Alexa Fluor® 488 Anti-EpCAM antibody [EPR20532-222]

6 product images

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  Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20532-222] (ab313667)

  Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labelling EpCAM with ab313667 at 1/50 (10.0 ug/ml) dilution. Confocal image showing membranous staining in HCT 116 cells. Negative control: HeLa (PMID:21107020). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20532-222] (ab313667)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling EpCAM with ab313667 at 1/100 (5.0 ug/ml). Membranous staining on mouse colon. The section was incubated with ab313667 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20532-222] (ab313667)

  Immunohistochemical analysis of paraffin-embedded human colon tissue labeling EpCAM with ab313667 at 1/100 (5.0 ug/ml). Membranous staining on human colon. The section was incubated with ab313667 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20532-222] (ab313667)

  Immunohistochemical analysis of paraffin-embedded human endometrium cancer tissue labeling EpCAM with ab313667 at 1/100 (5.0 ug/ml). Membranous staining on human endometrium cancer. The section was incubated with ab313667 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20532-222] (ab313667)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling EpCAM with ab313667 at 1/100 (5.0 ug/ml). Membranous staining on rat colon. The section was incubated with ab313667 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-EpCAM antibody [EPR20532-222] (ab313667)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling EpCAM with ab313667 at 1/100 (5.0 ug/ml). Negative control: no staining on human skeletal muscle. The section was incubated with ab313667 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins.