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Rabbit Recombinant Monoclonal ENT2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ENT2 antibody [EPR11674] (AB201255), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Bidirectional uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis (PubMed:10722669, PubMed:12527552, PubMed:12590919, PubMed:16214850, PubMed:21795683, PubMed:9396714, PubMed:9478986). Functions as a Na(+)-independent, passive transporter (PubMed:9478986). Involved in the transport of nucleosides such as inosine, adenosine, uridine, thymidine, cytidine and guanosine (PubMed:10722669, PubMed:12527552, PubMed:12590919, PubMed:16214850, PubMed:21795683, PubMed:9396714, PubMed:9478986). Also able to transport purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil) (PubMed:16214850, PubMed:21795683). Involved in nucleoside transport at basolateral membrane of kidney cells, allowing liver absorption of nucleoside metabolites (PubMed:12527552). Mediates apical nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis-barrier (PubMed:23639800). Mediates both the influx and efflux of hypoxanthine in skeletal muscle microvascular endothelial cells to control the amount of intracellular hypoxanthine available for xanthine oxidase-mediated ROS production (By similarity).Isoform 3Non functional nucleoside transporter protein for adenosine or thymidine transport. Does not express on cell membrane.

Alternative names

Recommended products

Rabbit Recombinant Monoclonal ENT2 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples.

Key facts

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR11674

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ENT2 also known as Equilibrative Nucleoside Transporter 2 facilitates the transport of nucleosides across cellular membranes. It weighs approximately 56 kDa. The transporter is widely expressed in various tissues including the brain heart and placenta. By allowing the movement of nucleosides ENT2 plays a fundamental role in nucleoside salvage an important process for maintaining cellular nucleotide pools especially in energy-demanding tissues.

Biological function summary

ENT2 participates in the regulation of intracellular nucleoside levels which impacts DNA and RNA synthesis. It operates individually and does not form part of a larger protein complex allowing it to specifically affect the transport rates based on cellular requirements. This role supports essential cellular processes like cell growth and division highlighting ENT2 as an important regulator in genomic stability and cellular homeostasis.

Pathways

Four words not connected to nucleoside metabolism are vital choices. ENT2 plays major roles in the purine and pyrimidine salvage pathways. These pathways involve essential enzymes that facilitate the recycling of nucleosides for nucleotide synthesis. Related proteins include ENT1 another member of the equilibrative nucleoside transporter family which shares functional similarities in nucleoside transport across membranes and influences the dynamics of these pathways.

Associated diseases and disorders

Four words unrelated to neurological conditions deserve attention. Dysfunction of ENT2 links to conditions like myocardial ischemia and neurodegenerative disorders. In these conditions ENT2's role in regulating nucleoside availability directly impacts cellular stress responses. For instance its interaction with nucleoside transport can modulate the extracellular adenosine levels influencing the purinergic signaling pathways. In cardiac tissues altered ENT2 function affects the availability of nucleosides during ischemic events potentially exacerbating tissue damage while in neurodegenerative contexts its regulation of nucleoside levels can impact neuron survival and function potentially involving proteins like adenosine deaminase.

Product promise

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ENT2 antibody [EPR11674] (ab201255), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ENT2 antibody [EPR11674] (ab201255)

    ab201255 staining ENT2 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab201255 at 1/100 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 100% methanol (5 min).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com