AB204266

Alexa Fluor® 488 Anti-F4/80 antibody [F4/80]

Name: Anti-F4/80 Alexa Fluor® 488 antibody [F4/80] (ab204266) IgG2b | Abcam
Brand: Abcam Limited
SKU: AB204266

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(8 Publications)

Rat Monoclonal F4/80 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Mouse samples. Cited in 8 publications.

View Alternative Names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

1 Images

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (AB204266)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (AB204266)

ab204266 staining F4/80 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab204266 at a 1/40 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-F4/80 antibody [F4/80]
Unconjugated

Anti-F4/80 antibody [F4/80] - Macrophage Marker

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

F4/80

Isotype

IgG2b

Light chain type

kappa

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p>This product gave a positive signal in Raw264.7 cells fixed with 100% methanol (5 min).</p>" } } }

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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information Adgre1

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 20:4 PubMed39789601

2025

An integrated investigation of mitochondrial genes in COPD reveals the causal effect of NDUFS2 by regulating pulmonary macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Zou,Qiqing Huang,Tutu Kang,Shaoran Shen,Chenxi Cao,Jianqing Wu

Cell reports 43:114925 PubMed39475511

2024

Muscle inflammation is regulated by NF-κB from multiple cells to control distinct states of wasting in cancer cachexia.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin R Pryce,Alexander Oles,Erin E Talbert,Martin J Romeo,Silvia Vaena,Sudarshana Sharma,Victoria Spadafora,Lauren Tolliver,David A Mahvi,Katherine A Morgan,William P Lancaster,Eryn Beal,Natlie Koren,Bailey Watts,Morgan Overstreet,Stefano Berto,Suganya Subramanian,Kubra Calisir,Anna Crawford,Brian Neelon,Michael C Ostrowski,Teresa A Zimmers,James G Tidball,David J Wang,Denis C Guttridge

BMC musculoskeletal disorders 22:494 PubMed34049522

2021

Changes in macrophage and inflammatory cytokine expressions during fracture healing in an ovariectomized mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Chen,Shao Cheng,Kanghui Sun,Jing Wang,Xinhua Liu,Yongjian Zhao,Junjie Yang,Dongfeng Zhao,Chunchun Xue,Yuren Tao,Shitian Zhao,Hao Zhang,Sheng Lu,Qi Shi,Yongjun Wang,Bing Shu

Journal of autoimmunity 119:102612 PubMed33611150

2021

Role of the thymus in spontaneous development of a multi-organ autoimmune disease in human immune system mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mohsen Khosravi-Maharlooei,HaoWei Li,Markus Hoelzl,Guiling Zhao,Amanda Ruiz,Aditya Misra,Yang Li,Nato Teteloshvili,Grace Nauman,Nichole Danzl,Xiaolan Ding,Elisha Y Pinker,Aleksandar Obradovic,Yong-Guang Yang,Alina Iuga,Remi J Creusot,Robert Winchester,Megan Sykes

IUBMB life 72:198-213 PubMed31444954

2019

Downregulation of microRNA-135b promotes atherosclerotic plaque stabilization in atherosclerotic mice by upregulating erythropoietin receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Wen Wu,Yu Liu,Mi-Shan Wu,Yun-Hui Meng,Meng Lu,Jin-Dong Guo,Yu-Hui Zhou

PloS one 14:e0212880 PubMed30811469

2019

Deletion of suppressor of cytokine signaling 3 (SOCS3) in muscle stem cells does not alter muscle regeneration in mice after injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kristy Swiderski,Marissa K Caldow,Timur Naim,Jennifer Trieu,Annabel Chee,René Koopman,Gordon S Lynch

Molecular imaging 16:1536012116689499 PubMed28654380

2017

Targeted Imaging of Tumor-Associated Macrophages by Cyanine 7-Labeled Mannose in Xenograft Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Jiang,Huawei Cai,Xiaodong Peng,Ping Zhang,Xiaoai Wu,Rong Tian

Mediators of inflammation 2017:6541729 PubMed28539706

2017

Multiple Immunosuppressive Effects of CpG-c41 on Intracellular TLR-Mediated Inflammation.

Applications

ICC/IF

Species

Mouse

Wancheng Liu,Xuejiao Yang,Ning Wang,Shijun Fan,Yuanfeng Zhu,Xinchuan Zheng,Yan Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Alexa Fluor® 488 Anti-F4/80 antibody [F4/80]

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] (AB204266)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Conjugation

Excitation/Emission

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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