Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (AB319189)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FLAP with ab319189 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human tonsil.
The section was incubated with ab319189 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (AB319189)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling FLAP with ab319189 at 1/50 (10.0 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in THP-1 cells treated with PMA(80nM 72h) (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (AB319189)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling FLAP with ab319189 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human Hodgkin's lymphoma.
The section was incubated with ab319189 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (AB319189)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling FLAP with ab319189 at 1/50 (10.0 ug/ml) dilution.

Positive staining on immune cells of rat colon.
The section was incubated with ab319189 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (AB319189)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling FLAP with ab319189 at 1/50 (10.0 ug/ml) dilution.

Positive staining on immune cells of mouse colon.
The section was incubated with ab319189 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins