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Rabbit Recombinant Monoclonal FLAP antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (AB319189), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (AB319189), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (AB319189), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (AB319189), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (AB319189), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested
Mouse
Tested
Expected
Rat
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

Required for leukotriene biosynthesis by ALOX5 (5-lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes.

Alternative names

FLAP, ALOX5AP, Arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein

Rabbit Recombinant Monoclonal FLAP antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27505-52
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

FLAP also known as ALOX5AP is an important protein in the leukotriene biosynthetic pathway. It functions mechanically as a membrane-associated protein that facilitates the transfer of arachidonic acid to 5-lipoxygenase (5-LOX) a critical step in the production of leukotrienes. FLAP has a molecular mass of approximately 18 kDa and is primarily expressed in cells of the myeloid lineage such as neutrophils monocytes and macrophages.

Biological function summary

FLAP plays an essential role in the inflammatory response and is a component of the leukotriene synthesis complex. This complex is responsible for the production of leukotrienes which are potent lipid mediators that contribute to inflammation and immune regulation. By regulating leukotriene synthesis FLAP supports processes like chemotaxis cell aggregation and smooth muscle contraction essential for the body's response to inflammation.

Pathways

The FLAP signaling pathway is integral to the leukotriene metabolic pathway. It facilitates the interaction between arachidonic acid and 5-LOX leading to the generation of leukotrienes such as LTA4 and LTB4. These molecules are involved in numerous processes including bronchoconstriction and migration of leukocytes. Proteins related to FLAP within this pathway include 5-LOX and LTC4 synthase which further convert leukotrienes into biologically active compounds.

Associated diseases and disorders

FLAP is associated with asthma and cardiovascular diseases. Its role in producing leukotrienes links it to the pathogenesis of inflammatory conditions. Elevated levels of leukotrienes mediated by FLAP can exacerbate symptoms in asthma by causing bronchial constriction. In cardiovascular diseases FLAP-related pathway dysregulation can contribute to atherosclerosis development. Proteins connected to FLAP through these disorders include leukotriene receptor antagonists which are therapeutic targets for reducing leukotriene-mediated effects.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (ab319189), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (ab319189)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling FLAP with ab319189 at 1/50 (10.0 ug/ml) dilution.

    Positive staining on immune cells of rat colon.

    The section was incubated with ab319189 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (ab319189), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (ab319189)

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling FLAP with ab319189 at 1/50 (10.0 ug/ml) dilution.

    Positive staining on immune cells of mouse colon.

    The section was incubated with ab319189 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (ab319189), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (ab319189)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling FLAP with ab319189 at 1/50 (10.0 ug/ml) dilution.

    Positive staining on human tonsil.

    The section was incubated with ab319189 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (ab319189), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (ab319189)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling FLAP with ab319189 at 1/50 (10.0 ug/ml) dilution (Green).

    Confocal image showing cytoplasmic staining in THP-1 cells treated with PMA(80nM 72h) (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (ab319189), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-FLAP antibody [EPR27505-52] (ab319189)

    Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling FLAP with ab319189 at 1/50 (10.0 ug/ml) dilution.

    Positive staining on human Hodgkin's lymphoma.

    The section was incubated with ab319189 for 60 mins at room temperature (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®.

    The immunostaining was performed on a Leica Biosystems BOND RX instrument.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Counterstained with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 40 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com