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AB196374

Alexa Fluor® 488 Anti-ERG antibody [EPR3864]

4

(4 Reviews)

|

(10 Publications)

Anti-ERG antibody [EPR3864] - Alexa Fluor® 488 conjugated (ab196374) is a rabbit recombinant monoclonal antibody detecting ERG in ICC/IF. Suitable for Human.

- Clone EPR3864 is the most cited clone to ERG
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Transcriptional regulator ERG, Transforming protein ERG, ERG

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (AB196374)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (AB196374)

ab196374 staining ERG in THP-1 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab196374 at 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-ERG antibody [EPR3864]

  • HRP

    HRP Anti-ERG antibody [EPR3864]

  • 578 PE

    PE Anti-ERG antibody [EPR3864]

  • 660 APC

    APC Anti-ERG antibody [EPR3864]

  • Unconjugated

    Anti-ERG antibody [EPR3864]

  • Carrier free

    Anti-ERG antibody [EPR3864] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3864

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

Flow Cyt (Intra), IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in THP-1 cells fixed with 4% formaldehyde (10 min)</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-ERG antibody [EPR3864] (ab196374) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EPR3864 is also available pre-conjugated to a variety of labels for your convenience – Anti-ERG Alexa Fluor® 488 [EPR3864] (ab196374).

Other related products
We have a range of other formats of antibody clone [EPR3864] also available for your convenience: ab92513, HRP - ab195514, Alexa Fluor® 647 - ab196149, Alexa Fluor® 488 - ab196374, ab214341, Carrier free - ab214796, Carrier free - ab275331, PE - ab314261, APC - ab315146

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ERG or ETS-related gene is a transcription factor that belongs to the ETS (E-26 transformation specific) family. The ERG protein plays an important role in gene regulation involved in cell growth and differentiation. ERG has a molecular mass of approximately 54 kDa. It is expressed in a variety of tissues including the prostate endothelial cells and hematopoietic cells. Researchers often study ERG protein localization through techniques such as ERG IHC to better understand its expression across different tissues for example in the ERG color sections that highlight specific cellular contexts.
Biological function summary

ERG regulates processes critical for normal cellular functions such as proliferation apoptosis and differentiation. It functions as part of a larger transcriptional regulatory complex and interacts with other proteins to exert its effects on target genes. The ERG protein can affect angiogenesis and limb development through its regulation of vascular endothelial cells. By controlling such important cellular processes ERG influences both normal physiological functions and abnormal conditions when dysregulated.

Pathways

ERG is highly significant in the MAPK/ERK signaling pathway which is involved in cell division and survival. ERG interacts with other signaling proteins such as FLI1 and RUNX1 affecting cellular responses and contributing to the regulation of gene expression essential for development and homeostasis. Within the hematopoietic context ERG participates in the regulation of pathways that maintain stem cell populations making it important for normal blood cell development.

ERG is prominently implicated in prostate cancer where gene fusions involving ERG lead to its overexpression and contribute to malignancy. The abnormal expression of ERG in endothelial cells can also have a role in vascular disorders. ERG's interplay with proteins like TMPRSS2 often seen in gene fusions in prostate cancer underlines its role in oncogenesis. Understanding how ERG regulates these pathways and its interactions with other proteins helps in developing targeted therapies for disorders involving ERG dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator. May participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
See full target information ERG
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2177-2191 PubMed40858905

2025

ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Erik Ladewig,Matthew Lange,Nazifa Salsabeel,Huiyong Zhao,Young Sun Lee,Anuradha Gopalan,Hanzhi Luo,Wenfei Kang,Ning Fan,Eric Rosiek,Elisa de Stanchina,Yu Chen,Brett S Carver,Christina S Leslie,Charles L Sawyers

Nature communications 16:6788 PubMed40721407

2025

Biomechanical control of vascular morphogenesis by the surrounding stiffness.

Applications

Unspecified application

Species

Unspecified reactive species

Yasuyuki Hanada,Semanti Halder,Yuichiro Arima,Misato Haruta,Honami Ogoh,Shuntaro Ogura,Yukihiko Shiraki,Sota Nakano,Yuka Ozeki,Shigetomo Fukuhara,Akiyoshi Uemura,Toyoaki Murohara,Koichi Nishiyama

Angiogenesis 28:24 PubMed40266369

2025

ApoM-bound S1P acts via endothelial S1PR1 to suppress choroidal neovascularization and vascular leakage.

Applications

Unspecified application

Species

Unspecified reactive species

Bongnam Jung,Hitomi Yagi,Andrew Kuo,Tim F Dorweiler,Masanori Aikawa,Taku Kasai,Sasha A Singh,Andrew J Dannenberg,Zhongjie Fu,Colin Niaudet,Lois E H Smith,Timothy Hla

World journal of surgical oncology 23:131 PubMed40205382

2025

Correlation analysis of tertiary lymphoid structure parameters with the prognosis of patients with locally advanced rectal cancer after neoadjuvant chemotherapy: a retrospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjian Jiang,Chuang Zhang,Yifei Hou,Bin Zhao,Binbin Cui

Nature neuroscience 27:1892-1903 PubMed39210068

2024

Characteristics of blood-brain barrier heterogeneity between brain regions revealed by profiling vascular and perivascular cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah J Pfau,Urs H Langen,Theodore M Fisher,Indumathi Prakash,Faheem Nagpurwala,Ricardo A Lozoya,Wei-Chung Allen Lee,Zhuhao Wu,Chenghua Gu

EMBO molecular medicine 15:e16645 PubMed36912000

2023

Therapeutic activation of endothelial sphingosine-1-phosphate receptor 1 by chaperone-bound S1P suppresses proliferative retinal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Colin Niaudet,Bongnam Jung,Andrew Kuo,Steven Swendeman,Edward Bull,Takahiro Seno,Reed Crocker,Zhongjie Fu,Lois E H Smith,Timothy Hla

eLife 11: PubMed36197001

2022

Murine endothelial serine palmitoyltransferase 1 (SPTLC1) is required for vascular development and systemic sphingolipid homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Kuo,Antonio Checa,Colin Niaudet,Bongnam Jung,Zhongjie Fu,Craig E Wheelock,Sasha A Singh,Masanori Aikawa,Lois E Smith,Richard L Proia,Timothy Hla

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed34353917

2021

Rapid interrogation of cancer cell of origin through CRISPR editing.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Feng,Zhen Cao,Pei Xin Lim,Huiyong Zhao,Hanzhi Luo,Ninghui Mao,Young Sun Lee,Aura Agudelo Rivera,Danielle Choi,Chao Wu,Teng Han,Rodrigo Romero,Elisa de Stanchina,Brett S Carver,Qiao Wang,Maria Jasin,Charles L Sawyers

Nature communications 11:3798 PubMed32732867

2020

A molecular map of murine lymph node blood vascular endothelium at single cell resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Brulois,Anusha Rajaraman,Agata Szade,Sofia Nordling,Ania Bogoslowski,Denis Dermadi,Milladur Rahman,Helena Kiefel,Edward O'Hara,Jasper J Koning,Hiroto Kawashima,Bin Zhou,Dietmar Vestweber,Kristy Red-Horse,Reina E Mebius,Ralf H Adams,Paul Kubes,Junliang Pan,Eugene C Butcher

Developmental cell 52:779-793.e7 PubMed32059774

2020

Sphingosine 1-Phosphate Receptor Signaling Establishes AP-1 Gradients to Allow for Retinal Endothelial Cell Specialization.

Applications

Unspecified application

Species

Unspecified reactive species

Keisuke Yanagida,Eric Engelbrecht,Colin Niaudet,Bongnam Jung,Konstantin Gaengel,Kristina Holton,Steven Swendeman,Catherine H Liu,Michel V Levesque,Andrew Kuo,Zhongjie Fu,Lois E H Smith,Christer Betsholtz,Timothy Hla
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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