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Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-Fr, IHC-P and reacts with Rat samples. Cited in 4 publications.


Images

Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (AB194324), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (AB194324), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (AB194324), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (AB194324), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrIHC-P
Human
Predicted
Predicted
Mouse
Predicted
Predicted
Rat
Tested
Tested

Tested
Tested

Species
Rat
Dilution info
1/50
Notes

-

Predicted
Predicted

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Mouse, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

8 products for Alternative Version

Target data

Function

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Alternative names

Glial fibrillary acidic protein, GFAP

Recommended products

Rabbit Recombinant Monoclonal GFAP antibody - conjugated to Alexa Fluor® 488. Suitable for IHC-Fr, IHC-P and reacts with Rat samples. Cited in 4 publications.

Key facts

Isotype
IgG
Conjugation
Alexa Fluor® 488
Excitation/Emission
Ex: 495nm, Em: 519nm
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR1034Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle, Store in the dark

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (ab194324), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (ab194324)

    IHC-Fr image of GFAP staining in a section of frozen rat brain (hypothalamus). The section was air dried on the bench for 30 minutes and blocked for 2 hours in 1XPBS/0.1% Tween 20/1% BSA/0.3M Glycine buffer. The sample was then incubated overnight at +4°C with ab194324 at 1/50 dilution (shown in green). DAPI-containing mounting media was added to the sample (shown in blue). The inset negative control image is taken from an identical assay without primary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (ab194324), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (ab194324)

    Image A: composite multiplex immunohistochemistry staining using conjugates panel composed of anti- GFAP (Alexa Fluor® 488) (ab194324, 1/100 dilution, 5 µg/mL) (green), anti-MOG (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] ab305372, 1/100 dilution, 5 µg/mL) (magenta) and anti-Iba1 (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588] ab225261, 1/100 dilution, 5 µg/mL) (white) on Formalin/PFA-fixed paraffin-embedded sections of rat cerebellum. Nuclear DNA was labelled with DAPI (blue).

    Image B: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti- GFAP (Alexa Fluor® 488) (ab194324, 1/100 dilution, 5 µg/mL).

    Image C: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti- MOG (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-Myelin oligodendrocyte glycoprotein antibody [EPR22629-310] ab305372, 1/100 dilution, 5 µg/mL).

    Image D: Formalin/PFA-fixed paraffin-embedded section of rat cerebellum stained with anti- Iba1 (Alexa Fluor® 647) (Alexa Fluor® 647 Anti-Iba1 antibody [EPR16588] ab225261, 1/100 dilution, 5 µg/mL).

    Immunohistochemistry staining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with EDTA (pH 9.0, 110°C) for 40 mins. The section was then incubated at room temperature for 1 hour and mounted using Dako Fluorescence Mounting Medium®.

    Image acquisition was performed with Leica SP8 confocal microscope. For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (ab194324), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (ab194324)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling GFAP with Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining in mouse primary glia cells.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab194324 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (ab194324), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-GFAP antibody [EPR1034Y] (ab194324)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling GFAP with Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596 at 1/50 (10.0 ug/ml) dilution (Green). Confocal image showing positive staining in rat primary glia cells.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. ab194324 Anti-GFAP rabbit monoclonal antibody (Alexa Fluor® 488) was used to counterstain tubulin at 1/100 5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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