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AB195188

Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

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(1 Review)

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(11 Publications)

Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - Alexa Fluor® 488 conjugated (ab195188) is a rabbit recombinant monoclonal antibody detecting gamma H2A.X in ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X, H2AS139p, H2AXS139p, H2A.XS139p

1 Images
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195188)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195188)

ab195188 staining Histone H2A.X in Jurkat cells. The cells were incubated with 25uM ETP for 5 hours (Treated) or solvent-only for control purposes (Non-treated). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195188 at 1/50 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • HRP

    HRP Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • Unconjugated

    Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]

  • Carrier free

    Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP854(2)Y

Isotype

IgG

Conjugation

Alexa Fluor® 488

Excitation/Emission

Ex: 495nm, Em: 519nm

Carrier free

No

Reacts with

Human, Human, Mouse, Rat

Applications

ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Sheep": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195188) is a rabbit recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

Related products
Antibody clone EP854(2)Y is also available pre-conjugated to a variety of labels for your convenience – Anti-gamma H2A.X Alexa Fluor® 488 (phospho S139) [EP854(2)Y] (ab195188).

Other related products
We have a range of other formats of antibody clone [EP854(2)Y] also available for your convenience: ab81299, Alexa Fluor® 488 - ab195188, Alexa Fluor® 647 - ab195189, HRP - ab195190, Alexa Fluor® 594 - ab206898, Alexa Fluor® 555 - ab206900, Carrier free - ab215967

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Store in the dark
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.
Biological function summary

Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.

Pathways

Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.

Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
See full target information H2AX phospho S139
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1478-1492 PubMed40490510

2025

Chromothripsis-associated chromosome 21 amplification orchestrates transformation to blast-phase MPN through targetable overexpression of DYRK1A.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte K Brierley,Bon Ham Yip,Giulia Orlando,Jeremy Wen,Sean Wen,Harsh Goyal,Max Levine,G Maria Jakobsdottir,Avraam Tapinos,Alex J Cornish,Antonio Rodriguez-Romera,Alba Rodriguez-Meira,Matthew Bashton,Angela Hamblin,Sally Ann Clark,Joseph C Hamley,Olivia Fox,Madalina Giurgiu,Jennifer O'Sullivan,Lauren Murphy,Assunta Adamo,Aude Anais Olijnik,Anitria Cotton,Emily Hendrix,Shilpa Narina,Shondra M Pruett-Miller,Amir Enshaei,Claire Harrison,Mark Drummond,Steven Knapper,Ayalew Tefferi,Iléana Antony-Debré,James Davies,Anton G Henssen,Supat Thongjuea,David C Wedge,Stefan N Constantinescu,Elli Papaemmanuil,Bethan Psaila,John D Crispino,Adam J Mead

Cancer research 84:3556-3573 PubMed39037997

2024

Targeting YES1 Disrupts Mitotic Fidelity and Potentiates the Response to Taxanes in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina M Piemonte,Natasha N Ingles,Kristen L Weber-Bonk,Mitchell J Valentine,Parth R Majmudar,Salendra Singh,Ruth A Keri

Cancer research communications 3:2233-2243 PubMed37861290

2023

PRMT5 Inhibitors Regulate DNA Damage Repair Pathways in Cancer Cells and Improve Response to PARP Inhibition and Chemotherapies.

Applications

Unspecified application

Species

Unspecified reactive species

Jack Carter,Michael Hulse,Monisha Sivakumar,Jessica Burtell,Venkat Thodima,Min Wang,Anjana Agarwal,Komali Vykuntam,Jacob Spruance,Neha Bhagwat,Joseph Rager,Bruce Ruggeri,Peggy Scherle,Koichi Ito

Journal of ovarian research 16:187 PubMed37684671

2023

The PAPSS1 gene is a modulator of response to cisplatin by regulating estrogen receptor alpha signaling activity in ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Sun,Wei-Xue Ji,Yan Li,Ze-Lian Li,Can-Can Duan,Bai-Rong Xia,Lan Xiao

The Journal of biological chemistry 299:104834 PubMed37201585

2023

Disruption of CDK7 signaling leads to catastrophic chromosomal instability coupled with a loss of condensin-mediated chromatin compaction.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina M Piemonte,Bryan M Webb,Jessica R Bobbitt,Parth R Majmudar,Leslie Cuellar-Vite,Benjamin L Bryson,Nicholas C Latina,Darcie D Seachrist,Ruth A Keri

ACS omega 7:48403-48415 PubMed36591148

2022

In Vitro Toxicological Investigation and Risk Assessment of E-Cigarette Aerosols Based on a Novel Solvent-Free Extraction Method.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjuan Wang,Shulei Han,Huan Chen,Peizhen Li,Shigang Li,Yujuan Wu,Chunxia Zhang,Yaning Fu,Yushan Tian,Tong Liu,Hongwei Hou,Qingyuan Hu

Nature cell biology 24:1099-1113 PubMed35798843

2022

A p53-phosphoinositide signalosome regulates nuclear AKT activation.

Applications

Unspecified application

Species

Unspecified reactive species

Mo Chen,Suyong Choi,Tianmu Wen,Changliang Chen,Narendra Thapa,Jeong Hyo Lee,Vincent L Cryns,Richard A Anderson

Drug design, development and therapy 15:1749-1763 PubMed33953544

2021

MYC/NBS1-Mediated DNA Damage Response is Involved in the Inhibitory Effect of Hydroxysafflor Yellow A on Glioma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dongfang Tang,Tao Huang,Qilong Tian,Julei Wang

Nature communications 12:1536 PubMed33750776

2021

Epigenetic modulation reveals differentiation state specificity of oncogene addiction.

Applications

Unspecified application

Species

Unspecified reactive species

Mehwish Khaliq,Mohan Manikkam,Elisabeth D Martinez,Mohammad Fallahi-Sichani

Cancer immunology research 9:470-485 PubMed33514509

2021

Immunosuppressive Myeloid Cells Induce Nitric Oxide-Dependent DNA Damage and p53 Pathway Activation in CD8 T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Adam N R Cartwright,Shengbao Suo,Soumya Badrinath,Sushil Kumar,Johannes Melms,Adrienne Luoma,Archis Bagati,Assieh Saadatpour,Benjamin Izar,Guo-Cheng Yuan,Kai W Wucherpfennig
View all publications
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Product promise

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