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Rat Monoclonal F4/80 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse samples. Cited in 5 publications.

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Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (AB204266), expandable thumbnail

Publications

  • BMC musculoskeletal disorders 22:4942021
    Changes in macrophage and inflammatory cytokine expressions during fracture healing in an ovariectomized mice model.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Lin Chen,Shao Cheng,Kanghui Sun,Jing Wang,Xinhua Liu,Yongjian Zhao,Junjie Yang,Dongfeng Zhao,Chunchun Xue,Yuren Tao,Shitian Zhao,Hao Zhang,Sheng Lu,Qi Shi,Yongjun Wang,Bing Shu
    PubMed 34049522
  • IUBMB life 72:198-2132019
    Downregulation of microRNA-135b promotes atherosclerotic plaque stabilization in atherosclerotic mice by upregulating erythropoietin receptor.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Bo-Wen Wu,Yu Liu,Mi-Shan Wu,Yun-Hui Meng,Meng Lu,Jin-Dong Guo,Yu-Hui Zhou
    PubMed 31444954

Key facts

Isotype

IgG2b

Host species

Rat

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Mouse
Tested

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

This product gave a positive signal in Raw264.7 cells fixed with 100% methanol (5 min).

Associated Products

Select an associated product type

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    2 products for Alternative Version

      Target data

      Function

      Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

      Alternative names

      Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, Adgre1, Emr1, Gpf480

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      Rat Monoclonal F4/80 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse samples. Cited in 5 publications.

      Alternative names

      Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, Adgre1, Emr1, Gpf480

      Key facts

      Isotype

      IgG2b

      Conjugation

      Alexa Fluor® 488

      Excitation/Emission

      Ex: 495nm, Em: 519nm

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      F4/80

      Purity

      IgG fraction

      Light chain type

      kappa

      Concentration
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      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle, Store in the dark

      Notes

      Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

      If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

      Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Supplementary info

      Activity summary

      F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

      Biological function summary

      F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

      Pathways

      F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

      Associated diseases and disorders

      F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

      Product promise

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      1 product image

      • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (ab204266), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (ab204266)

        ab204266 staining F4/80 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204266 at a 1/40 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

        Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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