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Mouse Monoclonal Frataxin antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

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Images

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (AB156033), expandable thumbnail
  • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (AB156033), expandable thumbnail

Publications

  • Molecular therapy. Methods & clinical development 24:367-3782022
    overexpression of frataxin causes toxicity mediated by iron-sulfur cluster deficiency.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Claudia Huichalaf,Tyler L Perfitt,Anna Kuperman,Renea Gooch,Ramesh C Kovi,Karrie A Brenneman,Xian Chen,Dinesh Hirenallur-Shanthappa,Tiffany Ma,Basel T Assaf,Ingrid Pardo,Tania Franks,Laura Monarski,Ting-Wen Cheng,Kevin Le,Chunyan Su,Suryanarayan Somanathan,Laurence O Whiteley,Christine Bulawa,Marko J Pregel,Alain Martelli
    PubMed 35252470

Key facts

Isotype

IgG1

Host species

Mouse

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 68% PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)
Human
Tested
Mouse
Not recommended
Rat
Not recommended
Cow
Not recommended

Tested
Tested

Species

Human

Dilution info

1/50

Notes

ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Species

Rat

Dilution info

-

Notes

ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Species

Cow

Dilution info

-

Notes

ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

8 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      Promotes the biosynthesis of heme and assembly and repair of iron-sulfur clusters by delivering Fe(2+) to proteins involved in these pathways. May play a role in the protection against iron-catalyzed oxidative stress through its ability to catalyze the oxidation of Fe(2+) to Fe(3+); the oligomeric form but not the monomeric form has in vitro ferroxidase activity. May be able to store large amounts of iron in the form of a ferrihydrite mineral by oligomerization; however, the physiological relevance is unsure as reports are conflicting and the function has only been shown using heterologous overexpression systems. Modulates the RNA-binding activity of ACO1.

      Alternative names

      Friedreich ataxia protein, Fxn, FXN, FRDA, X25

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      Mouse Monoclonal Frataxin antibody - conjugated to Alexa Fluor® 488. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

      Alternative names

      Friedreich ataxia protein, Fxn, FXN, FRDA, X25

      Key facts

      Isotype

      IgG1

      Conjugation

      Alexa Fluor® 488

      Excitation/Emission

      Ex: 495nm, Em: 519nm

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      18A5DB1

      Purity

      IgG fraction

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle, Store in the dark

      Notes

      Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

      If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

      Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

      Product was previously marketed under the MitoSciences sub-brand.

      Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      2 product images

      • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (ab156033), expandable thumbnail

        Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (ab156033)

        Overlay histogram showing HepG2 cells stained with ab156033 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab156033, 1/50 dilution) for 30 min at 22°C.

        Isotype control antibody (black line) was Mouse IgG1 - Alexa Fluor® 488 (ab171463) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

        Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

      • Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (ab156033), expandable thumbnail

        Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Frataxin antibody [18A5DB1] (ab156033)

        Flow cytometric analysis of Human lymphocytes labeling Frataxin with ab156033 at 0.2 µg/ml.

        Lymphocytes were isolated from Control Patients (red), Freidrich Ataxia Carrier Patients (blue), and Friedrich Ataxia Patients (yellow).

        IgG1 Alexa®488 conjugated Isotype control antibody (black at 0.2 µg/ml).

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

      Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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