surface of blood vessels and lymphatic vessels. They possess a considerable array of roles mainly being a barrier between the vessel lumen and surrounding tissue and they regulate the passage of materials and the transit of white blood cells into and out of the bloodstream. Endothelial cells are widespread found in veins arteries capillaries and the heart. These cells express a variety of markers used to identify them and antibodies targeting endothelial markers are valuable tools in research and diagnostics.
Biological function summary

Endothelial cells maintain vascular tone and blood fluidity and they mediate angiogenesis which is the development of new blood vessels from pre-existing ones. They achieve these functions through the expression of adhesion molecules and growth factors. Endothelial cells contribute to the formation of a selective permeability barrier and respond to mechanical and chemical signals. They do not function alone; they are part of a larger vascular complex that includes smooth muscle cells and pericytes.

Pathways

Endothelial cells play key roles in the nitric oxide (NO) signaling and angiogenesis pathways. They produce nitric oxide a critical molecule for vasodilation and blood pressure regulation through the action of enzymes like endothelial nitric oxide synthase (eNOS). In the angiogenesis pathway endothelial cells interact with proteins like vascular endothelial growth factor (VEGF) which signals the proliferation and migration processes necessary for new blood vessel formation.

Endothelial dysfunction links to cardiovascular conditions like atherosclerosis and hypertension. Damage or alteration in endothelial cell function can lead to abnormal vascular behavior. Oxidative stress and inflammation often disrupt endothelial activity involving proteins such as E-selectin and intercellular adhesion molecule-1 (ICAM-1) contributing to disease progression. Understanding these associations highlights the importance of endothelial cell studies in addressing cardiovascular disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Endothelial Cell
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 21:e00301 PubMed38241160

2024

Nicotinamide riboside, pterostilbene and ibudilast protect motor neurons and extend survival in ALS mice.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael López-Blanch,Rosario Salvador-Palmer,María Oriol-Caballo,Paz Moreno-Murciano,Ryan W Dellinger,José M Estrela,Elena Obrador

Nature immunology 24:991-1006 PubMed37095377

2023

Dynamic mitochondrial transcription and translation in B cells control germinal center entry and lymphomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yavuz F Yazicioglu,Eros Marin,Ciaran Sandhu,Silvia Galiani,Iwan G A Raza,Mohammad Ali,Barbara Kronsteiner,Ewoud B Compeer,Moustafa Attar,Susanna J Dunachie,Michael L Dustin,Alexander J Clarke

Frontiers in pharmacology 13:850859 PubMed35370657

2022

Liquorice Extract and 18β-Glycyrrhetinic Acid Protect Against Experimental Pyrrolizidine Alkaloid-Induced Hepatotoxicity in Rats Through Inhibiting Cytochrome P450-Mediated Metabolic Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangting Wang,Jiang Ma,Sheng Yao,Yisheng He,Kai-Kei Miu,Qingsu Xia,Peter P Fu,Yang Ye,Ge Lin
View all publications
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