Rabbit Monoclonal H2A.X phospho S139 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 6 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195188), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-gamma-h2ax-phospho-s139-antibody-ep8542y-ab195188--immunocytochemistry-immunofluorescence-img34228.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (AB195188)")
Publications
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- Journal of ovarian research 16:1872023The PAPSS1 gene is a modulator of response to cisplatin by regulating estrogen receptor alpha signaling activity in ovarian cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Sun,Wei-Xue Ji,Yan Li,Ze-Lian Li,Can-Can Duan,Bai-Rong Xia,Lan XiaoPubMed 37684671
- ACS omega 7:48403-484152022In Vitro Toxicological Investigation and Risk Assessment of E-Cigarette Aerosols Based on a Novel Solvent-Free Extraction Method.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongjuan Wang,Shulei Han,Huan Chen,Peizhen Li,Shigang Li,Yujuan Wu,Chunxia Zhang,Yaning Fu,Yushan Tian,Tong Liu,Hongwei Hou,Qingyuan HuPubMed 36591148
- Nature cell biology 24:1099-11132022A p53-phosphoinositide signalosome regulates nuclear AKT activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMo Chen,Suyong Choi,Tianmu Wen,Changliang Chen,Narendra Thapa,Jeong Hyo Lee,Vincent L Cryns,Richard A AndersonPubMed 35798843
- Drug design, development and therapy 15:1749-17632021MYC/NBS1-Mediated DNA Damage Response is Involved in the Inhibitory Effect of Hydroxysafflor Yellow A on Glioma Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongfang Tang,Tao Huang,Qilong Tian,Julei WangPubMed 33953544
- Nature communications 12:15362021Epigenetic modulation reveals differentiation state specificity of oncogene addiction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMehwish Khaliq,Mohan Manikkam,Elisabeth D Martinez,Mohammad Fallahi-SichaniPubMed 33750776
- Cancer immunology research 9:470-4852021Immunosuppressive Myeloid Cells Induce Nitric Oxide-Dependent DNA Damage and p53 Pathway Activation in CD8 T Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdam N R Cartwright,Shengbao Suo,Soumya Badrinath,Sushil Kumar,Johannes Melms,Adrienne Luoma,Archis Bagati,Assieh Saadatpour,Benjamin Izar,Guo-Cheng Yuan,Kai W WucherpfennigPubMed 33514509
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Sheep | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
5 products for Alternative Version
Target data
Function
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Alternative names
H2AFX, H2AX, Histone H2AX, H2a/x, Histone H2A.X, H2AS139p, H2AXS139p, H2A.XS139p
Recommended products
Rabbit Monoclonal H2A.X phospho S139 antibody - conjugated to Alexa Fluor® 488. Suitable for ICC/IF and reacts with Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP854(2)Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Gamma H2A.X also known as phospho H2A.X or γH2A.X is a phosphorylated form of the histone variant H2A.X. It has a molecular weight of about 14 kilodaltons and occurs primarily in they nucleus. When DNA double-strand breaks (DSBs) occur serine 139 in H2A.X undergoes rapid phosphorylation resulting in gamma H2A.X. This modification happens swiftly at the site of damage and gamma H2A.X spreads over a large chromatin area facilitating the recruitment of DNA repair proteins. Gamma H2A.X staining typically evaluated using gamma H2A.X immunofluorescence techniques aids in identifying the presence and extent of DNA damage.
- Biological function summary
Gamma H2A.X plays a role in DNA damage response and repair. It does not operate alone; it acts as part of a complex with other repair proteins. The formation of gamma H2A.X foci at DNA damage sites creates a signal attracting repair factors that help maintain genome stability. Its interaction with MDC1 and ATM proteins exemplifies its significant role in orchestrating an effective response to DNA damage. Beyond DNA repair gamma H2A.X influences cell cycle checkpoints permitting cells to pause and repair before proceeding with division.
- Pathways
Gamma H2A.X plays a pivotal role in the DNA damage response (DDR) pathway. This pathway is essential for detecting and repairing DNA lesions to uphold genomic integrity. Within the DDR pathway gamma H2A.X is closely associated with proteins such as NBS1 and BRCA1 which assist in repairing double-strand breaks. In addition gamma H2A.X is integral to the ATM-ATR signaling pathway where its activation promotes cell survival following genotoxic stress by signaling for damage repair or triggering apoptosis.
- Associated diseases and disorders
Gamma H2A.X has connections to cancer and neurodegeneration. Aberrant DNA repair pathways often indicated by persistent gamma H2A.X signals correlate with tumor formation and progression. For instance a failure to repair DNA damage effectively can lead to mutations that drive cancer development. Gamma H2A.X also links to neurodegenerative diseases where dysregulated DNA repair contributes to neuronal cell death. Proteins like p53 which regulate cell cycle and apoptosis further connect to gamma H2A.X bridging its role in disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195188), expandable thumbnail](https://content.abcam.com/products/images/alexa-fluor-488-gamma-h2ax-phospho-s139-antibody-ep8542y-ab195188--immunocytochemistry-immunofluorescence-img34228.jpg "Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195188)")
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y] (ab195188)
ab195188 staining Histone H2A.X in Jurkat cells. The cells were incubated with 25uM ETP for 5 hours (Treated) or solvent-only for control purposes (Non-treated). The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 10% normal goat serum in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab195188 at 1/50 dilution (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/200 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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