Rabbit Recombinant Monoclonal ENT2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.
IgG
Rabbit
Alexa Fluor® 488
Ex: 495nm, Em: 519nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
ICC/IF | |
---|---|
Human | Tested |
Mouse | Predicted |
Rat | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
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Bidirectional uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis (PubMed:10722669, PubMed:12527552, PubMed:12590919, PubMed:16214850, PubMed:21795683, PubMed:9396714, PubMed:9478986). Functions as a Na(+)-independent, passive transporter (PubMed:9478986). Involved in the transport of nucleosides such as inosine, adenosine, uridine, thymidine, cytidine and guanosine (PubMed:10722669, PubMed:12527552, PubMed:12590919, PubMed:16214850, PubMed:21795683, PubMed:9396714, PubMed:9478986). Also able to transport purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil) (PubMed:16214850, PubMed:21795683). Involved in nucleoside transport at basolateral membrane of kidney cells, allowing liver absorption of nucleoside metabolites (PubMed:12527552). Mediates apical nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis-barrier (PubMed:23639800). Mediates both the influx and efflux of hypoxanthine in skeletal muscle microvascular endothelial cells to control the amount of intracellular hypoxanthine available for xanthine oxidase-mediated ROS production (By similarity).Isoform 3Non functional nucleoside transporter protein for adenosine or thymidine transport. Does not express on cell membrane.
DER12, ENT2, HNP36, ENT2, DER12, SLC29A2, HNP36, Equilibrative nucleoside transporter 2, hENT2, 36 kDa nucleolar protein HNP36, Delayed-early response protein 12, Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter, Solute carrier family 29 member 2, Equilibrative NBMPR-insensitive nucleoside transporter
Rabbit Recombinant Monoclonal ENT2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.
IgG
Rabbit
Alexa Fluor® 488
Ex: 495nm, Em: 519nm
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Monoclonal
EPR11674
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Store in the dark
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
This supplementary information is collated from multiple sources and compiled automatically.
ENT2 also known as Equilibrative Nucleoside Transporter 2 facilitates the transport of nucleosides across cellular membranes. It weighs approximately 56 kDa. The transporter is widely expressed in various tissues including the brain heart and placenta. By allowing the movement of nucleosides ENT2 plays a fundamental role in nucleoside salvage an important process for maintaining cellular nucleotide pools especially in energy-demanding tissues.
ENT2 participates in the regulation of intracellular nucleoside levels which impacts DNA and RNA synthesis. It operates individually and does not form part of a larger protein complex allowing it to specifically affect the transport rates based on cellular requirements. This role supports essential cellular processes like cell growth and division highlighting ENT2 as an important regulator in genomic stability and cellular homeostasis.
Four words not connected to nucleoside metabolism are vital choices. ENT2 plays major roles in the purine and pyrimidine salvage pathways. These pathways involve essential enzymes that facilitate the recycling of nucleosides for nucleotide synthesis. Related proteins include ENT1 another member of the equilibrative nucleoside transporter family which shares functional similarities in nucleoside transport across membranes and influences the dynamics of these pathways.
Four words unrelated to neurological conditions deserve attention. Dysfunction of ENT2 links to conditions like myocardial ischemia and neurodegenerative disorders. In these conditions ENT2's role in regulating nucleoside availability directly impacts cellular stress responses. For instance its interaction with nucleoside transport can modulate the extracellular adenosine levels influencing the purinergic signaling pathways. In cardiac tissues altered ENT2 function affects the availability of nucleosides during ischemic events potentially exacerbating tissue damage while in neurodegenerative contexts its regulation of nucleoside levels can impact neuron survival and function potentially involving proteins like adenosine deaminase.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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ab201255 staining ENT2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201255 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).
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